Potential Use of Elicitors from Trichoderma in Induced Systemic Resistance for the Management of Phytophthora capsici in Red Pepper


  • National Bureau of Agriculturally Important Insects, Bangalore, Karnataka, 560024, India
  • Bharathidasan University, Department of Plant Science, School of Life Sciences, Tiruchirapalli, Tamil Nadu, 620024, India


Eleven isolates of Trichoderma harzianum were screened for their potential to induce systemic resistance against Phytophthora capsici in red pepper plants. The effect of talc formulations of these eleven isolates on induction of glucanase activity and phenol content was studied. There was a significant increase in glucanase activity in plants treated with Th8 and Th1 (94 and 90 μg glucose released min-1 g-1) compared to control (77 μg). Similarly phenol content also increased in plants treated with Th4, Th7 and Th10 (48-59 μg g-1) compared to control (34 μg g-1). Cell wall glucan elicitors were extracted from T. harzianum isolate Th10 that had been reported earlier as an efficient biocontrol agent. Treatment with elicitor preparations also induced high glucanase activity (40 μg glucose released min-1 g-1) and increased phenol content (42 μg g-1) compared to control where the glucanase activity was 33 μg glucose released min-1 g-1 while phenol content was 27 μg g-1. Similarly the elicitor treatment as seedling dip reduced P. capsici infection to 23% compared to control (93%). The potential use of ISR eliciting isolates in the biocontrol of P. capsici in red pepper is discussed.


Cell Wall Glucan Elicitor, Induced Systemic Resistance (ISR), Phytophthora capsici, Red Pepper, Trichoderma harzianum.

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Ahmed, A. S., Sanchez, C. P. and Candela, M. E. 2000.Evaluation of induction of systemic resistance inpepper plants (Capsicum annuum) to Phytophthoracapsici using Trichoderma harzianum and itsrelation with capsidiol accumulation. EuropeanJournal of Plant Pathology, 106: 817–824.

Bradford, M. M. 1976. A rapid and sensitive method for thequantification of microgram quantities of proteinutilizing the principle of protein-dye binding.Analytical Biochemistry, 72: 248–254.

Elad, Y. 2000. Biological control of foliar pathogens bymeans of Trichoderma harzianum and potentialmodes of action. Crop Protection, 19: 709–714.

Elad, Y, Chet, I. and Henis, Y. 1981. A selective medium forimproving quantitative isolation of Trichodermaspp. from soil. Phytoparasitica, 9: 59–67.

Elad, Y., Kirshner, B., Yehuda, N. and Sztejnberg, A. 1998.Management of powdery mildew and gray mouldof cucumber by Trichoderma harzianum T39and Ampelomyces quisqualis AQ10. Biocontrol,43: 241–251.

Folin, O. and Ciocalteu, V. 1927. On tyrosine and tryptophandeterminations in proteins. The Journal ofBiological Chemistry, 78: 627–650.

Harmann, G. E., Howell, C. R., Viterbo, A., Chet, I. andLorto, M. 2004. Trichoderma species–opportunistic, avirulent and plant symbionts. Nature Review of Microbiology, 2: 43–56.

Hanania, U. and Avni , A. 1997. High-affinity bindingsite for ethylene-inducing xylanase elicitor onNicotiana tabacum membranes. Plant Journal,12: 113–120.

Hoitink, H. A. J., Madden, L. V. and Dorrance, A. E. 2006. Systemic resistance induced by Trichoderma spp.:Interactions between the host, the pathogen, thebiocontrol agent, and soil organic matter quality. Phytopathology, 96: 186–189.

Khan, J., Ooka, J. J., Miller, S. A., Madden, L. V. andHoitink, H. A. J. 2004. Systemic resistance inducedby Trichoderma hamatum 382 in cucumber againstPhytophthora crown rot and leaf blight. PlantDisease, 88: 280–286.

Pan, S. Q., Ye, X. S., Kuc, J. 1991. A technique fordetection of chitinase, beta-1,3-glucanase, andprotein patterns after a single separation usingpoly-acrylamide gel electrophoresis or isoelectrofocusing. Phytopathology, 81: 970–974.

Prasad, R. D., Rangeshwaran, R., Hegde, S. V. and Anuroop,C. A. 2002. Effect of soil and seed application ofTrichoderma harzianum on pigeonpea wilt causedby Fusarium udum under field conditions. CropProtection, 21: 293–297.

Ramakrishnan, G., Jeyarajan, R. and Dinakaran, D. 1994. Talc based formulation of Trichoderma viride for biocontrol of Macrophomina phaseolina. Journalof Biological Control, 8: 41–44.

Rudresh, D. L., Shivaprakash, M. K. and Prasad, R. D. 2005. Tricalcium phosphate solubilizing abilitiesof Trichoderma sp. in relation to P uptake andgrowth and yield parameters of chickpea (Cicerarietinum L.). Canadian Journal of Microbiology,51: 217–222.

Sharp, J. K., McNeil, M. and Albersheim, P. 1984. Theprimary structure of one elicitor active and sevenelicitor inactive hexa-beta-D-gluco-pyranosyl-D-glucitols isolated from the mycelial walls ofPhytophthora megasperma f. sp. glycinea. Journalof Biological Chemistry, 259: 11321–11336.

Sriram, S., Misra, R. S., Sahu, A. K. and Maheswari,S. K. 2003. A cell wall glucan elicitor inducesresistance in taro against Phytophthora leaf blight. Journal of Plant Disease and Protection, 11: 17–26.

Singh, D. 1991. Biocontrol of Sclerotinia sclerotiorum (Lib.)de Bary by Trichoderma harzianum. Tropical PestManagement, 37: 374–378.

Woo, S. L., Scala, F., Ruocco, M. and Lorito, M. 2006. The molecular biology of the interactions betweenTrichoderma spp., phytopathogenic fungi, andplants. Phytopathology, 96: 181–185.

Yedidia, I., Benhamou, N. and Chet, I. 1999. Induction ofdefence in cucumber plants (Cucumis sativus L.)by the biocontrol agent Trichoderma harzianum. Applied Environmental Microbiology, 65: 1061–1070.

Yedidia, I., Benhamou, N., Kapulnik, Y. and Chet, I. 2000. Induction and accumulation of PR proteinsactivity during early stages of root colonizationby the mycoparasite Trichoderma harzianumstrain T-203. Plant Physiology and Biochemistry,38: 863–873.

Zong, J. and BingSheng, L. 1995. The influence of thepreparation of Trichoderma koningii on somephysiological and biochemical properties ofcotton and kidney bean seedlings. ChineseJournal of Biological Control, 11: 30–32.


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