Journal of Natural Remedies

Molecular Basis of Sida cordifolia (L.) Induced Apoptosis in Melanoma Cell Line

2023-09-25T14:57:59+0530

Sida cordifolia of the family Malvaceae is widely used in traditional medicine for treating inflammation, respiratory and neurological ailments and wound healing. Its extract was found to possess effective antitumor activity in hepatocellular carcinoma and HeLa cell lines. This study was aimed at screening the anticancer activity of S. cordifolia and to investigate its mechanism of action. Aerial parts of the plant were subjected to hot continuous extraction by Soxhlet apparatus with ethanol as solvent. Cytotoxicity of the extract was assessed in various cancer cell lines viz. breast, ovarian, colon, skin, and liver cancer by MTT assay. For each cell line, the IC₅₀ value was calculated. The mechanism of anticancer activity of the extract was studied in melanoma cells by exposing them to 12.5 and 25 μg/ml extract and comparing results with the control. Gel electrophoresis was used to analyse DNA laddering. Expression of TP53, Bcl and Caspase gene family proteins were determined by SDS-PAGE. Mitochondrial membrane potential was studied by the JC-1 kit. Cell cycle analysis was performed by using a flow cytometer. Statistical analysis was done by ANNOVA, and significant values were further analysed by Tucky post-hoc analysis. P value less than 0.05 was considered statistically significant. MTT assay revealed maximum cytotoxicity of the extract against melanoma with an IC₅₀ value of 16.51μg/ml. Melanoma cells treated with the extract demonstrated dose-dependent DNA laddering. The extract also exhibited a dose-dependent increase in the level of Bax, Caspase 3, Caspase 9 and p53 proteins. Expression of Bcl2 protein was significantly reduced. Treatment of melanoma cells with the extract showed significant loss of mitochondrial membrane potential. Melanoma cell population in subG0 and G2/S was significantly elevated. From these results, we conclude that ethanol extract of S. cordifolia is cytotoxic to melanoma cells. It acts by inducing apoptosis via an intrinsic mechanism. The extract also arrests melanoma cells in the G2/M phase.

UFLC-MS Method Development of Vasicinone, Pellitorine, 6-Gingerol, Costunolide, Dehydrocostuslactone, Apigenin, and Validation of Piperine, Biflorin in Polyherbal Formulations

2023-09-25T13:20:41+0530

Standardisation of polyherbal formulations is necessary for the quality, safety, quantity, and efficacy of botanicals in marketed and newly established formulations. The Ministry of Ayush, Govt of India, recommended using polyherbal formulations to treat COVID-19, i.e., Kabusura kudineer, Nilavembu kudineer, etc., Kabusura kudineer is a Siddha-based formulation. It prevents and treats COVID-19 due to some botanicals in Kabusura kudineer, which have proven anti-inflammatory, anti-viral, and immunomodulatory effects. The current work focuses on establishing a standard protocol for the Kabusura kudineer marketed, a novel dosage form called Hydaljss08, and in plant species, mainly present in both formulations. Both formulations contain some similar crude drugs and their active constituents. They are Zingiber officinale rhizome, Syzygium aromaticum flower buds, Adhatoda vasica leaves, Anacyclus pyrethrum roots, Saussurea lappa roots, Piper longum fruits, Clerodendrum serratum roots, Coleus amboinicus roots, contain active phytopharmaceuticals are 6-gingerol, biflorin, vasicinone, pellitorine, costunolide, dehydrocostuslactone, piperine, and apigenin. Existing liquid chromatography methods were reported for individual above active compounds, but not in these formulations and combined dosage forms. Working UFLC methods have not been reported individually nor combined for the Biflorin. The current study aims to develop UFLC methods for 6-gingerol, biflorin, vasicinone, pellitorine, costunolide, dehydrocostuslactone, piperine, and apigenin in polyherbal formulations Kabusura kudineer marketed, Hydaljss08 and in isolated, fractions, extract of plant species present in both dosage forms. The preliminary identification of the phytopharmaceuticals in the polyherbal formulations, isolated fractions, and extract of plant species was done by TLC and IR spectrum. The developed liquid chromatography method was novel, simple, linear, and rapid for estimating 6-gingerol, biflorin, vasicinone, pellitorine, costunolide, dehydrocostuslactone, piperine, apigenin in a plant species, and Ayush-based formulations.

