Analysis of Genetic Diversity of Protoplast Fused Mushrooms Pleurotus Florida X Lentinus Squorrosulus (PFLS) Somatic Hybrids through Rapd Markers

Abstract

A total of twelve somatic hybrid strains, Pleurotus florida x Lentinus squorrosulus (pfls), were characterized through PCR based RAPD analysis. Sixteen random decemer primers yielded a total of 136 fragments with 97.79 % polymorphism with an amplicon range of 200 bp - 3000 bp in size. RAPD-08 showed optimum amplification and generated 12 scorable bands (250 bp -1800 bp) where RAPD-07 amplified least and generated 5 scorable bands (400 bp - 1300 bp). PIC ranged from 0.424 (SRS-15) to 0.499 (SRS-16). Based on Jaccard’s proximity matrix the highest genetic distance was found in between parent L. squarrosulus and hybrid pfls1q which means they are genetically most distantly related. Among the hybrid pfls1k and pfls1p showed maximum genetic distance while the most closely related hybrids were pfls1i and pfls1l. The parent L. squarrosulus was most genetically distant individual than the other parent P. florida.