Development and Validation of Analytical Method for Estimation of Calcium in Herbomineral Formulation by Atomic Absorption Spectrophotometry

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Authors

  • Pinkal H. Patel
     Faculty of Pharmacy, Parul Institute of Pharmacy and Research, Parul University, Limda, Waghodia, Vadodara, Gujarat ,IN
  • Jaimin R. Shah
     Faculty of Pharmacy, Parul Institute of Pharmacy and Research, Parul University, Limda, Waghodia, Vadodara, Gujarat ,IN
  • Hardik Soni
     Vasu Health Care, 967/4, G.I.D.C road, G.I, D.C, Makarpura, Vadodara, Gujarat ,IN
  • Vishal Patel
     Vasu Health Care, 967/4, G.I.D.C road, G.I, D.C, Makarpura, Vadodara, Gujarat ,IN
  • Janki Patel
     Faculty of Pharmacy, Parul Institute of Pharmacy and Research, Parul University, Limda, Waghodia, Vadodara, Gujarat ,IN

DOI:

https://doi.org/10.18311/jnr/2022/31332

Keywords:

AAS, Bhasma, Complexometric Titration, Maxcal-C, Wet Digestion

Abstract

Calcium determination is normally done by conventional titrimetric methods (complexometric titration). In this study, a simple, precise, accurate, rapid and sensitive method has been developed for calcium (Ca) determination in Maxcal-C Tablet (herbo-mineral formulation) and its raw materials by Atomic Absorption Spectrophotometer (AAS). For complete digestion of the sample, closed vessel wet digestion technique was used with 69% HNO3 and 30% H2O2 (7:1) as digestive solvents. Analysis was performed on AAS (AA-6300) at λmax 422.7 nm. Calcium nitrate Ca(NO3)2 was used as a standard for Calcium and the linearity of the standard was excellent over a concentration range of 0.5 to 20 μgmL-1. The regression coefficient (r2) was 0.999. For intraday and interday precision % RSD was obtained between 0.48%-1.95% and 3.48%-5.01%, respectively which complied with the standard range for AAS. Recovery was achieved at 93.24%, 99.73%, 101.75% for 3 levels of spikes (80%, 100%, 120%) respectively, which complied with the standard range 75- 125% for AAS. However, the AAS method produced accurate and reproducible results and also overcame the colour and other ions interference, which are common problems with the titrimetric method. The results of both techniques (the AAS method and the titrimetric method) were compared, which showed good estimation by AAS.

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Published

2022-12-16

How to Cite

Patel, P. H., Shah, J. R., Soni, H., Patel, V., & Patel, J. (2022). Development and Validation of Analytical Method for Estimation of Calcium in Herbomineral Formulation by Atomic Absorption Spectrophotometry. Journal of Natural Remedies, 22(4), 597–606. https://doi.org/10.18311/jnr/2022/31332

Issue

Volume 22, Issue 4, October 2022

Section

Research Articles

License

Copyright (c) 2022 Pinkal Patel, Jaimin R. Shah, Hardik Soni, Vishal Patel, Janki Patel (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Europe PMC
Received 2022-09-26
Accepted 2022-12-15
Published 2022-12-16

 

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