Antioxidant and Anti Aging Assays of Oryza sativa Extracts, Vanillin and Coumaric Acid

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  • Medical Research Center, Faculty of Medicine, Maranatha Christian University, Jl Prof Drg Surya Sumantri No 65 Bandung 40164, West Java
  • Medical Research Center, Faculty of Medicine, Maranatha Christian University, Jl Prof Drg Surya Sumantri No 65 Bandung 40164, West Java
  • Medical Research Center, Faculty of Medicine, Maranatha Christian University, Jl Prof Drg Surya Sumantri No 65 Bandung 40164, West Java
  • Aretha Medika Utama, Biomolecular and Biomedical Research Center, Jl Babakan Jeruk 2, No 9, Bandung 40163, West Java
  • Aretha Medika Utama, Biomolecular and Biomedical Research Center, Jl Babakan Jeruk 2, No 9, Bandung 40163, West Java
  • Aretha Medika Utama, Biomolecular and Biomedical Research Center, Jl Babakan Jeruk 2, No 9, Bandung 40163, West Java
  • Aretha Medika Utama, Biomolecular and Biomedical Research Center, Jl Babakan Jeruk 2, No 9, Bandung 40163, West Java
  • Aretha Medika Utama, Biomolecular and Biomedical Research Center, Jl Babakan Jeruk 2, No 9, Bandung 40163, West Java
  • Aretha Medika Utama, Biomolecular and Biomedical Research Center, Jl Babakan Jeruk 2, No 9, Bandung 40163, West Java



Antioxidant, Anti aging, Coumaric Acid, Oryza sativa, Vanillin


Aging is a natural process in humans as accumulation of oxygen-derived free radicals which leads to the activation of hyaluronidase, collagenase and elastase, that can further contribute to cellular and tissue damage. Bioactive compounds from plants have been used as antioxidant that might inhibit aging processes as well. This study aimed to determine antioxidant and anti aging properties of Oryza sativa Extract (OSE), and its compounds, vanillin and coumaric acid. The phytochemical analysis of OSE was performed with Farnsworth modified method. Antioxidant activities were performed by measurement of 2,2-diphenyl 1-pichylhydazyl (DPPH) free radical scavenger, Ferric Reducing Antioxidant Power (FRAP), and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) reducing activity, while anti aging assay were observed through inhibitory of elastase, collagenase, and hyaluronidase activities. Phytochemical analysis showed the presence of terpenoids and saponins in high level. OSE showed lowest DPPH activity (IC50 = 314.51 μg/mL) compared to vanillin (IC50 = 283 μg/mL) and coumaric acid (IC50 = 255.69 μg/mL). In ABTS assay, OSE resulted lowest activity(IC50 = 145.67 μg/mL), compared to vanillin (IC50 = 4.96 μg/mL) and coumaric acid (IC50 = 1.67 μg/mL). OSE also showed the lowest FRAP-reducing activity (21.26 μM Fe(II)/μg), compared to vanillin (35.05 μM Fe(II)/μg) and coumaric acid (48.52μM Fe(II)/μg). OSE showed the lowest collagenase, elastase, and hyaluronidase inhibitory activity (IC50 = 816.78,107.51, and 203.13 μg/mL), compared to vanillin (IC50 = 16.27, 14.46, 45.23 μg/mL respectively) and coumaric acid (IC50 = 146.89, 25.38, 8.21 μg/mL respectively). In summary, OSE possess the lowest antioxidant and anti aging activities compared to vanillin and coumaric acid.


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Rattan SIS, Sodagam L. Gerontomodulatory and youth-preserving effects of zeatin on human skin fibroblasts undergoing aging in vitro. Rejuvenation Res. 2005; 8(1):46–57.

Kim DW, Hwang I, Kim D. Coenzyme Q10 effects on manganese superoxide dismutase and glutathione peroxidase in the hairless mouse skin induced by ultraviolet b irradiation. Bio Factors. 2007; 30(3):139–47.

Berneburg M, Plattenberg H, Medve-Konig K, Pfahlberg A, Gers-Barlag H, Gefeller O, et al. Induction of the photoaging-associated mitochondrial common deletion in vivo in normal human skin. J Invest Dermatol. 2004; 122(5):1277–83.

Yaar M, Eller MS, Gilchrest BA. Fifty years of skin aging. J Investig Dermatol Symp Proc. 2002; 7(1):51–8.

Demina N, Lysenko S. Collagenolytic enzymes synthesized by microorganisms. Mikrobiologiia. 1996; 65:293–304.

Pogrel M, Lowe M, Stern R. Hyaluronan (hyaluronic acid) in human saliva. Arch Oral Biol. 1996; 41(7):667–71.

