Molecular Diversity and Antibiotic Sensitivity of Gut Bacterial Symbionts of Fruit Fly, Bactrocera tau Walker

Jump To References Section

Authors

  • Department of Entomology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur 176062, Himachal Pradesh ,IN
  • Department of Entomology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur 176062, Himachal Pradesh ,IN

DOI:

https://doi.org/10.18311/jbc/2009/3667

Keywords:

Bacterial Symbionts, Bactrocera tau, recA gene, rrs gene, RAPD and REP–PCR Analysis.

Abstract

Random amplified polymorphic DNA-Repetitive extragenic palindromic (RAPD- REP) PCR-restriction length polymorphism (PCR-RFLP) were used to study the genetic similarity among bacterial symbionts of fruit fly, Bactrocera tau Walker. RAPD-PCR and REP-PCR placed three bacteria into two groups, viz., group A (BC1 and BC3) and group B (BC2). However, PCR- RFLP analysis of recA and rrs genes of bacterial symbiont placed BC1 and BC2 into group A and BC3 into group B. Studies on antibiotic sensitivity pattern also supported the results of PCR-RFLP as the response of BC1 and BC2 was similar to different antibiotics. All the bacterial symboints were found to be sensitive to co-trimoxazole, gentamycene and nofloxacin. The findings suggest that there may be a possibility of utilising these antibiotics for disruption of fruit fly biology as foliar symbioticides for their management.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Downloads

Published

2009-10-17

How to Cite

Sood, P., & Prabhakar, C. S. (2009). Molecular Diversity and Antibiotic Sensitivity of Gut Bacterial Symbionts of Fruit Fly, <I>Bactrocera tau</I> Walker. Journal of Biological Control, 23(3), 213–220. https://doi.org/10.18311/jbc/2009/3667

 

References

Amann, R. I., Ludwig, W. and Schleifer, K. H. 1995.Phylogenetic identification and in situ detectionof individual microbial cells without cultivation.Microbiological Review, 59: 143–169.

Bauer, S., Tholen, A., Overmann, J. and Brune, A. 2000.Characterization of abundance and diversity oflactic acid bacteria in the hindgut of wood andsoil-feeding termites by molecular and culture–dependent techniques. Archives of Microbiology,173: 126–137.

Behar, A., Yuval, B. and Jurkevitch, E. 2005. Enterobacteria–mediated nitrogen fixation in natural populationsof the fruit fly, Ceratitis capitata. MolecularEcology, 14: 2637–2643.

Belcari, A. and Bobbio, E. 1999. The use of copper in controlof the olive fly – Bactrocera oleae. InformatoreFitopatologico, 49: 52–55.

Blackwood, K. S., He, C., Gunton, J., Turenne, C. Y., Wolfe,J. and Kabani, A. M. 2000. Evaluation of recAsequences for identification of Mycobacteriumspecies. Journal of Clinical Microbiology, 38:2846–2852.

de Vries, E. J., Breeuwer, J. A. J. and Jacobs, G. 2001a.The association of flower thrips, Frankliniellaoccidentalis, with a near Erwinia species gutbacteria: transient or permanent? Journal ofInvertebrate Pathology, 77: 120-128.

de Vries, E. J., Jacobs, G. and Breeuwer, J. A. J. 2001b.Growth and transmission of gut bacteriain the western flower thrips, Frankliniellaoccidentalis. Journal of Invertebrate Pathology,77: 129–137.

Dillon, R. J. and Dillon, V. M. 2004. The gut bacteria ofinsects: Nonpathogenic Interactions. AnnualReview of Entomology, 49: 71–92.

Eisen, J. A. 1995. The recA protein as a model moleculefor molecular systematic studies of bacteria:comparison of trees of recAs and 16S RNAsfrom the same species. Journal of MolecularEvolution, 41: 1105–1123.

Gupta, K. and Anand, M. 2003. In vitro vitamin synthesis bythe symbiotes of Bactrocera cucurbitae. IndianJournal of Entomology, 65: 518–528.

Head, I. M., Saunders, J. R. and Piclup, R. W. 1998.Microbiological evolution, diversity andecology: A decade of ribosomal RNA analysisof uncultivated microorganisms. MicrobialEcology, 35: 1–21.

Heddi, A., Charles, H., Khatchadourian, C., Bonnot, G. andNardon, P. 1998. Molecular characterization ofthe principal symbiotic bacteria of the weevilSitophilus oryzae: A peculiar G + C content ofan endocytobiotic DNA. Journal of MolecularEvolution, 47: 52–61.

Hughes, V. M. and Datta, N. 1983. Conjugative plasmidin bacteria of the "pre–antibiotic era”. Nature,302: 725–726.

Huque, R. 2006. Comparative studies on the susceptibilityof various vegetables to Bactrocera tau (Diptera:Tephritidae). Pakistan Journal of BiologicalSciences, 9: 93–95.

