Influence of Laboratory Culturing of Paecilomyces lilacinus (Thomson) Sams, and Pochonia chlamydosporiazare et Al. on Spore Viability and Infeetivity against Meloidogyne incognita Chitwood Eggs
Two native isolates each of Paecilomyces lilacinus (Thomson) Sams. (PDBC PL55 and PDBC PLS8) and Pochonia chlamydosporia Zare et al. (PDBC VC56 and PDBC VC57) were subjected to repeated sub-culturing for ten generations at 28 ± 1°C with intervening resting periods in refrigerated conditions to evaluate the effect on their spore viability and infeetivity. Significant decrease in spore viability was noticed from generation IX in case of P. lilacinus isolates (PDBC PLSS and 58), while, P. chlamydosporia (PDBC VCS6 and 57) isolates recorded significant reduction in spore viability from generation VII. The infeetivity of the P. lilacinus and P. chlamydosporia isolates under study were on par with each other in ail the generations, which recorded significant reduction in tenth generation. Between the spore viability and infectivity of the isolates under report, spore viability was more influenced by repeated sub-culturing than infeetivity.
Infectivity, Meloidogyne incognita, Paecilomyces lilacinus, Pochonia chlamydosporia, Spore Viability, Sub-Culturing.
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