Thring TS, Hili P, Naughton DP. Anti-collagenase,anti-elastase and anti-oxidant activities of extracts from 21 plants. BMC Compl Alter Med. 2009; 9(27):1–11.

Longo V, Finch C. Evolutionary medicine: From dwarf model systems to healthy centenarians? Sci. 2003; 299:1342–6.

Makrantonaki E, Brink T, Zampeli V, Elewa R, Mlody B, Hossini A. Identification of biomarkers of human skin ageing in both genders. Wnt signalling- a label of skin ageing? PLos ONE. 2010; 7(11).

Ndlovu G, Fouche G, Tselanyane M, Cordier W, Steenkamp V. In vitro determination of the anti-aging potential of four Southern African medicinal plants. BMC Complement Atern Med. 2013; 13(304):1–7.

Yasui H, Sakurai H. Chemiluminescent detection and imaging of reactive oxygen species in live mouse skin exposed to UVA. Biochem Biophys Res Commun. 2000; 269(1):131–6.

Kim H, Yun J, Lee J, Hong H, Jeong J, Kim E, et al. SUMO1 attenuates stress-induced ROS generation by inhibiting NADPH oxidase 2. Biochem Biophys Res Commun. 2011; 410(3):555–62.

Mruka D, Silvestrini B, Mo My, Cheng C. Antioxidant superoxide dismutase- A review, its function, regulation in the testis, and role in male fertility. Contracept. 2002; 65:305–11.

Moini H, Packer L, Saris N. Antioxidant and prooxidant activities of alpha-lipoic acid and dihydrolipoic acid. Toxicol Appl Pharmacol. 2002; 182(1):84–90.

Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 2004; 74(17):2157–84.

Kaur C, Kapoor H. Anti-oxidant activity and total phenolic content of some Asian vegetables. Int J Food Sci Technol. 2002; 37(2):153–61.

Mira N, Barros R, Schiocchet M, Noldin J, Lanfer-Marquez U. Extraction, analysis and distribution of phenolic acids in pigmented and non-pigmented genotypes of rice (Oryza sativa L.). Clencia e Tecnologia de Alimentos. 2008; 28:994–1002.

Pourali O, Asghari FS, Yoshida H. Production of phenolic compounds from rice bran biomass under subcritical water conditions. Chem Eng J. 2010; 160(1):259–66.

Martins S, Mussatto S, Martinez-Avila G, Montanez-Saenz J, Aguilar C, Teixeira J. Bioactive phenolic compounds: production and extraction by solid-state fermentation. Biotechnol Adv. 2011; 29:365–73.

Yang HJ, Yue Q, Cao YC, Zhang DF, Wang JQ. Effects of crude feruloyl and acetyl esterase solutions of Neocallimastix sp. YQ1 and Anaeromyces sp. YQ3 isolated from holstein steers on hydrolysis of chinese wild rye grasshay, wheat bran, maize bran, wheat straw and corn stalks. Anim Feed Sci Technol. 2009; 154:218–27.

Kilic I, Yesiloglu Y. Spectroscopic studies on the antioxidant activity of p-coumaric acid. Biomol Spectrosc. 2013; 115:719–24.

Widowati W, Wijaya L, Wargasetia T, Bachtiar I, Yellianty Y, Laksmitawati D. Antioxidant, anticancer, and apoptosis-inducing effects of piper extracts in hela cells. J Exp Integr Med. 2013; 3(3):225–30.

Widowati W, Ratnawati H, Rusdi U, Winarno W, Imanuel V. Phytochemical assay and antiplatelet activity of fractions of velvet been seeds (Mucuna pruriens). HAYATI J Biosci. 2010; 17(2):85–90.

Bera T, Chatterjee K, Ghosh D. In vitro antioxidant properties of the hydro-methanol extract of the seeds of Switenia mahagoni (L.) Jacq. BGM. 2015; 7(1):18–24.

Adnyana I, Abuzaid A, Iskandar E, Kurniati N. Pancreatic lipase and a-amylase inhibitory potential of mangosteen (Garcinia mangostana Linn.) pericarp extract. Int J Med Res Health Sci. 2016; 5(1):23–8.

Widowati W, Maesaroh M, Fauziah N, Erawijantari PP, Sandra F. Free radical scavenging and a-/b-glucosidase inhibitory activities of rambutan (Nephelium lappaceum L.) peel extract. Indones Biomed J. 2015; 7(3):157–62.

Sohn D, Kim Y, Oh S, Park E, Li X, Lee B. Hepatoprotective and free radical scavenging effects of Nelumbo nucifera. Phytomed. 2003; 10(2-3):165–9.

