Protective Effect of Caffeine on Ethyl Methanesulfonate"‘Induced Wing Primordial Cells of Drosophila Melanogaster

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  • ,IN

Keywords:

Antimutagenicity, caffeine, drosophila melanogaster, ethyl methanesulfonate

Abstract

Objectives: The antimutagenic effect of caffeine is evaluated against ethyl methanesulfonate (EMS)-induced mutation rate in Drosophila. Materials and Methods: The mutation rate is evaluated using wing mosaic assay. In transheterozygous larvae, multiple wing hair (mwh 0.3-3) and flare (flr 3-38.8) genes were used as markers of the extent of mutagenicity. Results: The results at 0.5 and 1.0 mM EMS concentration at both 48 ± 4 and 72 ± 4 h have shown consistent increase in mutation rate, which was being measured as frequency of clone formation per 105 cells. Toxicity of caffeine at 5 mM concentration was parallel to that of distilled water alone. At 0.5 mM EMS concentration at 42 ± 4 and 72 ± 4 h, Drosophila larvae mutation rate was significantly increased. Although caffeine prevented mutation rate in all pre, post, and combined treatment, it was more significant in pretreatment experiments where it was found to be effective in reducing the genotoxicity of EMS. However, the concentration of caffeine as recommended in dietary allowance did not induce the frequency of mutant clones in somatic mutation and recombination test (SMART) recorded. Conclusion: This study shows that caffeine significantly reduced the genotoxicity induced by EMS. However, the limitation in completely abolishing genotoxicity induced by EMS as observed at the dietary allowance of caffeine makes it interesting for further in-depth study. Further studies on the molecular mechanism of antigenotoxic effect of caffeine will also be interesting.

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Published

2018-04-25

How to Cite

Prakash, G., Hosetti, B. B., & Dhananjaya, B. L. (2018). Protective Effect of Caffeine on Ethyl Methanesulfonate"‘Induced Wing Primordial Cells of Drosophila Melanogaster. Toxicology International, 21(1), 96–100. Retrieved from http://www.informaticsjournals.com/index.php/toxi/article/view/20982

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Section

Original Research
Received 2018-04-24
Accepted 2018-04-24
Published 2018-04-25

 

References

Ferguson LR. Antimutagens as cancer chemoprevntive agent in the diet. Mutat Res 1994;307:395-410.

Mitscher LA, Telikepalli H, McGhee E, Shankel DM. Natural antimutagenic agents. Mutat Res 1996;350:143-52.

Ikken Y, Morales P, Martí­nez A, Marí­n ML, Haza AI, Cambero MI. Antimutagenic effect of fruit and vegetable ethanolic extracts against n-nitrosamines evaluated by the ames test. J Agric Food Chem 1999;47:3257-64.

Abrahm SK. Inhibitory effects of coffee in the genotoxcity of carcinogens in mice. Mutat Res 1991;262:109-14.

Abrahm SK. Inhibition of in vivo genotoxcity by coffee. Food Chem Toxicol 1989;27:787-92.

Aeschbacher HU, Jaccaud E. Inhibition by coffee of nitrosoureamediated DNA damage in mice. Food Chem Toxicol 1990;28:633-7.

Coffee, tea, mate, methylxanthines and methylglyoxal. IARC Working Group on the Evaluation of Carcinogenic Risks to Humans. Lyon, 27 February to 6 March 1990. IARC Monogr Eval Carcinog Risks Hum; 1991. p. 513.

Stavric B. An update on research with coffee/caffeine (1989-1990). Food Chem Toxicol 1992;30:533-55.

Devasayagam TP, Kesavan PC. Radioprotective and antioxidant action of caffeine: Mechanistic considerations. Indian J Exp Biol 1996;34:291-7.

Wattenberg LW. Chemoprevention of carcinogenesis by minor dietary constituents: Symposium introduction. Pharm Bull 1998;36:S6-7.

Shi X, Dalal NS, Jain AC. Antioxidant behavior of caffeine: Efficient scavenging of hydroxyl radicals. Food Chem Toxicol 1991;29:1-6.

Devasayagam TP, Kamath JP, Mohan H, Kesavan PC. Caffeine as an antioxidant: Inhibition of lipid peroxidation induced by reactive oxygen species. Biochem Biophys Acta 1996;1282:63-70.

Mohr U, Emura M, Riebe-Imre M. Experimental studies on carcinogenicty and mutagenicity of caffeine. In: Garattini S, editor. New York: Caffeine, Coffee and Health, Revan; 1993. p. 359-78.

Abrahm SK. Antigenotoxcity of coffee in the Drosophila assay for somatic mutation and recombination. Mutagenesis 1994;9:383-6.

In: Lindsley DL, Zimm GG, editors. The Genome of Drosophila melanogaster. San Diego: Academic Press; 1992. p. 1133.

Graf U, Würgler FE, Katz AJ, Frei H, Juon H, Hall CB, Kale PG. Somatic mutation and recombination test in Drosophila melanogaster. Mutat Res 1984;271:59-67.

Frei H, Clements J, Howe D, Würgler FE. The genotoxicity of anti-cancer drug mitoxantrone in somatic and germ cells of Drosophila melanogaster. Mutat Res 1992;279:21-33.

Frei H, Würgler FE. Statistical methods to decide whether mutagenicity test data from Drosophila assays indicate a positive, negative, or inconclusive result. Mutat Res 1988;203:297-308.

Frei H, Würgler FE. Optimal experimental design and sample size for the statistical evaluation of data from somatic mutation and recombination test (SMART) in Drosophila. Mutat Res 1995;334:247-58.

Kastenbaum MA, Bowman KO. Tables for determining thestatistical significance of mutation frequencies. Mutat Res 1970;9:527-49.

Moraga AA, Graf U. Genotoxicity testing of antiparasitic nitrofurans in the Drosophila wing somatic mutation and recombination test. Mutagenesis 1989;4:105-10.

Santos JH, Graf U, Reguly ML, Rodrigues de Andrade HH. The synergistic effects of vanillin on recombination predominate over its antimutagenic action in relation to MMC-induced lesions in somatic cells of Drosophila melanogaster. Mutat Res 1999;444:355-65.

Sinigaglia M, Lehmann M, Baumgardt P, do Amaral VS, Dihl RR, Reguly ML, et al. Vanillin as a modulator agent in SMART test: Inhibition in the steps that precede N-methyl-Nnitrosourea-, N-ethyl-N-nitrosourea-, ethylmethanesulphonate- and bleomycin-genotoxicity. Mutat Res 2006;607:225-30.

Garcia SB, Novelli M, Wright NA. The clonal origin and clonal evolution of epithelial tumours. Int J Exp Pathol 2000;81:89-116.

Egner PA, Wang JB, Zhu YR, Zhang BC, Wu Y, Zhang QN, et al. Chlorophyllin intervention reduces aflatoxin-DNA adducts in individuals at high risk for liver cancer. Proc Nat Acad Sci U S A 2001;98:14601-6.

Fernández MJ, López A, Santa-Maria A. Apoptosis induced by different doses of caffeine on Chinese hamster ovary cells. J Appl Toxicol 2003;23:221-4.

He Z, Ma WY, Hashimoto T, Bode AM, Yang CS, Dong Z. Induction of apoptosis by caffeine is medicated by the p53, Bax, and caspase 3 pathway. Cancer Res 2003;63:4396-401.