Isolation of Mesophyll Protoplast from Indian Mulberry (Morus alba L) CV. S1635

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Authors

  • Pijush Mallick
     Division of Plant Biology, Bose Institute, P-1/12, C.I.T., Scheme VII M, Kolkata-700 054, West Bengal ,IN
  • Sayantan Ghosh
     Division of Plant Biology, Bose Institute, P-1/12, C.I.T., Scheme VII M, Kolkata-700 054, West Bengal ,IN
  • Shruti Chattaraj
     Division of Plant Biology, Bose Institute, P-1/12, C.I.T., Scheme VII M, Kolkata-700 054, West Bengal ,IN
  • Samir Ranjan Sikdar
     Division of Plant Biology, Bose Institute, P-1/12, C.I.T., Scheme VII M, Kolkata-700 054, West Bengal ,IN

Keywords:

Mulberry Plant, Mesophyll Protoplast, Cell Wall Degradating Enzymes.

Abstract

Mulberry (Morus alba L. cv S1635) is a perennial crop plant in Indian sericulture and used for silkworm Bombyx mori L. feeding. Qualitative and quantitative improvement of this crop is highly essential in respect to better and quality silk production and hence through breeding approaches the development of new lines is important. Protoplasts are the good source of any plant to study about their genetics and cellular mechanisms for breeding. Using cell wall degradating enzymes mulberry protoplasts from mesophyll tissue were isolated. Several combinations of cellulose, macerozyme and driselase enzymes mixture were tested in different incubation conditions. A combination of Cellulase R10-1%, Macerozyme R10-1% and Driselase-0.25% in 0.6M mannitol showed comparatively better yield (3.8 í— 106 / gm tissue) after 3-4 hrs shaking with 100 rpm at 28°C.

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Published

2016-12-01

Issue

Volume 13, Issue 2, December 2016

Section

Articles

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