Unveiling the Unexplored and Critically Endangered Ilex khasiana for its Antioxidant Properties


  • Mizoram University, Department of Zoology, Tanhril, Mizoram, 796004, India
  • Regional Institute of Paramedical and Nursing Sciences, Department of Pharmacy, Zemabawk, Mizoram, 796017, India
  • Pachhunga University College, Department of Life Sciences, Aizawl, Mizoram, 796001, India


Ilex khasiana Purk. is an evergreen tree of Aquifoliaceae family reportedly endemic to Khasi Hills, Meghalaya, India; but it is also seen in a localised area of Aizawl, Mizoram, India. There is a vast history about the genus Ilex with reference to their medicinal uses in different traditional practices such as, in the treatment of coronary heart diseases, hypertension, hyperlipemia and hepatitis. Interestingly, I. khasiana is relatively unknown and yet it is known to possess multiple medicinal characteristics. The antioxidant potential studies were conducted on leaf extract specimens from Mizoram, using petroleum ether (IKP), chloroform (IKC) and methanol (IKM). IKM had the highest total antioxidant activity with 76.42 ascorbic acid equivalent (AE) mg/g, followed by IKP with 44.27 AE mg/g, and then by IKC with 17.08 AE mg/g respectively. The reducing power assay showed a concentration-dependent activity against potassium ferricyanide. The total phenolic content was found to be 3.46 gallic acid equivalent (GAE) mg/g for IKM and 1.450 GAE mg/g for IKC respectively. The total flavonoid content was 41.9 quercetin equivalent (QE) mg/g for IKC and 30.8 QE mg/g for IKM. IKP did not show any activity in phenolic and total flavonoid assays.


Antioxidant Activity, Flavonoids, Ilex khasiana, Phenolics, Reducing Power

Subject Collection

NE India

Subject Discipline

Life Sciences

Full Text:


Sharma GN, Gupta G, Sharma P. A comprehensive review of free radicals, antioxidants, and their relationship with human ailments. Critical Reviews in Eukaryotic Gene Expression. 2018; 28(2):139–54. https://doi.org/10.1615/ CritRevEukaryotGeneExpr.2018022258. PMid:30055541

Chen Q, Wang Q, Zhu J, Xiao Q, Zhang L. Reactive oxygen species: Key regulators in vascular health and diseases. British Journal of Pharmacology. 2018; 175(8):1279–92. https://doi.org/10.1111/bph.13828. PMid:28430357 PMCid:PMC58 67026

Parvez MK. Natural or plant products for the treatment of neurological disorders: Current knowledge. Current Drug Metabolism. 2018; 19(5):424–8. https://doi.org/10.2174/13 89200218666170710190249 PMid:28699506

Hao D, Gu X, Xiao P, Liang Z, Xu L, Peng Y. Research progress in the phytochemistry and biology of Ilex pharmaceutical resources. Acta Pharmaceutica Sinica B. 2013; 3(1):8–19. https://doi.org/10.1016/j.apsb.2012.12.008

The Plant List. Ilex [Internet]. [cited 2010]. Available from: http://www.theplantlist.org/browse/A/Aquifoliaceae/Ilex

CAMP Workshops on Medicinal Plants, India. Ilex khasiana. The IUCN Red List of Threatened Species 1998: e.T31239A9618655 [Internet]. [cited 2019 May 6]. Available from: http://dx.doi.org/10.2305/IUCN.UK.1998.RLTS.T31239A9618655.en.

Haridasan K, Rao RR. Forest Flora of Meghalaya, Vol. I. Dehradun, India: Bishen Singh Mahendra Pal Singh; 1985.

Adhikari D, Barik SK, Upadhaya K. Habitat distribution modelling for reintroduction of Ilex khasiana Purk., a critically endangered tree species of northeastern India. Ecological Engineering. 2012; 40:37–43. https://doi.org/10.1016/j.ecoleng.2011.12.004

Laloo RC, Kharlukhi L, Jeeva S, Mishra BP. Status of medicinal plants in the disturbed and the undisturbed sacred forests of Meghalaya, northeast India: Population structure and regeneration efficacy of some important species. Current Science. 2006; 25:225–32.

Sawmliana M. The Book of Mizoram Plants (2nd edition) Aizawl, India: Zakhuma; 2013. p. 142.

Hao D, Gu X, Xiao P, Liang Z, Xu L, Peng Y. Research progress in the phytochemistry and biology of Ilex pharmaceutical resources. Acta Pharmaceutica Sinica B. 2013; 3:8–19. https://doi.org/10.1016/j.apsb.2012.12.008

Tang W, Eisenbrand G. Ilex pubescens Hook et Arn. Chinese Drugs of Plant Origin. Berlin, Heidelberg: Springer; 1992. p. 593–6. https://doi.org/10.1007/978-3-642-73739-8_76

Koo A. Microvascular techniques for in vivo assay of vasoactive agents from Chinese medicinal herbs. Chang HM, Yeung HW, Tso WW, Koo A, editors. Advances in Chinese Medicinal Materials Research. Singapore: World Scientific; 1985. p. 559–80.

