Cardioprotective Activity of Methanol Extract of Fruit of Trichosanthes cucumerina on Doxorubicin-induced Cardiotoxicity in Wistar Rats


Affiliations

  • Bharati Vidyapeeth Deemed University Medical College & Hospital, Department of Pharmacology, Poona College of Pharmacy, Pune, Maharashtra, India
  • Bharati Vidyapeeth Deemed University, Department of Pharmacology, Poona College of Pharmacy, Pune, Maharashtra, India

Abstract

Aim and Objective: The objective was to determine the activity of methanol extract of fruit of Trichosanthes cucumerina in doxorubicin-induced cardiotoxicity in rats. Materials and Methods: The methanol extract of fruit of T. cucumerina was prepared. Male Wistar rats were divided in four groups. Group Ι was vehicle control. Group ΙΙ animals received doxorubicin 4 mg/kg i.p. on days 21, 28, 35, and 42. Group ΙΙΙ and ΙV animals were treated with methanol extract of T. cucumerina (500 and 1000 mg/kg, respectively) for 49 days. Doxorubicin was administered on days 21, 28, 35, and 42 days. The parameters of study were body weight, serum biomarkers, ECG, blood pressure, and left ventricular function. At the end of the study, the histology of heart, liver, and kidney was carried out. Results: Cardiac toxicity by doxorubicin was manifested as body weight loss, elevated serum LDH and CK-MB, increased ST, QT and QRS complex, reduced blood pressure, and left ventricular function. The methanol extract of T. cucumerina significantly decreased LDH and CK-MB, reduced ST, QT interval and QRS complex, increased heart rate, restored blood pressure, and left ventricular function. Doxorubicin caused liver and kidney necrosis, cellular infiltration, and vascular changes that indicated injury. Conclusion: T. cucumerina (1000 mg/kg) reduced the severity of doxorubicin-induced cardiac damage especially in heart. It is concluded that doxorubicin-induced cardiotoxicity is reduced by pretreatment with methanol extract of fruit of T. cucumerina.

Keywords

Cardiotoxicity, doxorubicin, Trichosanthes cucumerina

Subject Discipline

Zoology

Full Text:

References

Adebooye OC. Phyto-constituents and anti-oxidant activity of the pulp of snake tomato (Trichosanthes cucumerina L.). Afr J Tradit Complement Altern Med 2008;5:173-9.

Al-Majed AA, Gdo AM, Al-Shabanah OA, Mansour MA. Alphalipolic acid ameliorate myocardial toxicity induced by doxorubicin. Pharmacol Res 2002;46:499-503.

Arawwawala M, Thabrew I, Arambewela L, Handunnetti S. Antiinflammatory activity of Trichosanthese cucumerina Linn. in rats. J Ethanopharmacol 2010;131:538-43.

Arawwala M, Thabrew I, Arambewela L. Gastroprotective activity of Trichosanthese cucumerina in rats. J Ethanopharmacol 2010;127:750-54.

Ateşşahin A, Karahan I, Türk G, Gür S, Yilmaz S, Ceribaşi AO. Protective role of lycopene on cisplatin-induced changes in spermcharacteristics, testicular damage and oxidative stress in rats. Reprod Toxicol 2006;21:42-7.

Atessahin A, Yilmaz S, Karahan I, Ceribasi AO, Karaoglu A. Effects of lycopene against cisplatin-induced nephrotoxicity and oxidative stress in rats. Toxicology 2005;212:116-23.

De Beer EL, Bottone AE, Voest EE. Doxorubicin and mechanical performance of cardiac trabeculae after acute and chronic treatment: A review. Eur J Pharmacol 2001;415:1-11.

Elberry AA, Abdel-Naim AB, Abdel-Sattar EA, Nagy AA, Mosli HA, Mohamadin AM, et al. Cranberry (Vaccinium macrocarpon) protects against doxorubicin-induced cardiotoxicity in rats. Food Chem Toxicol 2010;48:1178-84.

Hassanpour F, Bodhankar SL, Dikshit M. (2008). Cardioprotective activity of fruit of Lagenaria siceraria (Molina) Standley on doxorubicine induced cardiotoxicity in rats. Int J Pharmacol, 4, 466-471.