Is Jackfruit Holding the Key Answer to Male Infertility? Outcomes of a Preliminary Pre-clinical Study

2023-06-20T10:49:58+0530

Purpose: The antioxidant activity of jackfruit can be a potential treatment for male infertility. The objectives of the study were to evaluate the effect of jackfruit (Artocarpus heterophyllus) extract on testicular dysfunction and cytogenetic changes induced by Chlorpyrifos in male Wistar albino rats by using sperm analysis, histopathologic analysis of testis, biochemical analyses, and cytogenetic studies; and to determine if these were dose-dependent. Methods: Ethanolic extract of jackfruit (JFE) (450mg/kg, 600mg/kg) and Chlorpyrifos (CPF) (17.5mg/kg) were used. Thirty male rats grouped into control, toxic (CPF), jackfruit, treatment-1 and -2 received distilled water+corn oil, chlorpyrifos, JFE, CPF 17.5mg+JFE 450mg/kg, and CPF 17.5mg+JFE 600mg/kg, respectively. Network pharmacology was used to track, and identify the active ingredients and target pathways. Results: Treatment groups particularly treatment 1 displayed greater weight gain. JFE was associated with an increased testicular weight (p<0.05). Sperm motility significantly increased in the jackfruit, control, and treatment groups, particularly treatment 2. The increase in sperm count and motility were dose-dependent. There was a significant decrease in the DNA damage with JFE. There was an increase in the total protein in the treatment groups. Sperm motility, vitality, and total proteins are significantly reduced with CPF. There was a reduction in malondialdehyde (MDA) and an increase in catalase activity in the jackfruit treatment groups. Network analysis analysed 50 active compounds in JFE, and identified 12 potential targets. Conclusion: JFE has a positive impact on sperm count, motility, vitality, and chromatin integrity demonstrated by decreased DNA damage. Antioxidant assays, MDA, and catalase indicated strong antioxidant activity of JFE.

Safety and Prescription Trends of Rasa Aushadhis (Ayurveda Herbomineral Formulations): A Critical Appraisal of Reported Medical Practices from CCRAS Experience

2023-09-22T17:20:30+0530

Background: In Ayurveda, metal and mineral-based formulations are popularly practised for their pleasant applicability and first-rate potency. There is no doubt about the safety and potency of Ayurveda Rasa Aushadhis if they are prepared and used as per the terms mentioned in the literature. For the globalisation of Ayurveda, safety and efficacy with quality control have become important affairs for the scientific presentation of truth/reality. The present study was undertaken to document and analyse the information from clinical records about gross safety and prescription trends of different Ayurveda Rasa Aushadhis. Methodology: Primary data collection was performed from 24 clinical centres of CCRAS through a prescribed format. Prescription records of patients during the last five years (1^st April 2012 to 31^st March 2017); irrespective of age, sex and religion, were analysed. Evaluation of these clinical records confined to prescription trends and consumption of 15 commonly prescribed Rasa Aushadhis (herbs-mineral formulations) was done to infer. Result and Observation: Overall, 1,09,307 patients were treated with prescribed Rasa Aushadhis, and there were no Adverse Drug Reactions (ADR) or Suspected Adverse Reactions (SAR) or any untoward effects associated with the prescribed Rasa Aushadhis, during the full course of treatments as well as later/after follow-up. Analysis shows the conditions of clinical application of the Rasa Aushadhis were duly based on fundamental concepts of Ayurveda. Conclusion: Based on retrieved data from reported medical practices of Rasa Aushadhis in different clinical research facilities, the present study reveals the safety and rationale of using 15 commonly used Rasa Aushadhis across India. The study would certainly disseminate the merits of Ayurvedic Rasa Aushadhis in understanding gross safety and potency.

