Analysis of Collagen Fibres in Human Dental Pulp using Picrosirius Red Stain and Polarised Microscopy

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Authors

  • Treville Pereira
     Department of Oral & Maxillofacial Pathology and Microbiology, Dr D. Y. Patil Dental College & Hospital, Sector 7, Nerul, Navi Mumbai, Maharashtra 400706 ,IN
  • Shashibhushan Dodal
     Department of Oral & Maxillofacial Pathology and Microbiology, Dr D. Y. Patil Dental College & Hospital, Sector 7, Nerul, Navi Mumbai, Maharashtra 400706 ,IN
  • Avinash Tamgadge
     Department of Oral & Maxillofacial Pathology and Microbiology, Dr D. Y. Patil Dental College & Hospital, Sector 7, Nerul, Navi Mumbai, Maharashtra 400706 ,IN

Keywords:

Collagen Fiber Thickness, Human Dental Pulp, Picrosirius Red, Polarization Colors
Oral & Maxillofacial Pathology

Abstract

Background: The extracellular matrix (ECM) performs a very important function in growth regulation, tissue differentiation and organization. Diseases of pulp are seen in all ages. Collagen, the main macromolecule of dental pulp, varies in its number, thickness and distribution with age. Hence a study was designed to identify and analyze the collagen fibers in human dental pulp on the basis of polarizing colors in various age groups.

Material and methods: A study was conducted using the pulp of 30 non-carious teeth from patients of various ages and one tooth germ. Picrosirius red stain was used to stain the collagen present in dental papilla, dental follicle and dental pulp and the thickness of the fibers was assessed on the basis of the polarizing colors.

Results: Collagen fibers in dental follicle stained red while they were not stained in papilla. Collagen fibers in the pulp of teeth of younger individuals stained green to greenish yellow, in middle aged individuals, it stained orangeeorange red while in older individuals, it stained red.

Conclusion: The results from the study show that with aging, thickness of collagen fibers increases and there is change in their arrangement and packaging indicating fibrosis.

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Published

2018-09-18

How to Cite

Pereira, T., Dodal, S., & Tamgadge, A. (2018). Analysis of Collagen Fibres in Human Dental Pulp using Picrosirius Red Stain and Polarised Microscopy. Journal of Pierre Fauchard Academy (India Section), 28(3), 73–77. Retrieved from http://www.informaticsjournals.com/index.php/jpfa/article/view/22273

Issue

Volume 28, Issue 3, September 2014

Section

Original Articles

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All the articles published in JPFA are distributed under a creative commons license. The journal allows the author(s) to hold the copyright of their work (all usages allowed except for commercial purpose).

 

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