Comparative Analysis of Bioactive Compounds and Anticancerous Activities in Leaf and Stem Extract of Physalis minima


Affiliations

  • Bannari Amman Institute of Technology, Molecular Diagnostics and Biomolecular Characterization Laboratory, Sathyamangalam, Tamil Nadu, 638401, India
  • Bannari Amman Institute of Technology, Molecular Diagnostics and Biomolecular Characterization Laboratory, Sathyamangalam, Tamil Nadu, India

Abstract

Physalis minima, commonly known as native gooseberry is a perennial herb which grows mostly in the tropical regions and is used in the traditional medicines as a curative for gastric ulcers and also for respiratory tract related ailments. The leaf and stem crude extract was separated by soxhelet using chloroform as solvent followed by the phytochemical screening to identify the presence of secondary metabolites. The Fourier-transform infrared spectroscopy (FTIR) showed the presence of various functional group such as phenol, alkane, aldehyde, alkyls, amino acid, aromatic amino acids and secondary alcohol. The Gas chromatography - mass spectrometry (GC-MS) result showed the presence of total of 24 bioactive compounds in both extracts such as Heptacosane, 1-chloro, Heptacosane, Octadecanoic acid, N-hexadecanoic acid and Hexatriacontane. The extract was subjected to for its anti-cancerous activity using MTT assay which had a good result against HL60 cell line and antibacterial activities.

Keywords

HL60, MTT Assay, Physalis minima, Phytochemical Screening, Soxhlet Extraction

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