Phytochemical Screening of Diospyros paniculata Bark and In Vitro Cytotoxic Study on Human Breast Cancer Cell Line

2023-12-22T12:08:58+0530

Medicinal plants are the resource of traditional medicines and modern medicine is also developed indirectly from plants. In traditional medicinal practices such as Ayurveda and Unani, Diospyros paniculata has been recognised for its medicinal properties. Different parts of the plant, including the leaves, bark, fruits, and seeds, are utilised to prepare various herbal remedies and these species show a richness of triterpenoids, naphthoquinones and naphthaldehydes which majorly contribute to the anticancer activity. D. paniculata bark was extracted with methanol and fractionated by low-polar to high-polar solvents. In-vitro cytotoxic activity of each solvent fraction was studied on the MCF-7 (Human Adenocarcinoma) cell line by MTT assay. The ethyl acetate fraction shows a significant IC₅₀ value of 23.47 µg/ml which might be due to the presence of principal compounds in the fraction. Methanol fraction showed a moderate IC₅₀ value. To isolate chemicals, the chloroform fraction underwent column chromatography. Fourier Transform Infrared Spectroscopy (FTIR), ¹H NMR (Nuclear Magnetic Resonance Spectroscopy), and ¹³C NMR were used to characterise the isolated chemicals. Two compounds were isolated from chloroform fraction identified as Betulin and Lupeol.

In Vivo Hepatoprotective Activity of Hydroalcohol Extract of Gyrocarpus asiaticus Willd and Lactuca runcinata DC

2023-12-11T18:04:24+0530

Objective: Investigation of hepatoprotective activity of Gyrocarpus asiaticus Willd (GA) and Lactuca runcinata DC (LR) prepared by hydroalcohol extraction. Methodology: Albino rats were used for the in vivo experiments for the determination of oral acute toxicity study. For these experiments, 6 groups were created of which 5 groups were for each plant extract and 1 group for control. A total of 11 groups were made for the toxicity study. Each group required 2 albino mice. Different doses of 0.025, 0.2, 0.5, 2, and 5 gm/kg body weight were administered orally for each plant extract. For the control group, these were administered with distilled water. For hepatoprotective activity, albino rats were randomly divided into 5 (control, toxic, standard, 2 samples) groups, and 4 animals were randomly divided into each group. For 2 plants, a total of 7 groups were made. 5% gum acacia was used as the vehicle. For induced hepatotoxicity CCl₄ and as a standard drug silymarin was used. Result and Discussion: Plant extracts did not show any toxicity, and no histopathological changes were seen in the liver, kidney, or lungs due to toxicity. In the study of GA Willd and LR DC extract prepared by hydroalcohol solution, there was evidence of protection against CCl₄-induced hepatotoxicity in the Histopathological study of the liver of albino rats. Conclusion: Hydroalcohol extract of LR DC and GA Willd shows no oral acute toxicity and LR DC shows no significant hepatoprotective activity but GA Willd shows significant hepatoprotective activity

Preliminary Phytochemical Analysis, In-vitro Evaluation of Antibacterial and Anticancer Activity of Cleome aspera Aerial Part Extracts

2024-01-02T19:30:19+0530

Various species of the Cleome genus belong to the circle of relatives Cleomaceae family, and order Brassicales, constitute flowering flora with a long record of use in conventional remedies, and have been related to the prevention of many illnesses. Cleome aspera has not been thoroughly investigated in the medicinal area; however, it may offer a therapeutic choice in favour of the management of various disorders. In this recent study, the aerial parts of C. aspera were extracted with petroleum ether, ethyl acetate, and ethanol sequentially in ascending polarity order. Ethanol extract exhibited a rich phytochemical profile and antibacterial and anti-cancer action among the solvent extracts. The phytochemical analysis of three extracts exhibited the existence of many secondary metabolites like flavonoids, alkaloids tannins, polyphenols, steroids, glycosides, and terpenoids. The antibacterial activity against two Gram (+) bacteria, Staphylococcus aureus (ATCC 6538), and Bacillus subtilis (ATCC 6633), as well as two Gram (-) bacteria, Pseudomonas aeruginosa (ATCC 9027) and Escherichia coli (ATCC 8739) was assessed at three concentrations (50, 100, 200 μg/ml) of each extract. The broth dilution method tested the extract’s minimum inhibitory concentration against four bacterial strains. Using the MTT (3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide) assay, the three extracts (petroleum ether, ethyl acetate, and ethanol) of C. aspera were evaluated for in-vitro cytotoxic activity. The extracts were detected to have substantial antibacterial activity against Gram-positive as well as Gram-negative bacteria in a dose-dependent manner. The ethanol extract of C. aspera showed notable anticancer efficacy after comparing it with petroleum ether, and ethyl acetate extracts. This study proved that the plant C. aspera has potential as a source of plant-based drugs and has therapeutic qualities like antibacterial and anticancer activities. It may provide a healing choice for the control of several diseases. A further extensive study on the isolation of active ingredients, purification, characterization, and elucidation of viable mechanisms can be executed.

