Volume 20, Issue 1, June 2016
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The daily expression profile of Neuropeptides (gnih, gnrh3, kiss1 and kiss2): A study of possible interaction in the brain of Zebrafish (Danio rerio)


  • Biological Rhythm Laboratory, Animal Resources Programme, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Takyelpat,Manipur,Imphal, 795001, India
  • Distributed Information Sub-Centre, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Manipur,Imphal, 795001, India
  • Biological Rhythm Laboratory, Animal Resources Programme, Institute of Bioresources and Sustainable Development, Development, Department of Biotechnology, Government of India, Manipur,Imphal, 795001, India
  • Biological Rhythm Laboratory, Animal Resources Programme, Institute of Bioresources and Sustainable Development, Department of Biotechnology,Government of India, Manipur,Imphal, 795001, India
  • Biological Rhythm Laboratory, Animal Resources Programme, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Manipur,Imphal, 795001, India
  • Department of Biotechnology, Gauhati University, Guwahati, Assam, 781014, India


Involvement of neuropeptides in the reproduction of fish (seasonal/regular) is known. The daily rhythmicity and their possible interaction of four major neuropeptides namely gnih, gonadotropin-inhibitory hormone; gnrh, gonadotropinreleasing hormone; kiss1/2, kisspeptin 1/2; is not known to any fish. Our present study on the whole brain of zebrafish (Danio rerio) aimed at the daily rhythmicity of the mRNA expression of these four neuropeptides in a 12 h light/12 h dark photoperiod (LD). Only kiss2 in its expression gives a rhythmicity but other three peptides are not rhythmic. Moreover, the expression of gnih is 10-fold lower than gnrh3. Our STRING network analysis suggests kiss2 act as the mediator to communicate with gnih, gnrh3, and kiss1. Our present finding is indicating the important role of kiss2 in mediating the reproductive signal and may play a central role in the synchronization of the environmental signal and reproductive periodicity.


gnih, gnrh3, kiss1, kiss2, Protein-Protein Interaction, Zebrafish

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