Qualitative and Quantitative Determination of Various Extracts of Ocimum basilicum L. Leaves


Affiliations

  • Periyar Maniammai Institute of Science and Technology, Department of Biotechnology, Thanjavur, Tamil Nadu, 613 403, India
  • Periyar Technology Business Incubator, TanBio R and D Solution, Thanjavur, Tamil Nadu, 613 403, India

Abstract

Ocimum basilicum L. (Lamiaceae) commonly known as basil possesses various medicinal activities to treat headache, diarrhoea, kidney malfunctions, diabeties, cancer and several pharmacological activities. These are mainly due to the various chemically diversified constituents present in it. In order to evaluate the chemical composition of basil leaves, the dried leaves were extracted with petroleum ether, chloroform, ethyl acetate, methanol and water by Soxhlet’s extraction. The extracts were screened for the qualitative determination by preliminary phytochemical screening. Quantitative determination was also performed for the total alkaloids, flavonoids, phenols, saponins and tannins. Amongst the extracts, methanol (MeOH) was identified to contain various classes of secondary metabolites, so GCMS analysis was performed on the MeOH extract. The results revealed that chemical constituents claimed for various biological activities were present.

Keywords

Chemical Constituents, GCMS, Ocimum basilicum L., Qualitative, Quantitative

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References

Ladwani AMA, Salman M, Abdel Hameed ES. Chemical composition of Ocimum basilicum L. essential oil from different regions in the Kingdom of Saudi Arabia by using Gas chromatography mass spectrometer. Journal of Medicinal Plants Studies. 2018; 6(1):14–19.

Nagarasan S, Boominathan M. Invitro studies on the primitive pharmacological activities of Adhatodavasica. International Journal of Life Sciences. 2016; 4(3):379–85.

Hussain AI, Anwar F, Hussain ST, Przybylski R. Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chemistry. 2008; 108(3):986–95. https:// doi.org/10.1016/j.foodchem.2007.12.010 PMid:26065762

Navarro V, Villarreal ML, Rojas G, Lozoya X. Antimicrobial evaluation of some plants used in Mexican traditional medicine for the treatment of infectious diseases. Journal of Ethnopharmacology. 1996; 53(3):143–47. PMid: 8887021. https://doi.org/10.1016/0378-8741(96)01429-8

Purushothaman B, PrasannaSrinivasan R, Suganthi P, Ranganathan B, Gimbun J, Shanmugam K. A comprehensive review on Ocimum basilicum Journal of Natural Remedies.

; 8(3):41–55. https://doi.org/10.18311/jnr/2018/21324

Sethuraman J, Nehru H, Shanmugam K, Balakrishnanan P. Evaluation of potent phytochemicals and antidiabetic activity of Ficusracemosa L. World Journal of Pharmaceutical Research. 2017; 6(15):909–20. https://doi:10.20959/wjpr201715-10140.

Balakrishnan P, Kumar GS, Ramalingam PS, Nagarasan S, Murugasan V, Shanmugam K. Distinctive pharmacological activities of Eclipta alba and it’s coumestanwedolactone. Indo American Journal of Pharmaceutical Research. 2018; 5(4):2996–3002. https://doi:10.5281/zenodo.1231062.

Saranya T, Kalimuthu J, Balakrishnan P, Ramalingam PS, Parthasarathi S, et al., Isolation and characterisation of cellulolytic activity of bacteria and fungi from the soil of paper recycling unit at periyarmaniammai university. Indo American Journal of Pharmaceutical Research. 2017; 7(06). https://doi: 10.5281/ZENODO.2526699.

Rathnasamy S, Rufus AL, Purusothaman. Comparative studies on isolation and characterization of allinase from garlic and onion using PEGylation- A novel method. Asian Journal of Chemistry. 2014; 26(12):3733–5. https://doi.org/10.14233/ajchem.2014.17064

Mathew R, Sankar DP. Effect of methyl jasmonate and chitosan on growth characteristics of Ocimum basilicum L., Ocimum sanctum L. and Ocimum gratissimum L. Cell suspension cultures. African Journal of Biotechnology. 2012; 11(21):4759–66. https://doi.org/10.5897/AJB11.3183

Sundaramoorthy M, Prabaharan C, Balakrishnan P, Saravanan TS. Antibacterial and wound healing effects of semi-purified heart proteins from certain selective slaughter house animals. Indo American Journal of Pharmaceutical Research. 2014; 4(01).

