The Role of Histaminergic H2 Receptors on Spasmolytic Activity of Hydroalcoholic Extract of Parsley (Petroselinum crispum) Seeds in Isolated Rat’s Ileum


Affiliations

  • Department of Biology, Shahid Chamran University, Ahwaz, Iran, Islamic Republic of
  • Department of Food Hygiene, Faculty of Veterinary Medicine,Shahid Chamran, Ahwaz, Iran, Islamic Republic of

Abstract

Objective: The long traditional history of anti-spasmodic effects of Petroselinum Crispum on the gastrointestinal system, and considering histamine receptors in rats ileum, to elucidate the role of the histamine receptor in function relate intestinal motility in this study investigate the effects of histamine and antagonists H2 receptor (Cimetidine) on contractions of adult male rat’s ileum. Methods: In this study, 2 cm of terminal isolated adult male rat’s ileum in an organ bath containing the oxygenated Tyrode solution (370c, PH = 7.4) was used and placed under 1 gram tension contraction induced by 60mM potassium chloride. The effect of parsley extract was measured in presence of histamine and antagonists H2 receptor (cimetidine) and their effects on the rat،s ileum contractions were recorded by using Harvard oscillograph. Results: The parsley extract reduced ileum contraction induced by KCl in a dose-dependent manner (P<0.001, n = 7). Spasmolytic effect of extract after tissue exposure to histamine (0.1 mg/ml) as an agonist and cimetidine (0.004 mg/ml) did not decrease ileum contractions. Conclusion: Although Parsley seeds extract reduced rat ileum contraction, it seems that H2 histamine receptors (cimetidine) are not involved in this activity.

Keywords

Cimetidin, Contraction, Histamine, Isolated Ileum, Rat’s

Full Text:

References

Mahmood S, Hussain Sh, Malik F. Critique of medicinal of conspicuousness Parsley (Petroselinum crispum): A culinary herb of Mediterranean region. P J Pharmacol. 2014; 27(1):193-202.

Naseri MKG, Moghaddam MA, Bahadur S.Antispasmodic effect of Tecoma stans (L.) Juss leaf extract on rat ileum. DARU. 2007; 15:3.

Bigovic D, Brankovic S, Kitic D, Radenkovic M, Jankovic T, Savikin K, Zivanovic S. Relaxant effect of the ethanol extract of Helichrysum plicatum Asteraceae on isolated Rat Ileum contractions. J of Molec. 2010; 15:3391–401. Crossref. PMid:20657488

Mamadou G, Meddah B, Limas-Nzouzi N, El Haj AA, Bipolo S, Mokondjimobé E, Mahraoui L, Faouzi MA, Ducroc R, Cherrah Y, Eto B. Antispasmodic phytomedicine from traditional, utilization to rational formulation: The functional approach.Phytopharmacol. 2011; 1(3):20–3.

Moradi MT, Rafieian-Koupaei M, Imani-Rastabi R, Nasiri J, Shahrani M, Rabiei Z, Alibabaei Z. Antispasmodic effects of yarrow (Achillea millefolium l.) extract in the isolated ileum of the rat.J of Pharmacol. 2013; 10(6):499–503. Crossref.

ELai-Har Tang E, Rajarajeswaran, Fung Sh Y, Kanthimathi MS. Petroselinum crispum has antioxidant properties, protects against DNA damage and inhibits proliferation and migration of cancer cells. J of the Science of Food and Agriculture.2015; 95:2763–71. Crossref. PMid:25582089 PMCid:PMC5024025

Antal I, Edit Pápay Z. Study on the antioxidant activity during the formulation of the biological active ingredient. Euroscientific J. 2014; 3:1857–743.

Moazedi AA, Mirzaie DN, Seyyednejad SM, Zadkarami MR, Amirzargar A. Spasmolytic effect of Petroselinum crispum (parsley) on Rat s Ileum at different calcium chlorid concentration. Paki J of Bilogical Sciences. 2007; 10(22):4036–42. Crossref.

Hansen MB. Neurohumoral control of gastrointestinal motility. Physio Res. 2003; 52:1–30. PMid:12625803 10. Grasa L, Rebollar E, Arruebo MP, Plaza MA, Murillo MD. The role of Ca2+ in the contractility of rabbit small intestine in vitro. J of Physio and Pharmacol. 2004; 55(3):639–50. PMid:15381833

Sedighi M, Kopaei MR, Ahmadabadi MN, Godarzi I, Baradaran A. In vitro impact of hydro alcoholic extract of rosa damascena mill on Rat Ileum contractions and the mechanisms involved. Int J of Preventive Medi. 2014; 5(6):775–67.

