Pharmacodynamic Interaction of Green Tea Extract with Hydrochlorothiazide against Cyclophosphamide‑Induced Myocardial Damage


Affiliations

  • Bhagwant University, Research Scholar, Department of Pharmacology, Ajmer, India
  • Shree Devi College of Pharmacy, Department of Pharmacology, Mangalore, Karnataka, India

Abstract

Objective: Treatment of ischemic hypertensive patients with hydrochlorothiazide can precipitate cardiac arrhythmias. Green tea by virtue of its antioxidant potential is responsible for cardio‑protective activity. The present study was undertaken to evaluate the pharmacodynamic interaction of green tea extract with hydrochlorothiazide against cyclophosphamide‑induced myocardial toxicity. Materials and Methods: Rats were treated with high (500 mg/kg, p.o.) and low (100 mg/kg, p.o.) dose of green tea extract in alone and interactive groups for 10 days. Standard, high, and low dose of interactive groups received hydrochlorothiazide (10 mg/kg, p.o.) for last 7 days. Apart from normal control, all other groups were subjected to cyclophosphamide (200 mg/kg, i.p.) toxicity on day first and the effects of different treatments were evaluated by changes in electrocardiographic parameters, serum biomarkers, and tissue antioxidant levels. Apart from that, lipid profile and histological studies were also carried out. Results: Compared to cyclophosphamide control group, both high and low dose of green tea exhibited significant decrease in serum biomarkers and increase in tissue antioxidant levels. Green tea treatment was also responsible for significant improvement in echocardiography (ECG) parameter, lipid profile, and histological score. Incorporation of high and low dose of green tea with hydrochlorothiazide‑exhibited significant protection compared to hydrochlorothiazide‑alone‑treated group. Conclusion: The present findings clearly suggested that green tea extract dose dependently reduces cyclophosphamide‑induced myocardial toxicity. Green tea when combined with hydrochlorothiazide can reduce the associated side effects and exhibits myocardial protection.

Keywords

Cyclophosphamide, green tea, hydrochlorothiazide

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References

Todorova V, Vanderpool D, Blossom S, Nwokedi E, Hennings L, Mrak R, et al. Oral glutamine protects against cyclophosphamide‑induced cardiotoxicity in experimental rats through increase of cardiac glutathione. Nutrition 2009;25:812‑7.

Shanholtz C. Acute life‑threatening toxicity of cancer treatment. Crit Care Clin 2001;17:483‑502.

Schimmel KJ, Richel DJ, van den Brink RB, Guchelaar HJ. Cardiotoxicity of cytotoxic drugs. Cancer Treat Rev 2004;30:181‑91.

Loudet AM, Dousset N, Carton M, Douste‑Blazy L. Effects of an antimitotic agent (cyclophosphamide) on plasma lipoproteins.Biochem Pharmacol 1984;33:2961‑5.

Cheng L, Ding G, Qin Q, Huang Y, Lewis W, He N, et al.Cardiomyocyte‑restricted peroxisome proliferator activated receptor‑delta deletion perturbs myocardial fatty acid oxidation and leads to cardiomyopathy. Nat Med 2004;10:1245‑50.

Asdaq SM, Inamdar MN. The potential for interaction of hydrochlorothiazide with garlic in rats. Chem Biol Interact 2009;181:472‑9.

Berman AF, Ernst E. Herb–drug interactions: Review and assessment of report reliability. Br J Clin Pharmacol 2001;52:587‑95.

Cabrera C, Artacho R, Giménez R. Beneficial effects of green tea‑‑a review. J Am Coll Nutr 2006;25:79‑99.

Brown MD. Green tea (Camellia sinensis) extract and its possible role in the prevention of cancer. Altern Med Rev 1999;4:360‑70.

Papparella I, Ceolotto G, Montemurro D, Antonello M, Garbisa S, Rossi G, et al. Green tea attenuates angiotensin II‑induced cardiac hypertrophy in rats by modulating reactive oxygen species production and the Src/epidermal growth factor receptor/Akt signaling pathway. J Nutr 2008;138:1596‑601.

Antonello M, Montemurro D, Bolognesi M, Di Pascoli M, Piva A, Grego F, et al. Prevention of hypertension, cardiovascular damage and endothelial dysfunction with green tea extracts. Am J Hypertens 2007;20:1321‑8.

Nakagawa K, Ninomiya M, Okubo T, Aoi N, Juneja LR, Kim M, et al. Tea catechin supplementation increases antioxidant capacity and prevents phospholipid hydroperoxidation in plasma of humans. J Agric Food Chem 1999;47:3967‑73.

Patil L, Balaraman R. Effect of green tea extract on Doxorubicin induced cardiovascular abnormalities: Antioxidant action. Iran J Pharm Res 2011;10:89‑96.

Finar IL. Organic Chemistry; Vol 1: 4th ed. ELBS; 1993. p. 518.

Mukherjee PK. Quality Control of Herbal Drugs – An Approach to Evaluation of Botanicals. 1st ed, New Delhi: Business Horizons; 2002. p. 246.

Chakraborty M, Asdaq SM. Interaction of Semecarpus anacardium L. with propranolol against isoproterenol induced myocardial damage in rats. Indian J Exp Biol 2011;49:200‑6.

Viswanatha Swamy AH, Patel UM, Koti BC, Gadad PC, Patel NL, Thippeswamy AH. Cardioprotective effect of Saraca indica against cyclophosphamide induced cardiotoxicity in rats: A biochemical, electrocardiographic and histopathological study. Indian J Pharmacol 2013;45:44‑8.

Field MJ, Stanton BA, Giebisch GH. Differential acute effects of aldosterone, dexamethasone, and hyperkalemia on distal tubular potassium secretion in the rat kidney. J Clin Invest 1984;74:1792‑802.

Hoes AW, Grobbee DE, Peet TM, Lubsen J. Do non‑potassium‑sparing diuretics increase the risk of sudden cardiac death in hypertensive patients? Recent evidence. Drugs 1994;47:711‑33.

Hernández Figueroa TT, Rodríguez‑Rodríguez E, Sánchez‑Muniz FJ. The green tea, a good choice for cardiovascular disease prevention? Arch Latinoam Nutr 2004;54:380‑94.

Shanmugarajan TS, Arunsunder M, Somasundaram I, Krishnakumar E, Sivaraman D, Ravichandiran V. Protective effect of Ficus hispida Linn. on cyclophosphamide provoked oxidative myocardial injury in rat model. Int J Pharmacol 2008;1:1‑10.

Sekeroğlu V, Aydin B, Sekeroğlu ZA. Viscum album L. extract and quercetin reduce cyclophosphamide‑induced cardiotoxicity, urotoxicity and genotoxicity in mice. Asian Pac J Cancer Prev 2011;12:2925‑31.

Atlee JL. Protective cardiac dysrhythmias: Diagnosis and management. Anesthesiology 1997;86:1397‑424.

Levine ES, Friedman HS, Griffith OW, Colvin OM, Raynor JH, Lieberman M. Cardiac cell toxicity induced by 4‑hydroperoxycyclophosphamide is modulated by glutathione.Cardiovasc Res 1993;27:1248‑53.


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