In silico Docking Studies on Cytochrome P450 Enzymes of Helicoverpa armigera (Hubner) and Trichogramma cacoeciae Marchal and Implications for Insecticide Detoxification

Jump To References Section

Authors

  • Molecular Entomology Laboratory, National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka ,IN
  • Molecular Entomology Laboratory, National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka ,IN
  • Molecular Entomology Laboratory, National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka ,IN
  • Molecular Entomology Laboratory, National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka ,IN
  • Molecular Entomology Laboratory, National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka ,IN
  • Molecular Entomology Laboratory, National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka ,IN
  • Molecular Entomology Laboratory, National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka ,IN

DOI:

https://doi.org/10.18311/jbc/2013/3450

Keywords:

Cytochrome P450, Helicoverpa armigera, Trichogramma cacoeciae, In silico Molecular Docking.

Abstract

In silico docking of cytochrome P450 monooxygenase (CYP450) of an insect, Helicoverpa armigera (Hübner) and a parasitoid, Trichogramma cacoeciae Marchal was studied with two insecticides, monocrotophos and fenvalerate. The CYP450 sequences of H. armigera (CYP9A12), T. cacoeciae (CYP4G12) and a human microsomal sequence CYP3A4, as positive control were retrieved from NCBI's GenBank database. The structure, as predicted by SOPMA, of CYP450 in H. armigera contained 78.7% helix and 43.3% sheets, while that of T. cacoeciae contained 60.6% helix and 68.5% sheets. The three-dimensional molecular models of CYP450 of H. armigera and T. cacoeciae indicated that 96.5 and 97.2% residues, respectively, were in the most favored region. The docking studies revealed that the binding energy of H. armigera was -3.50 and -7.65 kcal/mole compared to the binding energy of T. cacoeciae -2.96 and -5.28 kcal/mole for monocrotophos and fenvalerate, respectively, inferring stronger interaction of H. armigera CYP450 with the insecticides and thereby higher potential for resistance in H. armigera.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Downloads

Published

2013-03-01

How to Cite

Dhanya, K. P., Panda, M., Jalali, S. K., Krishna Kumar, N. K., Gandhi Gracy, R., Venkatesan, T., & Nagesh, M. (2013). <I>In silico</I> Docking Studies on Cytochrome P450 Enzymes of <I>Helicoverpa armigera</I> (Hubner) and <I>Trichogramma cacoeciae</I> Marchal and Implications for Insecticide Detoxification. Journal of Biological Control, 27(1), 1–9. https://doi.org/10.18311/jbc/2013/3450

Issue

Section

Research Articles

 

Most read articles by the same author(s)

1 2 3 4 5 6 7 8 > >>