Preparation and Characterization of Bovine Serum Albumin Nanoparticles of Curcumin using a Chemometric Approach

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Authors

  • Ayesha Syed
     Department of PharmaceuticsAl-Ameen College of PharmacyBengaluru ,IN
  • Preeti Karwa
     Department of Pharmaceutics, Al-Ameen College of Pharmacy, Near Lalbagh Main Gate, Hosur Road, Bangalore - 560027, Karnataka ,IN
  • V. Kusum Devi
     Department of Pharmaceutics, Nitte College of Pharmaceutical Sciences, Yelahanka, Bengaluru-560064 ,IN

DOI:

https://doi.org/10.18311/jnr/2022/30392

Keywords:

Bovine Serum Albumin Nanoparticles, Box Behnken Design, Curcumin, EPR Effect

Abstract

Bovine Serum Albumin (BSA) has been presupposed to be a versatile protein polymer for targeted drug delivery. BSA nanoparticles can lead to passive targeting of drugs to the inflamed joint via the Enhanced Permeability and Retention (EPR) effect and due to their specific affinity towards the inflamed joint because of the inadequacy of protein in the affected region. Hence, the aim of the study was to develop BSA nanoparticles loading curcumin (BSA_CUR_NPs) by nanoparticle albuminbound technology and its optimization was conducted by 33 Box Behnken Design (BBD) in order to achieve the desired particle size and entrapment efficiency. Further, the optimised nanoparticles were also assessed for polydispersity index, zeta potential, total drug content, and in-vitro drug release study. The response surface plots and equations generated by BBD predicted the relationship between variables under study. The optimised formulation C12 was found to have a particle size of 207.1 ± 1.36nm, PDI of 0.138 ± 0.03, entrapment efficiency of 75.04 ± 0.06 %, total drug content of 91.40 ± 0.08% and zeta potential of -32.9mV. The optimised nanoparticles exhibited good sustained release for up to 8 days. The use of a chemometric approach led to the development of BSA_CUR_NPs with the desired characteristics with a less experimental procedure. Therefore, it presents an important model for producing the nanoparticles of the desired characteristics using albumin as a polymer for the enhanced and sustained delivery of loaded drugs to the inflamed joint.

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Published

2022-12-16

How to Cite

Syed, A., Karwa, P., & Devi, V. K. (2022). Preparation and Characterization of Bovine Serum Albumin Nanoparticles of Curcumin using a Chemometric Approach. Journal of Natural Remedies, 22(4), 637–648. https://doi.org/10.18311/jnr/2022/30392

Issue

Volume 22, Issue 4, October 2022

Section

Research Articles

License

Copyright (c) 2022 Ayesha Syed, PREETI KARWA, V. KUSUM DEVI

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Europe PMC
Received 2022-06-06
Accepted 2022-08-03
Published 2022-12-16

 

References

Cukierman E, Khan DR. The benefits and challenges associated with the use of drug delivery systems in cancer therapy. Biochem Pharmacol. 2010; 80(5):762-70. https:// doi.org/10.1016/j.bcp.2010.04.020 DOI: https://doi.org/10.1016/j.bcp.2010.04.020

V. Sainz, J. Conniot, A. I. Matos, C. Peres, E. Zupancic, L. Moura, et al. Regulatory aspects on nanomedicines. Biochem. Biophys. Res. Commun. 2015; 468(3):504-10. https://doi.org/10.1016/j.bbrc.2015.08.023 DOI: https://doi.org/10.1016/j.bbrc.2015.08.023

KM Tyner, P Zou, X Yang, H Zhang, CN Cruz and SL Lee. Product quality for nanomaterials: Current U.S. experience and perspective. Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol. 2015; 7:640-54. https://doi.org/10.1002/ wnan.1338 DOI: https://doi.org/10.1002/wnan.1338

K. Ren, A. Dusad, R. Dong, et al., Albumin as a delivery carrier for rheumatoid arthritis. J. Nanomed. Nanotechol. 2013; 4:176. DOI: https://doi.org/10.4172/2157-7439.1000176

Neumann E, Frei E, Funk D, Becker M, Schrenk H, Muller- Ladner U and Fiehn, C.vNative albumin for targeted drug delivery. Expert Opin. Drug Deliv. 2010; 7(8):915-25. https://doi.org/10.1517/17425247.2010.498474 DOI: https://doi.org/10.1517/17425247.2010.498474

Wizard G M, Henson S, Hoots D, Robbins S, Van G. Comparative Study of Curcumin and Diclofenac Sodium in Rheumatoid Arthritis Patients. HerbClip. 2012.