Alkreathy H, Damanhouri ZA, Ahmed N, Slevin M, Ali SS, Osman AM. Aged garlic extract protects against doxorubicin-induced cardiotoxicity in rats. Food Chem Toxicol 2010;48:951-6.

James YK. Molecular and cellular mechanism of carditoxicity. Environ Health Perspect 2001;109:27-33.

Jiang B, Zhang L, Li M, Wu W, Yang M, Wang J, et al. Salvinolic acids prevent acute doxorubicin cardiotoxicity in mice through suppression of oxidative stress. Food Chem Toxicol 2008;46:1510-5.

Kirana H, Srinivasan BP. Trichosanthese cucumerina Linn. improves glucose tolerance and tissue glucose in noninsulin dependent diabetes mellitus induced rats. Indian J Pharmacol 2008;40;103-6.

Sathesh Kumar S, Ravi Kumar B, Krishna Mohan G. Hepatoprotective effect of Trichosanthes cucumerina Var cucumerina L. on carbon tetrachloride induced liver damage in rats. J Ethanopharmacol 2009;123:347-50.

Lindley MG. The impact of food processing on antioxidants in vegetable oils, fruits and vegetables. Trend Food Sci Technol 1998;9:336-40.

Antunes LM, Takahashi CS. Effects of high doses of vitamins C and E against doxorubicin-induced chromosomal damage in wistar rat bone marrow cells. Mutat Res 1998;419:317-43.

Yagmurca M, Fadillioglu E, Erdogan H, Ucar M, Sogut S, Irmak MK. Erdosteine prevents doxorubicin-induced cardiotoxicity in rats. Pharmacol Res 2003;48:377-82.

Namiki M. Antioxidant/antimutagens in food. Crit Rev Food Sci Nutr 2009;29:273-300.

Olson RD, Mushlin PS, Brenner DE, Fleischer S, Cusack BJ, Chang BK, et al. Doxorubicin cardiotoxicity may be caused by its metabolite, doxorubicinol. Proc Natl Acad Sci U S A 1998;85:3585-9.

Osman AM, Nemnem MM, Abou-Bakr AA, Nassier OA, Khayyal MT. Effect of methimazole treatment on doxorubicin-induced cardiotoxicity in mice. Food Chem Toxicol 2009;47:2425-30.

Reddy LJ, Jose B, Anjana JC, Ruveena TN. Evaluation of antibacterial activity of Trichosanthes cucumerina l. and Cassia didymobotrya fres. Leaves. Int J Pharm Pharma Sci 2010;2:153-55.

Kilickap S, Akgul E, Aksoy S, Aytemir K, Barista I. Doxorubicin induced second degree and complete atrioventricular block. Europace 2005;7:227-30.

Sandhya S, Vinod KR, Chandra Sekhar J, Aradhana R, VamshiSarathNath. An updated review on Tricosanthes cucumerina. Int J of Pharma Sci. Rev. Res, 2010;1:56-60.

Sarada S, Dipti P, Anuj B, Pauline T, Kain AK, Sairam M, et al. Antioxidant effect of beta- carotene on hypoxia induced oxidative stress in male albino rats. J Ethnopharmacol 2002;79:149-53.

Shuai Y, Guo JB, Peng SQ, Zhang LS, Guo J, Han G, et al. Metallothionein protects against doxorubicin-induced cardiomyopathy through inhibition of superoxide generation and related nitrosative impairment. Toxicol Lett 2007;170:66-74.

Tehila T, Margalit B, Shlomo G. Cucurbitacin glycoside: Antioxidant and free-radical scavenging activities. Biochem Biophys Res Commun 2007;364:181-6.

Teow CC, Truong V, Mcfeeters RF, Thompson RL, Pecota KV, Yencho GC. Antioxidant activities, phenolic and β-carotene contents of sweet potato genotypes with varying flesh colours. Food Chem 2007;103:829-38.

Hou XW, Jiang Y, Wang LF, Xu HY, Lin HM, He XY, et al. Protective role of granulocyte colony-stimulating factor against adriamycin induced cardiac, renal and hepatic toxicities. Toxicol Lett 2009;187:40-4.

Zhang D, Hamauzu Y. Phenolics, ascorbic acid, carotenoids and antioxidant activity of broccoli and their changes during conventional andmicrowave cooking. Food Chem 2004;88:503-9.


Refbacks

  • There are currently no refbacks.