Development and Characterization of Charantin Loaded Transferosomes based Transdermal Formulation: An Effective and Patient Friendly Way for Diabetes Management

2023-12-22T14:08:51+0530

Transferosomes, marvels of scientific ingenuity, represent exquisitely optimized and ultra- deformable (ultra-flexible) lipid supramolecular aggregates, uniquely capable of traversing the mammalian skin intact. These remarkable carrier systems have harnessed the power of transdermal delivery, enabling the transport of drugs, irrespective of their molecular weight from the tiniest compounds to the most substantial ones. The incorporation of Charantin into transferosomal gel formulation holds immense promise in advancing topical therapeutics. Charantin, a bioactive compound derived from Momordica charantia, possesses potent medicinal properties with antidiabetic, anti-inflammatory, antioxidant, and antimicrobial activities. When integrated into the lipid-based transferosomal system, Charantin's efficacy is significantly amplified due to enhanced skin penetration and improved stability against degradation. The versatile nature of transferosomal gels allows for encapsulating both hydrophilic and lipophilic compounds, making it an ideal carrier for Charantin regardless of its solubility characteristics. Driven by the pursuit of excellence, the present study sought to masterfully optimize the Transferosomes to orchestrate an unparalleled symphony of enhanced skin delivery for Charantin.

In Vitro Study of the Nutrients, Antioxidant, Glycemic Activities, and Storage Stability of the Instant Tamarind Seed Kernel Powder Soup Mix

2024-01-16T18:31:25+0530

This study aimed to develop a soup mix using roasted tamarind (Tamarindus indica L.) seed kernel powder and evaluate its physical, chemical, mineral, and sensory characteristics, as well as glycemic response, antioxidant activity, and shelf life. Four different formulations of Tamarind seed kernel powder Soup Mixes (TSM1-10%, TSM2-20%, TSM3-30%, and TSM4-40%) of tamarind seed kernel powder were used to replace corn flour, and a control group (100% corn flour) was also tested. The physicochemical, mineral, sensory characteristics, antioxidant activity, glycemic response, and shelf life of each soup mix were evaluated using standard procedures. TSM4 had the best physical properties, while chemical analysis showed that this formulation had the highest protein content (11.31%) and caloric value (404.99 Kcal/100g). TSM4 also had the highest mineral composition and the greatest levels of TFC (23.12mg/100g), TPC (14.08mg/100g), and DPPH activity (112.41%). All five soup mix formulations had low glycemic indices and low glycemic loads. Sensory analysis indicated that TSM3, which contained 30% tamarind seed kernel powder, was the most acceptable option, with an overall acceptability score of 8.04. Shelf-life evaluation showed that the Total Plate Count (TPC) and fungal growth increased significantly over time, but the soup mixes could be stored for up to 15 days. The study found that the prepared soup mixes were more cost-effective and affordable than commercial soup mixes available in the market. Additionally, the research explored the effect of processing steps on these compounds and innovation trends for developing healthier soups that cater to specific consumer requirements.

Errata Title: Isolation, Formulation and Assessment of Anti-inflammatory Properties of Ursolic Acid Nerium oleander

2024-03-19T17:36:19+0530

Exploring the Antimicrobial Efficacy of Panchavalkala Kwatha and Arka: A Comprehensive Study

