Validated Spectrofluorometric Method for Determination of Atenolol in Pharmaceutical Preparations


  • Ataturk University, Department of Analytical Chemistry, Faculty of Pharmacy, Erzurum, 25240, Turkey


In this study, a new and rapid spectrofluorometry method was developed for determination of atenolol in pure and pharmaceutical preparation. The solvent system and wavelength of detection were optimized in order to maximize the sensitivity of the proposed method. Parameters such as linearity, precision, accuracy, specificity, stability, limit of detection and limit of quantification were studied according to the International Conference on Harmonization (ICH) Guidelines. The linearity was established between the concentration range of 50-4000 ng/mL. The intra- and inter-day relative standard deviation (RSD) was less than 2.97%. Limits of detection and quantification were determined as 15.2 and 46.1 ng/mL, respectively. The mean recovery value of atenolol was 99.3% for pharmaceutical preparation. The method was applied for the quality control of commercial atenolol dosage form to quantify the drug and to check the formulation content uniformity.


Atenolol, Spectrofluorometry, Validation, Tablet.

Subject Discipline

Pharmacy and Pharmacology

Full Text:


Al-Ghannam SM, A simple spectrophotometric method for the determination of β-blockers in dosage forms, J. Pharm. Biomed. Anal. 40, 2006, 151-156.

Reynolds JEF, In: Martindale, The Extra Pharmacopeia (31st ed.), Royal Pharmaceutical Society, London, 1996, 827.

Singh S, Jain R, Simultaneous spectrophotometric determination of atenolol and amlodipine besylate from dosage forms, Indian Drugs, 34, 1997, 678679.

Bonazzi D, Gotti R, Andrisano V, Cavrini V, Derivative UV spectrophotometric determination of atenolol and metoprolol in singleand multicomponent pharmaceutical dosage forms, Farmaco, 51, 1996, 733-738.

Wehner W, Determination of atenolol and chlorthalidone during dissolution of tablets with UV multicomponent analysis, Pharmazie, 55, 2000, 543-544.

Ceresole R, Moyano MA, Pizzorno MT, Segall AI, Validated reversed-phase HPLC method for the determination of atenolol in the presence of its major degradation product. J. Liq. Chrom. Relat. Tech. 2006, 3009-3019.

Argekar AP, Powar SG, Simultaneous determination of atenolol and amlodipine in tablets by highperformance thin-layer chromatography, J. Pharm. Biomed. Anal. 21, 2000, 1137-1142.

Keech AC, Harrison PM, Mclean AJ, Simple extraction of atenolol from urine and its determination by high-performance liquid chromatography, J. Chromatogr. 426, 1988, 234236.

Leloux MS, Dost F, Doping analysis of beta-blocking drugs using high-performance liquid chromatography, Chromatographia, 32, 1991, 429- 435.

Goyal RN, Gupta VK, Oyama M, Bachheti N, Diferential pulse voltammetric determination of atenolol in pharmaceutical formulations and urine using nanogold modified indium tin oxide electrode, Electrochem. Commun. 8, 2006, 6570.

Arvand M, Vejdani M, Moghimi M, Construction and performance characterization of an ion selective electrode for potentiometric determination of atenolol in pharmaceutical preparations, Desalination, 225, 2008, 176-184.

The European Agency for the Evaluation of Medicinal Products. ICH Topic Q2B Note for Guideline on Validation of Analytical Procedures: Methodology GPMP/ICH/281/95, 1996.

The British Pharmacopoeia, Her Majesty Stationary Office, London, 1993.


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