To Study Bacteriological Profile and Antibiotic Sensitivity Pattern in Cases of Neonatal Sepsis

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  • ,IN
  • ,IN
  • ,IN
  • ,IN

Keywords:

Antibiotic Sensitivity, Bacteriological Profile, Gram Negative Organisms, Neonatal Sepsis.
Education and Research

Abstract

Objective: To study the bacteriological profile and sensitivity pattern in cases of Neonatal sepsis at 12 bedded Neonatal Intensive Care Unit (NICU). Methods: This was a prospective study of neonates admitted to our NICU from Jan 2010 to Oct 2011 with diagnosis of neonatal sepsis or those who developed sepsis later during their stay in NICU. All newborns diagnosed as a case of neonatal sepsis, based on clinical features with positive sepsis screen and/or positive blood culture, were included in our study. Blood Culture&Sensitivity was done with conventional non-automated method using Herley's Broth. Results: Out of 55 cases blood culture was positive in 27 (49.09%) cases. Klebsiella Pneumoniae 15 (55.55%) was the most common organism isolated in both early and late onset sepsis showed sensitivity to collistin in 86.6% of cases followed by sensitivity to imipenam-cilastin in 46.67% cases. Only 20% Klebsiella isolates were sensitive to drugs like Ampicillin-sulbactum, Amikacin, Tazobactum, cefpime and for other antibiotics like Meropenam, Piperacillin-tazobactum, Vancomycin it was less than 20%. Other organisms isolated were Staph. aureus, E. Coli, Pseudomonas, Enterobactor, Acinetobactor and candida species. Conclusion: Gram negative organisms are most common cause of early as well as late onset sepsis and there is alarming degree of antibiotic resistance to commonly used antibiotics.

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2015-06-01

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Original Research Article

 

References

Bang AT, Band RA, Bactule SB, Reddy HM, Deshmukh. Effect of home based neonatal care and management of sepsis on neonatal mortality: field trial in rural India. Lancet. 1999; 354:1955-61.

James L, Wynn, Leuy O. Role of Innate Host Defenses in Susceptibility to Early - Onset Neonatal Sepsis. Clin Perinatol. 2010; 37:307-37.

Sepsis, NNF teaching aids: Newborn care. Neonatal sepsis, Slide NS-4 Early vs late.

National Neonatal Perinatal Database. Report 2002-03. New Delhi: NNPD nodal center, Department of Pediatrics, All India Institute of Medical Sciences.

Daikoku NH, Kaltreider F, Johnson TR, et al. Premature rupture of membranes and preterm labour: neonatal infection and perinatal mortality risk. Obstet Gynecol. 1981; 58:417-25.

Escobar GJ. The neonatal "sepsis work-up” personal reflections on the development of a evidence based approach toward newborn infections in a managed care organization. Pediatrics. 1999; 103(1):360-73.

Shankar MJ, Agarwal MJ, Deorari AK, Paul VK. Sepsis in the Newborn. Indian J Pediatr. 2008; 75:261-6.

Remington JR, Klein JO. In Infectious diseases of the fetus and newborn infants. 5th ed. Philadelphia: WB Saunders; 2001.

Dear P. Infection of newborn. In: Rennie JM, editor. Textbook of Neonatology. 3rd ed. Edinburgh: Churchill Living stone; 1999. p. 1109-37.

Stoll BJ. Infections of the neonatal infant. In: Kliegman RM, Jenson HB, Behrman RE, Stanton BF, editors. Nelson textbook of Pediatrics. 18th ed. New Delhi: Saunders; 2008. p. 794-811.

Lee AC, Mullany LC, Tielsch JM, Katz J, Khatry SK, LeClerq SC, Adhikari RK, Shrestha SR, Darmstadt GL. Risk factors for neonatal mortality due to birth asphyxia in southern Nepal: a prospective, community-based cohort study. Pediatrics. 2008 May; 121(5):e1381-90.

Zaidi AKM, Huskins WC, Thaver D, Bhutta ZA, Abbas Z, Goldmann DA. Hospital-acquired neonatal infections in developing Countries. Lancet. 2005; 365:1175-88.

Tallur SS, Kasturi AV, Nadgir SD, Krishna BS. Clinico-bacteriological study of neonatal septicemia in Hubli. Indian J Pediatr. 2000; 67(3):169-74.

Joshi SG, Ghole VS, Niphadkar KB. Neonatal Gram-negative bacteremia. Indian J Pediatr. 2000; 67(1):27-32.

Kuruvilla KA, Pillai S, Jesudason M, Jana AK. Bacterial profile of sepsis in a neonatal unit in south India. Indian Pediatr. 1998; 35:851-8.

Ahmed NU, Chowdhury A, Hoque M, Darmstadt GL. Clinical and bacteriological profile of neonatal septicemia in a tertiary level pediatric hospital in Bangladesh. Indian Pediatr. 2002; 39:1034-8.

Deorari AK. Changing pattern of bacteriologic profile in neonatal sepsis among intramural babies. Journal Neonatology. 2006; 20(1):8-15.

Mutlu M, Aslan Y, Saygin B, Yilmaz G, Bayramoglu G, Koksal I. Neonatal sepsis caused by Gram-negative bacteria in Neonatal intensive care unit. HK J Paediatr. 2011; 16:253-7.

Movahedian AH, Moniri R, Mosayebi Z. Bacrerial culture of Neonatal sepsis. Iranian J Public Health. 2006; 35(4):84-9.