2023-10-20T10:08:57+0530

Panchavalkala, a combination of five tree barks, is significant in Ayurvedic formulations as an individual ingredient and in composite preparations. These five barks are Vata (Ficus bengalensis L.), Udumbara (Ficus glomerata Roxb.), Ashwatha (Ficus religiosa L.), Parisha (Thespesia populanea Soland. ex-Correa.), and Plaksha (Ficus lacor Buch-Ham.). As per Ayurveda, Panchavalkala Kwatha has vrana ropana (wound healing) properties and is also known for its diverse properties encompassing antiseptic, anti-inflammatory, antioxidant, antibacterial, and antimicrobial attributes, rendering it valuable for wound purification and healing. This study aims to investigate the antimicrobial potential of Panchavalkala Kwatha (PVK) while validating its ancient Ayurvedic significance in wound healing. The study involves meticulously preparing Panchavalkala Kwatha and Panchavalkala Arka. Panchavalkala Kwatha is meticulously prepared by the guidelines stipulated in Sharangadhara Samhita, while Panchavalkala Arka (PVA) follows the principles outlined in Arka Prakasha. The antimicrobial activity of Panchavalkala Kwatha and Panchavalkala Arka was assessed using Escherichia coli and Staphylococcus aureus through the well-diffusion assay. Panchavalkala Kwatha exhibits a substantial zone of inhibition measuring 25 mm against E. coli and 28 mm against S. aureus. This antimicrobial efficacy is comparable to Ciprofloxacin’s, signifying potent antimicrobial activity. In contrast, Panchavalkala Arka shows no inhibitory effect on microbial growth. These results underscore the robust antibacterial activity of Panchavalkala Kwatha against both E. coli and S. aureus, while Panchavalkala Arka remains inert. This research furnishes primary evidence supporting the antibacterial properties of Panchavalkala Kwatha against E. coli and S. aureus. These findings suggest the potential utility of this Ayurvedic formulation in expediting wound healing processes.

An In Vitro Comparative Study of Antibacterial Activity of Calendula officinalis Mother Tincture and 30 Potency

2024-01-16T17:43:02+0530

In this research work, the antibacterial activity of Calendula officinalis was tested (in vitro) as opposed to the commonly encountered important bacterial strains, namely Staphylococcus aureus and Pseudomonas aeruginosa. For this research, the mother tincture and 30 potency of Calendula officinalis were used. The mother tincture and further potencies (in centesimal scale) were prepared with the help of absolute alcohol (99.6% v/v) following the guidelines given in the Homoeopathic Pharmacopoeia of India (HPI). The Minimum Inhibitory Concentration (MIC) was studied using the micro-broth dilution method, and the Zone of Inhibition (ZOI) was studied using the agar-well diffusion method. The results of the MIC and ZOI were then compared with Amoxicillin, which was used as the standard and finally the conclusions were drawn. The selected microorganisms were procured from the National Centre for Microbial Resources, Pune.

Venom Vanquish: A Comprehensive Review on Ethnobotany and Phytochemistry of Significant Traditional Medicinal Plants

2023-09-22T12:16:29+0530

Snakebite is a significant public health concern in many parts of the world, particularly in regions where venomous snakes are prevalent. Snakebites can range from mild to life-threatening, depending on the species of snake, the amount of venom injected, and the location of the bite. Globally, an estimated 5 million people are affected by snake bites each year, according to recent statistics. Since ancient times, traditional medicinal plants have been used to cure a wide range of illnesses, including snake bites. Secondary metabolites contained in plants were efficient in reducing the effects of snake venom. Snake venom is a potent toxin that can cause severe damage to the human body, affecting the nervous system, cardiovascular system, or both. Symptoms can range from mild swelling to life-threatening paralysis, respiratory failure, and hemorrhage. Enzyme activity is inhibited by flavonoids, alkaloids, and tannins, which additionally hinder blood from clotting and lessen inflammation. Treatments for snake bites that are more beneficial and less hazardous could result from the isolation and synthesis of natural drugs. With the use of cutting-edge scientific methods, we can maximize their potential and use their secondary metabolites to develop novel medications and therapies. The study of these plants and their potential uses in contemporary medicine requires more funding and investigation. This will advance our knowledge of these plants and help us create cures for some of the most lethal diseases.

Exploring the Therapeutic Potential of Caesalpinia bonducella for Male and Female Reproductive Health: A Comprehensive Review

2023-11-08T18:16:04+0530

This review explores the potential of Caesalpinia bonducella Linn., particularly its seeds, known as ‘bounducella’ or ‘little ball’, and its impact on diverse pharmacological activities. Its use in treating various illnesses, including male and female reproductive health, has been investigated. The data, gathered from reliable sources worldwide, transcend language barriers and align with Indian Ayurvedic and Siddha Pharmacopoeias. This article has compiled the pharmacognostic and phytochemical profiles of the seeds, emphasizing their influence on reproductive health, potentially aiding in PCOS management and addressing hyperandrogenism. Additionally, the seed extract of this plant has abortifacient and anti-fertility effects, suggesting that it is a potential alternative to Mifepristone. Nevertheless, further in vivo and in vitro research on seed constituents is essential for developing lead molecules for use in allopathic treatment.

