Effect of Trans-resveratrol on Rotenone-induced Cytotoxicity in Human Breast Adenocarcinoma Cells

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Authors

  • M. A. Siddiqui
     A.R. Al-Jeraisy Chair for DNA Research, College of Science, King Saud University, Riyadh ,SA
  • Q. Saquib
     A.R. Al-Jeraisy Chair for DNA Research, College of Science, King Saud University, Riyadh ,SA
  • M. Ahamed
     King Abdullah Institute for Nanotechnology, King Saud University, Riyadh ,SA
  • J. Ahmad
     A.R. Al-Jeraisy Chair for DNA Research, College of Science, King Saud University, Riyadh ,SA
  • A. A. Al-Khedhairy
     Department of Zoology, College of Science, King Saud University, Riyadh ,SA
  • F. M. Abou-Tarboush
     Department of Zoology, College of Science, King Saud University, Riyadh ,SA
  • J. Musarrat
     Department of Microbiology, Faculty of Agricultural Sciences, AMU, Aligarh ,IN

Keywords:

Cytotoxicity, MCF--7 cells, rotenone, trans-resveratrol
Behavioral ecology

Abstract

Rotenone, a botanical insecticide is known to cause apoptosis in various cell types. Trans-resveratrol, a natural phytophenol present in red grapes and wine, is also well documented for its antioxidant, anti-inflammatory, anti-mutagenic, and anticarcinogenic activities. Therefore, the present investigations were carried out to assess the protective effect of trans-resveratrol against rotenone-induced cell death in human breast adenocarcinoma (MCF-7) cells. MCF-7 cells were exposed with various concentrations of rotenone for 24 h, and the loss in percent cell viability was evaluated by MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] and neutral red uptake (NRU) assays. A significant decrease in percent cell viability in MCF-7 cells was observed at 50 µM and above concentrations of rotenone, as compared to untreated control. Furthermore, various concentrations (5, 10, and 25 µÎœ) of trans-resveratrol were used to see its protective role on cell viability in rotenone-induced cell death in MCF-7 cells. Pre- or post- treatment of trans-resveratrol for 24 h was given to the cells. The data exhibited a significant dose dependent increase in the percent cell viability under pre- and post-treatment conditions. However, post-treatment of trans-resveratrol for 24 h after rotenone exposure to the cells was relatively less effective. Overall, the results suggest that trans-resveratrol significantly protects MCF-7 cells from rotenone-induced cell death. This model can be used as an effective and economical alternative to animal models for screening the antioxidant activity of a variety of natural compounds/drugs.

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Published

2018-05-18

How to Cite

Siddiqui, M. A., Saquib, Q., Ahamed, M., Ahmad, J., Al-Khedhairy, A. A., Abou-Tarboush, F. M., & Musarrat, J. (2018). Effect of <i>Trans</i>-resveratrol on Rotenone-induced Cytotoxicity in Human Breast Adenocarcinoma Cells. Toxicology International, 18(2), 105–110. Retrieved from http://www.informaticsjournals.com/index.php/toxi/article/view/21253

Issue

Volume 18, Issue 2, July-December 2011

Section

Articles
Received 2018-05-17
Accepted 2018-05-17
Published 2018-05-18

 

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