Comparative Evaluation of Tissue Dissolution Efficacy of Azadirachta indica Irrigant and Sodium Hypochlorite – An in vitro Study


Affiliations

  • Vishnu Dental College, Department of Conservative Dentistry and Endodontics, Bhimavaram, Andhra Pradesh, 534202, India
  • Vishnu Dental College, Department of Conservative Dentistry and Endodontics, Bhimavaram, Andhra Pradesh, 534202

Abstract

Objectives: This study aimed to evaluate the tissue dissolution efficacy of Azadirachta indica (A. indica) irrigant and sodium hypochlorite. Materials and Methods: 40 bovine muscle tissue specimens of equal dimensions were used in the experiment. Based on tissue specimen treatment, they were divided into four test groups - 3%NaOCl (Group I), 7.5% A. indica irrigant (Group II), alternative use of 3%NaOCl and 7.5% A. indica irrigant (Group III) and control group as distilled water (Group IV). Tissue specimens were weighed before and after placing in test solutions at 2 different time intervals (30,60 minutes). Results: The results revealed that 3%NaOCl showed highest tissue dissolution followed by alternative use of 3%NaOCl. and A. indica irrigant, A. indica alone and distilled water (Group I >III>II>IV). The larger the duration of contact with irrigant better is the tissue dissolution. Conclusion: 7.5% A. indica irrigant alone does not have any tissue dissolving property but in combination it acts as a neutral solution neither increasing nor decreasing the capability of 3%NaOCl.

Keywords

Azadirachta indica, NaOCl, Smear Layer, Tissue Dissolution

Subject Discipline

Dentistry

Full Text:

References

Stojicic S, Zivkovic S, Qian W, Zhang H, Haapasalo M. Tissue dissolution by sodium hypochlorite: effect of concentration, temperature, agitation, and surfactant. J Endod. 2010;36(9):1558-62. PMid:20728727

Ballal NV, Mala K, Bhat KS. Effect of maleic acid and ethylenediaminetetraacetic acid on the dissolution of human pulp tissue – An in vitro study. Int Endod J. 2011;44:353– 356. PMid:21219363

Cobankara FK, Ozkan HB, Terlemez A. Comparison of organic tissue dissolution capacities of sodium hypochlorite and chlorine dioxide. J Endod. 2010;36(2):272–274. PMid:20113788

Aarati N, Ranganath NN, Soumya BG, Kishore B, Mithun K. Evaluation of antibacterial and anticandidal efficacy of aqueous and alcoholic extract of neem (Azadirachta indica) an invitro study. IJRAP. 2011;2(1):230–235.

Chhibber S, Sharma N. Medicinal and therapeutical potential of neem (Azadirachta indica). A Review International Journal of Scientific and Research Publications. 2014;4(5):1–5.

Dutta A, Kundabala M. Comparative anti-microbial efficacy of Azadirachta indica irrigant with standard endodontic irrigants: A preliminary study. J Conserv Dent. 2014;17(2):133–137. PMid:24778508. PMCid:PMC4001268

Tandan S, Chandra S, Gupta S, Tripathi H, Lal J. Pharmacological effects of Azadirachta indica leaves. Fitoterapia. 1990;LXI:75–78.

Irala LE, Grazziotin-Soares R, Salles AA, Munari AZ, Pereira JS. Dissolution of bovine pulp tissue in solutions consisting of varying NaOCl concentrations and combined with EDTA. Braz Oral Res. 2010;24(3):271–276. PMid:20877962

Taneja S, Mishra N, Malik S. Comparative evaluation of human pulp tissue dissolution by different concentrations of chlorine dioxide, calcium hypochlorite and sodium hypochlorite: An in vitro study. J Conserv Dent. 2014;17(6):541–545. PMid:25506141. PMCid:PMC4252927

Christensen CE, McNeal SF, Eleazer P. Effect of Lowering the pH of sodium hypochlorite on dissolving tissue in Vitro. J Endod 2008;34:449–452. . PMid:18358894

Moorer WR, Wesselink PR. Factors promoting the tissue dissolving capability of sodium hypochlorite. Int Endod J. 1982;15:187–196.

Rossi-Fedele G, Steier L, Dogramaci EJ, Canullo L, Steier G, de Figueiredo JA. Bovine pulp tissue dissolution ability of HealOzone, Aquatine Alpha Electrolyte and sodium hypochlorite. Aust Endod J. 2013;39:57–61. . PMid:23890260

Baker, John R. Principles of Biological Microtechnique. A Study of Fixation and Dyeing. London: Methuen & Co. Ltd; 1958.


Refbacks

  • There are currently no refbacks.