Anand P, Kunnumakkara AB, Newman RA, Aggarwal BB. Bioavailability of curcumin: problems and promises. Mol Pharm. 2007; 4(6):807-818. https://doi.org/10.1021/ mp700113r DOI: https://doi.org/10.1021/mp700113r

Q Fu, J Sun, W Zhang, X Sui, Z Yan, Z He. Nanoparticle albumin-bound (NAB) technology is a promising method for anti-cancer drug delivery. Recent Pat. Anticancer. Drug Discov. 2009; 4:262-72. https://doi. org/10.2174/157489209789206869 DOI: https://doi.org/10.2174/157489209789206869

Maurya DP, Sultana Y, Aqil M, Ali A. Formulation and optimization of rifampicin microparticles by Box-Behnken statistical design. Pharm Dev Technol. 2011; 17(6):1-10. 51. https://doi.org/10.3109/10837450.2011.572892 DOI: https://doi.org/10.3109/10837450.2011.572892

Chaudhary H, Kohli K, Amin S, Rathee P, Kumar V. Optimization and formulation design of gels of Diclofenac and Curcumin for transdermal drug delivery by Box-Behnken statistical design. J Pharm Sci. 2011; 100(2):580-593. https://doi.org/10.1002/jps.22292 DOI: https://doi.org/10.1002/jps.22292

Zhang J, He B, Qu W, Cui Z, Wang Y, Zhang H, et al. Preparation of the albumin nanoparticle system loaded with both paclitaxel and sorafenib and its evaluation in vitro and in vivo. J Microencapsul. 2011; 28(6):528-536. https:// doi.org/10.3109/02652048.2011.590614 DOI: https://doi.org/10.3109/02652048.2011.590614

Jithan A, Madhavi K, Madhavi M, Prabhakar K. Preparation and characterization of albumin nanoparticles encapsulating curcumin intended for the treatment of breast cancer. Int J Pharm Investi. 2011; 1(2):119-125. https://doi. org/10.4103/2230-973X.82432 DOI: https://doi.org/10.4103/2230-973X.82432

Zu Y, Zhang Y, Zhao X, Zhang Q, liu Y and Jiang R. Optimization of the preparation process of Vinblastine Sulfate (VBLS)-loaded folate-conjugated bovine serum albumin (BSA) nanoparticles for tumor-targeted drug delivery using response surface methodology (RSM). Int J Nanomedicine. 2009; 4:321-333. https://doi.org/10.2147/ IJN.S8501 DOI: https://doi.org/10.2147/IJN.S8501

Dash S, Murthy PN, Nath L, Chowdhury P. Kinetic modeling on drug release from controlled drug delivery systems. Acta Pol Pharm. 2010; 67(3):217‐223.

Li F, Su H, Wang J, Liu J, Zhu Q, Fei Y, et al. Preparation and characterization of sodium ferulate entrapped bovine serum albumin nanoparticles for liver targeting. Int. J. Pharm. 2008; 349(1-2):274-282. https://doi.org/10.1016/j. ijpharm.2007.08.001 DOI: https://doi.org/10.1016/j.ijpharm.2007.08.001

Kolluru L, Rizvi S, D’Souza M, D’Souza M. Formulation development of albumin based theragnostic nanoparticles as a potential delivery system for tumor targeting. J Drug Target. 2012; 21(1):77-86. https://doi.org/10.3109/10611 86X.2012.729214 DOI: https://doi.org/10.3109/1061186X.2012.729214

Tarhini M, Benlyamani I, Hamdani S, Agusti G, Fessi H, Greige-Gerges H, et al. Protein-Based Nanoparticle Preparation via Nanoprecipitation Method. Materials. 2018; 11(3):394. https://doi.org/10.3390/ma11030394 DOI: https://doi.org/10.3390/ma11030394

Jafari SM, Assadpoor E, He Y, Bhandari B. Re-coalescence of emulsion droplets during high-energy emulsification. Food Hydrocoll. 2008; 22:1191-1202. https://doi.org/10.1016/j. foodhyd.2007.09.006 DOI: https://doi.org/10.1016/j.foodhyd.2007.09.006

McClements DJ. Food Emulsions: Principles, Practice and Techniques. Boca Raton, FL, USA: CRC Press; 1999.

Anarjan N, Jafarizadeh-Malmiri H, Nehdi IA, Sbihi HM, Al-Resayes SI, Tan CP. Effects of homogenization process parameters on physicochemical properties of astaxanthin nanodispersions prepared using a solvent-diffusion technique. Int J Nanomedicine. 2015; 10:1109-18. https://doi. org/10.2147/IJN.S72835 DOI: https://doi.org/10.2147/IJN.S72835