Review on the Pharmacological Activities of Black Pepper

2024-01-12T18:16:52+0530

Black pepper (Piper nigrum (L.), Family: Piperaceae) which is considered as the king of spices is well known for its pungent odour and has gained a global consideration. Traditional uses of pepper include the treatment of piles, worms, diarrhoea, intermittent fever, cough, cold, dyspnea, and throat conditions. The bioactive components of pepper like pungent alkaloid piperine as well as its essential oil possess numerous pharmacological properties, including hypolipidemic, antibacterial, neuroprotective, anticancer, anticonvulsant, analgesic, with anti-inflammatory effects and so on. This review focuses on the research that has been carried out on black pepper’s chemical content, recent advancements in chemistry and its pharmacological effects.

Ethno-Medico-Botanical Studies of Plant Resources of Beed District, Maharashtra, India: Retrospect and Prospects

2024-01-09T10:18:52+0530

The Beed district of Maharashtra, India, harbors a rich repository of ethnobotanical knowledge regarding medicinal plants. This review delves into the traditional wisdom of local communities, documenting the therapeutic applications of 191 plant species belonging to 61 families. The study reveals a diverse array of medicinal remedies derived from various plant parts, with leaves being the most commonly utilized. Skin diseases, gastrointestinal disorders, respiratory infections, and fever emerged as the primary ailments treated with these plant-based remedies. This comprehensive review underscores the urgent need for further ethnobotanical exploration within the district to meticulously document the traditional knowledge associated with medicinal plants and rigorously validate their efficacy through scientific investigation. Delving into the secrets of these medicinal plants might unveil a wealth of novel herbal remedies, all while protecting these invaluable natural treasures.

The Power of Nature in Managing Diabetes: A Database of Ethno Indigenous Plants in India

2023-11-23T18:35:54+0530

The ethno anti-diabetic plant database of India is a comprehensive compilation of indigenous plants used in traditional medicine for the treatment of diabetes mellitus. This database contains information on 1305 plant species that have been reported to have anti-diabetic properties based on ethnobotanical surveys, scientific research, and traditional knowledge. The plants have been categorized according to their scientific name, family, local name, parts used and availability among the 29 States. The database aims to provide a valuable resource for researchers, healthcare professionals, and the general public to explore the potential of traditional medicine in the management of diabetes. It also highlights the need for further scientific investigations into the efficacy and safety of these plants for the development of new anti-diabetic drugs.

Enhancing the Properties of Natural Products and Other Drugs: Deuterium: A Novel Approach

2023-12-14T13:09:35+0530

Deuterium substitution is a new approach used to enhance the metabolic profile of a drug. The carbon-deuterium bond seems to be stronger than a usual carbon-hydrogen bond. It leads to improved biological half-life and prolonged action of the drug. The deuterated drugs also show improved pharmacokinetics of the drug and reduce the dosing frequency. This paves the way for drugs from natural sources with good therapeutic effects but poor pharmacokinetic profiles, which can be deuterated for improved properties. Though this seems to be an alternate pathway, the isotope exchange with hydrogen has to be studied well for toxicity and the safety profile of the drug should be evaluated. The present review provides an outline of the deuterium approach in natural products and other drugs which are opted for deuterium to improve the metabolic profile.