Zhang X, Ke M, Ou F. Extraction and isolation of total flavonoid glycosides of Mao Don Quing (Ilex pubescens) bark. Zhongcaoyao. 1981; 12:399.

Wenjuan Q, Xiue W, Junjie Z, Fukuyama Y, Yamada T, Nakagawa K. Triterpenoid glycosides from leaves of Ilex cornuta. Phytochemistry. 1986; 25:913–6. https://doi.org/10.1016/0031-9422(86)80026-7

Li L, Zhang Y, Zhang P, Pi H, Ruan H, Wu J. Appraisal of anti-inflammatory and free radical scavenging activities of ethanol extract of Ilex ficoidea Hemsl and Ilex centrochinensis SY Hu. Environmental Toxicology and Pharmacology. 2011; 32:122–7. https://doi.org/10.1016/j.etap.2011.04.003. PMid:21843790

Bracesco N, Sanchez AG, Contreras V, Menini T, Gugliucci A. Recent advances on Ilex paraguariensis research: minireview. Journal of Ethnopharmacology. 2011; 136:378–84.

https://doi.org/10.1016/j.jep.2010.06.032. PMid:20599603

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical Biochemistry. 1999; 269:337–41. https://doi.org/10.1006/abio.1999.4019. PMid:10222007

Oyaizu M. Studies on products of browning reaction. The Japanese Journal of Nutrition and Dietetics. 1986; 44(6):307– 15. https://doi.org/10.5264/eiyogakuzashi.44.307

Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture. 1965; 16:144–58.

Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry. 1999; 64:555–9. https://doi.org/10.1016/S0308-8146(98)00102-2

Dang JC, Kumaria S, Kumar S, Tandon P. Micropropagation of Ilex khasiana, a critically endangered and endemic holly of Northeast India. AoB Plants. 2011; 2011:plr012. https://doi.org/10.1093/aobpla/plr012. PMid:22476482 PMCid:PMC 3129536

Sardesai VM. Role of antioxidants in health maintenance. Nutrition in Clinical Practice. 1995; 10:19–25. https://doi.org/10.1177/011542659501000119. PMid:7898413

Warner HR. Superoxide dismutase, aging, and degenerative disease. Free Radical Biology and Medicine. 1994; 17:249– 58. https://doi.org/10.1016/0891-5849(94)90080-9

Gülçin İ. Antioxidant activity of food constituents: An overview. Archives of Toxicology. 2012; 86:345–91. https:// doi.org/10.1007/s00204-011-0774-2. PMid:22102161

Prior RL, Cao G. In vivo total antioxidant capacity: Comparison of different analytical methods1. Free Radical Biology and Medicine. 1999; 27:1173–81. https://doi.org/10.1016/S0891-5849(99)00203-8

Köksal E, Gülçin İ. Antioxidant activity of cauliflower (Brassica oleracea L.). Turkish Journal of Agriculture and Forestry. 2008; 32:65–78.

Pereira D, Valentão P, Pereira J, Andrade P. Phenolics: From chemistry to biology. Molecules. 2009; 14:2202–11. https:// doi.org/10.3390/molecules14062202. PMCid:PMC6254163

Valentão P, Fernandes E, Carvalho F, Andrade PB, Seabra RM, Bastos ML. Hydroxyl radical and hypochlorous acid scavenging activity of small centaury (Centaurium erythraea) infusion. A comparative study with green tea (Camellia sinensis). Phytomedicine. 2003; 10:517–22. https://doi.org/10.1078/094471103322331485. PMid:13678237

Choi HR, Choi JS, Han YN, Bae SJ, Chung HY. Peroxynitrite scavenging activity of herb extracts. Phytotherapy Research. 2002; 16:364–7. https://doi.org/10.1002/ptr.904. PMid:12112294

Parr AJ, Bolwell GP. Phenols in the plant and in man. The potential for possible nutritional enhancement of the diet by modifying the phenols content or profile. Journal of the Science of Food and Agriculture. 2000; 80:985–1012. https:// doi.org/10.1002/(SICI)1097-0010(20000515)80:7<985::AIDJSFA572> 3.0.CO;2-7

Cos P, Ying L, Calomme M, Hu JP, Cimanga K, Van Poel B, Pieters L, Vlietinck AJ, Berghe DV. Structure − activity relationship and classification of flavonoids as inhibitors of xanthine oxidase and superoxide scavengers. Journal of Natural Products. 1998; 61:71–6. https://doi.org/10.1021/ np970237h. PMid:9461655

Bruneton J. Huiles essentielles. Pharmacognosie, phytochimie, plantes médicinales (3rd edition). Paris, France: Lavoisier; 1999.

Cushnie TT, Lamb AJ. Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents. 2005; 26:343–56. https://doi.org/10.1016/j.ijantimicag.2005.09.002. PMid:16323269


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