Soto SE, Maldonado DG, Espana PC, Grespo FA, Salcedo SJC. Spasmoiytic effect of Mentha pulegium L. Involves ionic flux regulation in rat ileum scripts.Smooth Muscle Research. 2010; 46(2):107–17.Crossref.

Desch I, Leurs R. Histamine receptors. Tocris Bios.2008; 29:2.

Smolinski S, Jutel M, Crameri R, Mahony LO. Histamine and gut mucosal immune regulation. Eur J of Allergy and Clinical Immunol. 2014; 69:273–81.Crossref.PMid:24286351

Hallgren J, Gurish MF. Granule maturation in mast cells: Histamine in control. Eur J of Immunol. 2014; 44:33–36. Crossref. PMid:24319003

Shahid M, Tripathi T, Sobia F, Moin Sh, Siddiqui M, Ali Khan R. Histamine, histamine receptors, and their role in immunomodulation: An updated systematic review. The Open Immunol J. 2009; 2:9– 41. Crossref.

Yamanaka K. Kitamura K. Electrophysiological and mechanical characteristics of histamine receptors in smooth muscles cells of the guniea pig ileum. Eur J of Pharmacol. 1987; 144:9–37.

Koutsovlti-Papadopoulos M, Kounenis G, Elezoglou V, Kokolis N. Effect of cimetidine on the histamine and dimaprit induced responses on the guinea pig ileum during development. Gen Pharmacol. 1993; 24(4):1021–6.Crossref.

Damabi NM, Moazedi AA, Seyyednejad SM. The role of α- and -β adrenergic receptors in the spasmolytic effects on rat ileum of Petroselinum crispum Latifolum (parsley). Asian Pac Jof Tro Medicine. 2010; 866–70.

Nasu T, Murase h, Shibata H. Manganese Ions induce tonic contraction after relaxation in a high-K+ medium in the ileal longitudinal smooth muscle of guinea-pig. J Pharm Pharmacol. 1994 Sep; 46(9):735– 9. Crossref.PMid:7837042

Dale MM. An inhibitory effect of acetylcholine on the response of the guinea-pig ileum to histamine.British J of Pharmacol. 1958; 13(1):17. Crossref.

Vyas S, Verma SCb. The direct excitatory effects of cimetidine on the smooth muscle of guinea pig ileum. Agents and Actions. 1981; 11:3. Crossref.

Jagadeesh K, Revankar Sh, Jagadeesh SC. Spasmolytic effect of ethanolic extract of calotropis procera leaves on in-vitro guinea pig ileum. Asian Pac J Health Sci.2014; 1(2):65–8.

Bolton TB. Mechanisms of action of transmitters and other substances on smooth muscle. Am Physio Society. 1979; 59(3):607–718. Crossref.

Morel N, Paul Hardy J, Godfraind T. Histamineoperated calcium channels in the intestinal smooth muscle of the guinea-pig. Eur J of Pharmacol. 1987; 13(5):69–75. Crossref.

Van Crombruggen K, Van Nassauw L, Timmermans JP, Lefebvre RA. Inhibitory purinergic P2 receptor characterization in rat distal colon. Neuropharmacol J. 2007; 53:57–71. Crossref. PMid:17612577

Daude RB, Mishra SV, Chega de RY, Amrutkar SV.Bioassay of histamine by using isolated chicken ileum. World J of Pharmacy and Pharmaceutical Science. 2016; 5(1):1221–25.

Singh SH, Mandal MB. In vitro study of acetylcholine and histamine-induced contractions in colon and rectum of adult and neonate rats. Indian J of Physiolo Pharmacol. 2013; 57(2).

Mascolo N, Izzo AA, Costa M, Capasso F. The role of histamine H1, H 2 and H3 receptors on enteric ascending synaptic transmission in the guinea pig Ileum. The Am Society for Pharmacol and Experi Therapeutics. 1998; 287:952–7.

Deiteren A, De Man JG, Pelckmans PA, De Winter BY. Histamine H4 receptors in the gastrointestinal tract. British J of Pharmacol. 2015; 172:1165–78.Crossref.PMid:25363289 PMCid:PMC4337694


Refbacks

  • There are currently no refbacks.