Herbal Solutions for Urinary Tract Infections: A Literature Review

2024-02-14T14:29:43+0530

If treatment is not received, Urinary Tract Infections (UTIs), which are frequent microbial illnesses affecting the urinary system, can lead to discomfort and problems. Because they have antibacterial qualities and can potentially reduce UTI symptoms, natural treatments have been used. Supplements or juice made from cranberries are one popular natural treatment. Proanthocyanidins, which are found in cranberries, have the potential to inhibit bacteria, especially E. coli, from adhering to the lining of the urinary tract, hence decreasing the risk of infection. Its efficacy is still up for discussion, though, and certain drinks’ high sugar content may make UTI symptoms worse. D-mannose, a kind of sugar included in fruits like peaches and cranberries, is another commonly utilised treatment. D-mannose inhibits germs from sticking to the walls of the urinary system, in a manner akin to that of cranberries. It’s believed to be effective against E. coli, a common UTI-causing bacterium, but it’s ideal dosage and long-term effects require further research. Probiotics are thought to be helpful for UTIs, particularly those that contain lactobacilli strains. They may lessen the chance of infection by assisting in the maintenance of a balanced population of bacteria in the urinary tract and gut. There is, however, little data to support their direct influence on UTI treatment. Herbal treatments with possible antibacterial effects, such as buchu, goldenseal, and bearberry (uva-ursi), have been used historically. These herbs have ingredients that may aid in the battle against germs in the urinary system, but further research is needed to determine how safe and effective they are. Increasing water consumption is a simple yet effective treatment. Drinking enough water dilutes urine, which lowers the quantity of bacteria that could lead to an illness, and aids in the removal of bacteria from the urinary system. Even while these natural treatments seem promising for treating UTIs, it is important to see a doctor before using them, particularly if you think the illness may be serious. Certain medical issues may prevent the use of natural therapies, or they may interact negatively with medicines. When used as the only treatment for acute UTIs, they are frequently less successful than when used as preventative measures or in conjunction with traditional medicines. The main line of treatment for severe or persistent UTIs is still medical intervention with antibiotics to avoid complications and recurrent infections.

Appropriate Timing of Medicine (Aushadhi Sevan Kal) Can Better Impact Patient Outcome — A Case Report of Acute Gastroenteritis (Atisara)

2023-11-09T13:41:41+0530

Aushadh sewan kal (ASK) is vital in managing the disease. ASK varies with various factors such as the disease’s strength, the patient’s strength, the predominance of Dosha (regulatory functional factors of the body), Dushya (which gets vitiated), etc. Precision in ASK is vital to achieve greater efficacy and a lesser quantity of medicine. Lack of this precision may produce unwanted effects. A 31-year-old female patient came to OPD of Kayachikitsa GAC, Nagpur, with complaints of loose motion (10 episodes), vomiting (4 episodes), nausea, abdominal pain, body aches, and general weakness since one day, headache, burning sensation in the stomach since morning. She was diagnosed with acute gastroenteritis (Atisara). Atisara treatment containing Musta, Takra, and Bilvavleh was used. After the modification of the same medicines in ASK, pain reduction and complete recovery were achieved. There was a significant decrease in subjective parameters after administering medicine in appropriate ASK. Significant improvements in Jaran Shakti score (Capacity to digest), Abhyawaran shakti score (capacity to eat), and Ruchi factor (appetite) were also noted. ASK is important in the administration of Aushadha (medicine). Negligence in ASK may decrease the efficacy of medications. This case report emphasises the relationship between Agni (metabolic power), gut microbiome, circadian rhythm, and ASK. This case report gives future direction for various studies to understand the interplay between Agni (metabolic energy), gut microbiome, circadian rhythm, ASK, and the effect of this interplay on patient outcome.

Management of Alopecia Areata Through Micro Needling Along with Cupping

2023-11-24T11:17:56+0530

Ayurveda considered Alopecia areata as a minor ailment but now in this era of presentation looks are very important. Everyone wants to be beautiful and attractive. Hairs and skin are the mirror of beauty. If these two things are healthy and beautiful then the person looks more beautiful. An 18-year-old boy came to OPD with patches of baldness over his scalp. He has taken ‘n’ no. of treatment modalities for Alopecia areata but the results were not mesmerizing. The patient was advised to do microneedling with cupping for his baldness. According to the Pull and Tug test and SALT criteria, the patient had a 40% area of baldness at the center. After obtaining consent, the procedure was done thrice in 15 days. Along with this, internal medication with local application of oil was going on. In Internal medication, Asthimajja pachak (herbal drug combination), Praval Panchamrit (sea-origin calcium supplement), and Krumikurthar rasa (herbs-mineral drug) were given. After 4 months, with 15 sitting of microneedling with cupping, the patient had normal and healthy hair growth. Micro-needling along with internal medication is the best, cost-effective, and less invasive solution for re-growing hairs over high-cost hair treatments and transplantation.