Jang, E. B. and Nishijima, K. A. 1990. Identification andattractancy of bacteria associated with Dacusdorsalis (Diptera: Tephritidae). EnvironmentalEntomology, 19: 1726–1731.

Karlin, S., Weinstock, G. M. and Brendel, V. 1999. Bacterialclassification derived from recA proteinsequence comparisons. Journal of Bacteriology,177: 6881–6893.

Lauzon, C. R., Sjogren, R. E. and Prokopy, R. J. 2000.Enzymatic capabilities of bacteria associatedwith apple maggot flies, a postulated rolein attraction. Journal of Chemical Ecology,26: 953–967.

Lauzon, C. R., Sjogren, R. E., Wright, S. E. and Prokopy,R. J. 1998. Attraction of Rhagoletis pomonella(Diptera: Tephritidae) flies to odour of bacteria:apparent confinement of specialized members ofEnterobacteriaceae. Environmental Entomology,27: 853–857.

Lloyd, A. C., Drew, R. A. I., Teakle, D. S. and Hayward,A. C. 1986. Bacteria associated with someDacus species (Diptera: Tephritidae) and theirhost fruits in Queensland. Australian Journal ofBiological Sciences, 39: 361–368.

Lloyd, A. T. and Sharp, P. M. 1993. Evolution of the recAgene and molecular phylogeny of bacteria.Journal of Molecular Evolution, 37: 399–407.

Mahenthiralingam, E., Bischof, J., Byrne, S. K., Radomski,C., Davies, J. E., Av–Gay, Y. and Vandamme,P. 2000. DNA based diagnostic approaches foridentification of Bulkholderia cepacia complex,Bulkholderia vietnamiensis, Bulkholderiamultivorans, Bulkholderia stabilis andBulkholderia cepacia genomovars I and III.Journal of Clinical Microbiology, 130: 1893–3173.

Moran, N. A., McCutcheon, J. P. and Nakabachi, A. 2008.Genomics and evolution of heritable bacterialsymbionts. Annual Review of Genetics, 42:165–190.

Murphy, K. M., Teakle, D. S. and MacRae, I. C. 1994.Kinetics of colonization of adult Queenslandfruit flies (Bactrocera tryoni) by dinitrogen–fixing alimentary tract bacteria. Applied andEnvironmental Microbiology, 60: 2508–2517.

Nowak, A. and Kur, J. 1995. Genomic species typing ofacinetobacters by polymerase chain reactionamplification of the recA gene. FEMSMicrobiology Letters, 130: 327–332.

Oliver, K. M., Moran, N. A., Hunter, M. S. 2005. Variationin resistance to parasitism in aphids is due tosymbionts and not host genotype. Proceedingsof National Academy of Science, USA, 102:12795–12800.

Rohlf, F. J. 1993. NTSYS–pc: Numerical taxonomy andmultivariate analysis system version 1.80. Extersoftware. Setauket, New York.

Sardana, H. R., Tyagi, A. and Singh, A. 2005. KnowledgeResources on Fruit flies (Tephritidae: Diptera)in India. National Centre for Integrated PestManagement, New Delhi.

Sharma, P. N., Kaur, M., Sharma, O. P., Sharma, P. andPathania, A. 2005. Morphological, pathologicaland molecular variability in Colletotrichumcapsici, the cause of fruit rot of chillies in thesubtropical region of North–Western India. Journal of Phytopathology, 153: 141–148.

Sood, P. and Nath, A. 1999. Fruit flies associated withcucurbits in Himachal Pradesh. Journal of HillResearch, 12: 52–54.

Stouthamer, R., Breeuwer, J. A. J. and Hurst, G. D. D. 1999.Wolbachia pipientis: microbial manipulatorof arthropod reproduction. Annual Review ofMicrobiology, 53: 71–102.

Thakur, D., Bhuyan, M., Majumder, S., Yadav, A., Hazarika,L. K., Barman, N. K., Baruah, A. A. L. H. andBora, T. C. 2005. Isolation, characterization,in vitro antibiotic susceptibility and pesticidetolerance of gut bacteria from rice hispa,Dicladispa armigera (Olivier). Indian Journalof Microbiology, 45: 217–221.

Thompson, F. E. 1998. Fruit fly expert identification systemand systematic information data base. Myia,9: 594. http://www.sel.barc.usda.gov/Diptera/Myia/myia.htm.

Waleron, M., Waleron, K., Podhajska, A. J. and Lojkowska,E. 2002. Genotyping of bacteria belongingto the former Erwinia genus by PCR–RFLPanalysis of a recA gene fragment. Microbiology,148: 583–595.

Werren, J. H. and O'Neill, S. L. 1997. The evolution ofheritable symbionts, pp. 1–41. In: O'Neill, S.L., Hoffman, A. A. and Werren, J. H. (Eds.),Influential Passengers. Oxford University Press,Oxford.

White, I. M. and Elson-Harris, M. M. 1992. Fruit flies ofeconomic significance: Their Identificationand Bionomics. Centre for Agriculture andBiosciences International, Wallingford, U.K.,601p.