Isolates of the entomopathogenic mitosporic ascomycete, Beauveria bassiana were evaluated against Spodoptera litura using leaf spray method. Mortality of 66.67, 73.33 and 80.0% with respect to the isolates Bb02, Bb09 and Bb10, respectively, was obtained four days after treatment. LC50 of the isolates was 2.1í—106, 3.6í—107 and 1.2í—107 conidia ml-1 for Bb02, Bb09and Bb10, respectively. The LT50 value for Bb02 and Bb09 was 4.8 days, whereas it was 4.0 days for Bb10 @108 spore mL-1. The germination percentage was 79.83, 88.33 and 95.53% for Bb02, Bb09 and Bb10, respectively Bb10 isolate was the most virulent with potential for the management of S. litura.