Widowati W. Green tea extract protects endothelial progenitor cells from oxidative insult through reduction of intracellular reactive oxygen species activity. J Basic Med Sci. 2014; 17(9):702–9.

Etoundi C, Kuat D, Ngondi J, Oben J. Anti-amylase Anti-lipase and antioxidant effects of aqueous extracts of some cameroonian spices. J Nat Products. 2010; 3:165–71.

Mishra A, Bapat M, Tilak J, Devasagayam T. Antioxidant activity of Garcinia indica (kokam) and its syrup. Curr Sci. 2006; 91:90–3.

Tu P, Tawata S. Antioxidant, antiaging, and anti-melanogenic properties of the essential oil from two varieties of Alpinia zerumbet. Molecules. 2015; 20:16723–40.

Kumar S, Sandhir R, Ojha S. Evaluation of antioxidant activity and total phenol in different varieties of Lantana camara leaves. BMC Res Notes. 2014; 7(560):1–9.

Widowati W, Ratnawati H, Husin W, Maesaroh M. Antioxidant properties of spice extracts. Biomed Eng. 2015; 1(1):24–9.

Miller NJ, Rice-Evans CA. Factors influencing the antioxidant activity determined by the ABTS+ radical cation assay. Free Radical Res. 1997; 26:195–9.

Antolovich M, Prenzler PD, Patsalides E, McDonald S, Robards K. Methods for testing antioxidant activity. Analyst. 2002; 127:183–98.

Sumantran VN, Kulkarni AA, Harsulkar A, Wele A, Koppikar SJ, Chandwaskar R, et al. Hyaluronidase and collagenase inhibitory activities of the herbal formulation Triphala guggulu. J Biosci. 2007; 32(4):755–61.

Tung JS, Mark GE, Hollis GF. A microplate assay for hyaluronidase and hyaluronidase inhibitors. Anal Biochem. 1994; 223:149–52.

Garodia P, Ichikawa H, Malani N, Sethi G, Aggarwal BB. From ancient medicine to modern medicine: Ayurvedic concepts of health and their role in inflammation and cancer. J Soc Integr Oncol. 2007 Mar 21;5(1):25–37.

Iqbal S, Bhanger M, Anwar F. Antioxidant properties and components of some commercially available varieties of rice bran in Pakistan. Food Chem. 2005; 93:265–72.

Cho JG, Huh J, Jeong RH, Baek NI. Inhibition effect of phenyl compounds from the Oryza sativa roots on melanin production in mMne B16-F10 melanoma cells. Nat Prod Res. 2014; 29(11):1–3.

Oliveira M, Feddern V, Kupski L, Cipolatti E, Badiale-Furlong E, Souza-Soares L. Changes in lipid, fatty acids and phospolipids composition of whole rice bran after solid-state fungal fermentation. Bioresour Technol. 2011; 102:8335–8.

Awika J, Rooney L. Sorghum phytochemicals and their potential impact on human health. Phytochem. 2004; 65:1199–221.

Dykes L, Rooney L. Sorghum and millet phenols and antioxidants. J Cer Sci. 2006; 44:236–51.

Quan T, Qin Z, Xia W, Shao Y, Voorhees J, Fisher GJ. Matrix-degrading metalloproteinases in photoaging. J Investig. 2009; 14(1):20–4.

Zubair M, Anwar F, Shahid SA. Effect of extraction solvents on phenolics and antioxidant activity of selected varieties of pakistani rice (Oryza sativa). Int J Agric Biol. 2012; 14:935–40.

Roginsky V, Lissi EA. Review of methods to determine chain breaking antioxidant activity in food. Food Chem. 2005; 92:235–54.

Ferguson L, Zhu S, Harris P. Antioxidant and antigenotoxic effects of plant cell wall hydroxycinnamic acids in cultured HT-29 cells. Mol Nutr Food Res. 2005; 49:585–93.

Svobodova A, Psotova J, Walterova D. Natural phenolics in the prevention ov UV-induced skin damage. Biomed Papers. 2003; 147(2):137–45.

Anwar F, Bhanger M, Kazi T. Relationships of rancimat and AOM values at varying temperatures for several oils and fats. J Am Oil Chem Sci. 2003; 80:151–5.

Chung HS, Shin JC. Characterization of antioxidant alkaloids and phenolic acids from anthocyanin-pigmented rice (Oryza sativa cv. Heugjinjubyeo). Food Chem. 2007; 104:1670–7.

Karimi E, Mehrabanjoubani P, Keshavarzian M, Oskouelan E, Jaafar HZ, Abdolzadeh A. Identification and quantification of phenolic and flavonoid components in straw and seed husk of some rice varieties (Oryza sativa L.) and their antioxidant properties. J Sci Food Agric. 2014; 94:2324–30.

