Effect of Thyponium flageliforme in Combination with Curcuma zedoaria and Phyllanthus niruri in Expression of Protein Estrogen Receptor and Caspase-9 on Breast Cancer Mice


  • Sultan Agung Islamic University, Department of Chemistry, Medical Faculty, Jawa Tengah, 50112, Indonesia
  • Sultan Agung Islamic University, Department of Anatomy, Medical Faculty, Jawa Tengah, 50112, Indonesia
  • Sultan Agung Islamic University, Department of Biochemistry, Semarang, Jawa Tengah, 50112, Indonesia


Introduction: Incidence of breast cancer is high and constitutes a significant cause of female mortality. One of breast cancer type is Estrogen and Progesteron Receptor (ER and PR) positive, therefore affected by estrogen and progesteron concentration. Treatment of Thyponium flageliforme in combination with Curcuma zedoaria, and Phyllanthus niruri (TCP) have been proven capable of inhibiting tumor cells proliferation. Objective: To evaluate the expression of protein estrogen receptor and caspase-9 in C3H mice were inoculated with adenocarcinoma cells. Methods: 18 mice were inoculated with one million cells of breast adenocarcinoma per mouse and assigned into three groups. TCP with dosage 85 and 125 mg were treated for 21 days to evaluate the expression of protein estrogen receptor and caspase-9 measured by immuno-histochemical staining. Results: Mann Whitney analysis demonstrated that expression of protein estrogen receptor and caspase-9 in TCP 125 was significant (p < 0.05). Conclusion: Expression of protein estrogen receptor was decreased and caspase-9 expression was increased by TCP administration.


Breast Cancer, Caspase9, Combination of Herbal Extracts, Estrogen Receptors

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