Comparative Analysis of Bioactive Compounds and Anticancerous Activities in Leaf and Stem Extract of Physalis minima


  • Bannari Amman Institute of Technology, Molecular Diagnostics and Biomolecular Characterization Laboratory, Sathyamangalam, Tamil Nadu, 638401, India
  • Bannari Amman Institute of Technology, Molecular Diagnostics and Biomolecular Characterization Laboratory, Sathyamangalam, Tamil Nadu, India


Physalis minima, commonly known as native gooseberry is a perennial herb which grows mostly in the tropical regions and is used in the traditional medicines as a curative for gastric ulcers and also for respiratory tract related ailments. The leaf and stem crude extract was separated by soxhelet using chloroform as solvent followed by the phytochemical screening to identify the presence of secondary metabolites. The Fourier-transform infrared spectroscopy (FTIR) showed the presence of various functional group such as phenol, alkane, aldehyde, alkyls, amino acid, aromatic amino acids and secondary alcohol. The Gas chromatography - mass spectrometry (GC-MS) result showed the presence of total of 24 bioactive compounds in both extracts such as Heptacosane, 1-chloro, Heptacosane, Octadecanoic acid, N-hexadecanoic acid and Hexatriacontane. The extract was subjected to for its anti-cancerous activity using MTT assay which had a good result against HL60 cell line and antibacterial activities.


HL60, MTT Assay, Physalis minima, Phytochemical Screening, Soxhlet Extraction

Full Text:


Mohana K, Purushothaman KK. Antifertility properties of Physalis minima. Bulletin of Medico-Ethno-Botanical Research. 1981; 2:135–43.

Singh S, Ehana NM, Dhar MM. Solaplumbin: An anticancer glycoside from Nicotiana plumbaginifolia. Phytochemistry, 1974; 13:2020–2.

Okwu DE, Josaiah C. Evaluation of the chemical composition of two Nigerian medicinal plants. African Journal of Biotechnology. 2006; 5:357–61.

Dahanukar SA, Kulkarni RA, Rege NN. Pharmacology of medicinal plants and natural products. Indian Journal of Pharmacology. 2000; 32:S81–118

Nathiya M, Dorcus D. Preliminary phytochemical and antibacterial studies on Physalis minima Linn. International Journal of Current Science. 2012:24–30.

Parmar C, Kaushal MK. Physalis minima. In: Wild Fruits. New Delhi, India: Kalyani Publishers; 1982.

Tiger L. The natural guide to the medicinal herbs and plants (1st ed.), Tigerbooks, PLS, Twitchenhanze, UK; 1980. p. 12–15.

Karpagasundari C, Kulothungan S. Analysis of bioactive compounds in Physalis minima leaves using GC MS, HPLC, UV-VIS and FTIR techniques. Journal of Pharmacognosy and Phytochemistry. 2014; 3(4):196–201.

Vinson JA, Dabbagh YA, Serry MM, Jang J. Plant flavonoids, especially tea flavonoids are powerful antioxidant using an invitro antioxidant model for heart disease. Journal of Agricultural and Food Chemistry. 1995; 43:2800–2.

Agbor AG, Talla L, Ngogang JY. The Antidiarrhoeal activity of Alchornea cordifolia leaf extract. Phytotherapy Research: PTR. 2004; 18(11):873–6.

Chothani DL, Vaghasiya HU. A phyto-pharmacological overview on Physalis minima Linn. Indian Journal of Natural Products and Resources. 2012; 3:477–82.

Ramkumar KM, Rajaguru IP, Ananthan ZR. Antimicrobial properties and phytochemical constituents of an antidiabetic plant Gymnema montanum. IDOSI publications. Advances in Biological Research. 2007; 1(1–2):67–71.

Aqil F, Ahmed I, Mehmood Z. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turkish Journal of Biology. 2006; 30:177– 83.

Sumathy V, Lachumy SJ, Zakaria Z, Sasidharan S. In vitro bioactivity and phytochemical screening of Musa acuminata flower. Pharmacologyonline. 2011; 2:118–27.


  • There are currently no refbacks.