Schmidt CG, Goncalves LM, Prietto L, Hackbart HS, Furlong EB. Antioxidant activity and enzyme inhibition of phenolic acids from fermented rice bran with fungus Rhizopus oryzae. Food Chem. 2014; 146:371–7.

Liyana-Pathirana CM, Shahidi F. Antioxidant properties of commercial soft and hard winter wheats (Triticum aestivium L.) and their milling fractions. J Sci Food Agric. 2006; 86:477–85.

Liu J, Wang C, Wang Z, Zhang C, Lu S, Liu J. The antioxidant and free-radical scavenging activities of extract and fractions from corn silk (Zea mays L.) and related flavone glycosides. Food Chem. 2011; 126:261–9.

Arya P, Kumar M. Chemoprevention by Triticum aestivum of mouse skin carcinogenesis induced by DMBA and croton oil-association with oxidative status. Asian Pacific J Cancer Prev. 2011; 12(12):143–8.

Smith B. Generalization of spatially variant apodization to nonintegral nyquist sampling rats. IEEE Trans Image Process. 2000; 9:1088–93.

Hooda R. Antiwrinkle herbal drugs-an update. J Pharmacog Phytochem. 2015; 4(4):277–81.

Shehada A. A review on natural bioactive compounds as potential antiwrinkle agents. World J Pharm. 2014; 3:528–44.

Mukherjee P, Maity N, Nema N, Sarkar B. Bioactive compounds from natural resources against skin aging. Phytomed. 2011; 19:64–73.

Santa-Maria C, Revilla E, Miramontes E, Bautista J, Garcinia-Martinez A, Romero E, et al. Protection against free radicals (UVB irradiation) of a water-soluble enzymatic extract from rice bran. study using human keratinocyte monolayer and reconstructed human epidermis. Food Chem Toxicol. 2010; 48(1):83–8.

Ludwig P, Bennets S, Gruber J. Rice meristem stimulates epigenetic rejuvenation: New technologies. S Afr Pharm Cosmet Rev. 2011; 38:34–7.

Mitra R. Medicinal plants of Indonesia. Asia Pac Biotech News. 2007; 11:727–35.

Liang JA, Wu SL, Lo HY, Hsiang CY, Ho TY. Vanillin inhibits matrix metalloproteinase-9 expression through down-regulation of nuclear factor-kb signaling pathway in human hepatocellular carcinoma cells. Mol Pharmacol. 2009; 75(1):151–7.

Jenkins G. Molecular mechanisms of skin ageing. Mech Ageing Dev. 2002; 123:801–810.

Siedle B, Hrenn A, Merfort I. Natural compounds as inhibitors of human neutrophil elastase. Planta Med. 2007; 73:401–20.

Azmi N, Hashim P, Hashim DM, Halimoon N, Nik Majid NM. Anti-elastase, anti-tyrosinase and matrix metalloproteinase-1 inhibitory activity of earthworm extracts as potential new anti-aging agent. Asian Pac J Trop Biomed. 2014; 4(1):348–52.

Hrenn A, Steinbrecher T, Labahn A, Schwager J , Schempp C, Merfort I. Plant phenolics inhibit neutrophil elastase. Planta Med. 2006; 72:1127–31.

Kanashiro A, Souza J, Kabeya L, Azzolini A, Lucisano-Valim Y. Elastase release by stimulated neutrophils inhibited by flavonoids: Importance of the catechol group. Z Naturforsch C. 2007; 62(5-6):357–61.

Piwowarski J, Kiss AK, Kozlowska-Wojciechowska M. Anti-hyaluronidase and anti-elastase activity screening of tannin-rich plant material used in traditional polish medicine for external treatment of diseases with inflammatory background. J Ethnopharmacol. 2011; 137:937–41.

Girish K, Kemparaju K, Nagaraju S, Vishwanath B. Hyaluronidase inhibitors: A biological and therapeutic perspective. Curr Med Chem. 2009; 16:2261–88.

Girish K, Kemparaju K. The Magic Glue Hyaluronan its Eraser Hyaluronidase: A biological overview. Sci. 2007; 80:1921–43.




How to Cite

Widowati, W., Fauziah, N., Herdiman, H., Afni, M., Afifah, E., Kusuma, H. S. W., Nufus, H., Arumwardana, S., & Rihibiha, D. D. (2016). Antioxidant and Anti Aging Assays of <I>Oryza sativa</I> Extracts, Vanillin and Coumaric Acid. Journal of Natural Remedies, 16(3), 88–99.



Received 2016-07-22
Accepted 2016-09-30
Published 2016-11-16

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