Grayer RJ, Bryan SE, Veitch NC, Goldstone FJ, Paton A, Wollenweber E. External flavones in sweet basil, Ocimum basilicum, and related taxa. Phytochemistry. 1996; 43(5):1041–47. https://doi.org/10.1016/S00319422(96)00430-X

Nagarasan S, Boominathan M. Perspective Pharmacological Activities of LeucasAspera: An Indigenous Plant Species. Indo American Journal of Pharmaceutical Research. 2016; 6(09):6567–72.

Hossain MA, Kabir MJ, Salehuddin SM, Mizanur Rahman SM, Das AK, Singha SK, Alam K, Rahman A. Antibacterial properties of essential oils and methanol extracts of sweet basil Ocimum basilicum occurring in Bangladesh. Pharmaceutical Biology. 2010; 48(5):504–11. https://doi.org/10.3109/13880200903190977. Mid:20645791

Filip S, Vidović S, Vladić S, Pavlić B, Adamović D, Zeković Z. Chemical composition and antioxidant properties of Ocimum basilicum L. extracts obtained by supercritical carbon dioxide extraction: Drug exhausting method. The Journal of Supercritical Fluids. 2016; 109:20–5. https://doi.org/10.1016/j.supflu.2015.11.006

Ramalingam PS, Sagayaraj M, Ravichandiran P, Balakrishnanan P, Nagarasan S, Shanmugam K. Lipid peroxidation and anti-obesity activity of Nigella sativa seeds. World Journal of Pharmaceutical Research. 2017; 6(10):882–92. https://doi.org/10.20959/wjpr201710-9389

Pirbalouti AG, Mahdad E, Craker L. Effects of drying methods on qualitative and quantitative properties of essential oil of two basil landraces. Food Chemistry. 2013; 141(3):2440–49. https://doi.org/10.1016/j.foodchem.2013.05.098. PMid:23870979

Balakrishnan P, Musafargani TA, Subrahmanyam S, Shanmugam K. A perspective on bioactive compounds from Solanum trilobatum. Journal of Chemical and Pharmaceutical Research. 2015; 7(8):507–12.

Soni V, Jha AK, Dwivedi J, Soni P. Qualitative and quantitative determination of phytoconstituents in some antifertility herbs. Indian Journal of Pharmaceutical Sciences. 2018; 80(1):79–84. https://doi.org/10.4172/pharmaceuticalsciences.1000332

Amrani S, Harnafi H, Gadi D, Mekhfi H, Legssyer A, Aziz M, et al. Vasorelaxant and anti-platelet aggregation effects of aqueous Ocimum basilicum extract. Journal of Ethanopharmacology. 2009; 125(1):157–62. https://doi.org/10.1016/j.jep.2009.05.043. PMid:19505553

Jayasinghe C, Gotoh N, Aoki T, Wada S. Phenolics composition and antioxidant activity of Sweet Basil (Ocimum basilicum L.). Journal of Agricultural and Food Chemistry. 2003; 51(15):4442–9. https://doi.org/10.1021/jf034269o. PMid:12848523

Marotti M, Piccaglia, R, Giovanelli E. Differences in essential oil composition of Basil (Ocimum basilicum L.) Italian cultivars related to morphological characteristics. Journal of Agricultural and Food Chemistry. 1996; 44(12):3926–29. https://doi.org/10.1021/jf9601067

Kadhim MJ, Sosa AA, Hameed IH. Evaluation of antibacterial activity and bioactive chemical analysis of Ocimum basilicum using Fourier Transform Infrared (FT-IR) and Gas Chromatography- Mass Spectrometry (GC-MS) techniques. International Journal of Pharmacognosy and Phytochemical Research. 2016; 8(6):127–46. https://doi.org/10.5897/JPP2015.0366

Chalchat J-C, Özcan MM. Comparative essential oil composition of flowers, leaves and stems of basil (Ocimum basilicum L.) used as herb. Food Chemistry. 2008; 110(2):501– 03 https://doi.org/10.1016/j.foodchem.2008.02.018. PMid:26049245


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