Diversity of cry genes occurring in the North East

Jump To References Section

Authors

  • ICAR-National Bureau of Agricultural Insect Resources, H.A. Farm Post, Hebbal, Bellary Road, Bengaluru – 560024, Karnataka ,IN
  • ICAR-National Research Centre for Banana, Thogaimalai Rd, Podavur, Thiruchirapalli – 620102, Tamil Nadu ,IN
  • ICAR-National Research Centre for Banana, Thogaimalai Rd, Podavur, Thiruchirapalli – 620102, Tamil Nadu ,IN
  • ICAR-National Research Centre for Banana, Thogaimalai Rd, Podavur, Thiruchirapalli – 620102, Tamil Nadu ,IN
  • ICAR-National Research Centre for Banana, Thogaimalai Rd, Podavur, Thiruchirapalli – 620102, Tamil Nadu ,IN
  • ICAR-National Research Centre for Banana, Thogaimalai Rd, Podavur, Thiruchirapalli – 620102, Tamil Nadu ,IN
  • ICAR-National Research Centre for Banana, Thogaimalai Rd, Podavur, Thiruchirapalli – 620102, Tamil Nadu ,IN

DOI:

https://doi.org/10.18311/jbc/2019/23476

Keywords:

Bacillus thuringiensis, bioassay, cry genes, diversity, North East

Abstract

The search for new Bacillus thuringiensis (Bt) strains is a continuous process and researchers are now focusing on finding toxin proteins that are toxic to pests of insect orders that are not reported. In the present study soil and insect cadaver samples were collected from North East India comprising the states of Assam, Tripura and Mehhalaya and native Bt were isolated using standard protocols. At total of 30 Bt isolates were purified and characterized. Various types of crystal morphology were encountered that included bipyramidal, cuboidal, square, rhomboid, spherical and irregular. PCR analysis showed that diverse cry genes were expressed. The cry genes identified were Lepidoptera, Coleoptera and Diptera specific. Detected genes included cry1Ac, cry2A, cry4A, cry10A, cry16A, cry17A, cry19A, cry30Aa, cry44Aa, cry11A, cry4B, cry12A, cry8A and cry7A. Many of them were positive for Vip3A protein. The coleopteran specific Bt were evaluated against Sitophilus oryzae and Callosobruchus chinensis and NBAIR-AgBt6 was found to be toxic. The isolates are being further evaluated for use as biopesticides.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Downloads

Published

2019-12-05

How to Cite

Rangeshwaran, R., Velavan, V., Kumar, S., Apoorva, V., Venugopala, K. M., Shylesha, A. N., & Sivakumar, G. (2019). Diversity of <i>cry</i> genes occurring in the North East. Journal of Biological Control, 33(3), 242–252. https://doi.org/10.18311/jbc/2019/23476

Issue

Section

Research Articles
Received 2019-03-27
Accepted 2019-10-17
Published 2019-12-05

 

References

Aly AH Nariman. 2007. PCR Detection of cry genes in local Bacillus thuringiensis Isolates. Aust J Basic Appl Sci. 1(4): 461-466.

Aronson AI. 1994. Bacillus thuringiensis and its use as biological insecticide. Plant Breed Rev. 12: 19-45.

Asokan R, Mahadeva Swamy HM, Birah A, Geetha G Thimmegowda. 2013. Bacillus thuringiensis Isolates from Great Nicobar Islands. Curr Microbiol. 66: 621-626.

Baig DN, Mehnaz S. 2010. Determination and distribution of cry-type genes in halophilc Bacillus thuringiensis isolates of Arabian Sea sedimentary rocks. Microbiol Res.

(5):376-83.https:// doi.org/10.1016/j.micres.2009.08.003 PMid:19850456

Baig DN, Bukhari DA, Shakoori AR. 2010. Cry genes profiling and the toxicity of isolates of Bacillus thuringiensis from soil samples against American bollworm, Helicoverpa armigera. J Appl Microbiol.

(6): 1967-1978. https://doi.org/10.1111/j.13652672.2010.04826.x PMid:20738439

Barloy F, Lecadet M-M, Delécluse A. 1998. Distribution of clostridial cry-like genes among Bacillus thuringiensis and Clostridium strains. Curr Microbiol. 36: 232-237. https://doi.org/10.1007/s002849900300 PMid:9504991

Ben-Dov E, Zaritsky A, Dahan E, Barak Z, Sinai R, Manasherob R, A Khamraev, E Troitskaya, Dubitsky A, Berezina N, Margalith Y. 1997. Extended screening by PCR for seven cry-group genes from field-collected strains of Bacillus thuringiensis. Appl Environ Microbiol. 63: 4883-90.

Bourque SN, Valero JR, Mercier J, Lavoie MC, Levesque RC. 1993. Multiplex polymerase chain reaction for detection and differentiation of the microbial insecticide Bacillus thuringiensis. Appl Environ Microbiol. 59:(2): 523-527

Bravo A, Sarabia S, Lopez L, Ontiveros H, Abarca C, Ortiz A, Soberon M, Quintero, R. 1998. Characterization of cry genes in a Mexican Bacillus thuringiensis strain collection. Appl Environ Microbiol. 64: 4965-4972.

Chaubey MK. 2011. Combinatorial action of essential oils towards pulse beetle Callosobruchus chinensis Fabricius (Coleoptera: Bruchidae). Int J Agri Res. 6: 511-516. https://doi.org/10.3923/ijar.2011.511.516

Chen ML, Chen PH, Pang JC, Chia-Wei Lin CW, Chin-Fa HC, Hau-Yang T. 2014. The correlation of the presence and expression levels of cry genes with the insecticidal activitiesagainst Plutella xylostella for Bacillus thuringiensis strains. Toxins 6: 2453-2470. https://doi.org/10.3390/toxins6082453 PMid:25153253 PMCid:PMC4147593

Ejiofor AO, Johnson T. 2002. Physiological and molecular detection of crystalliferous Bacillus thuringiensis strains from habitats in the South Central United States. J Ind Microbiol Biotechnol. 28: 284-290 https://doi.org/10.1038/sj/jim/7000244 PMid:11986933

Federiei BA, Luthy P, Ibarra JE. 1990. Parasporal body of Bacillus thuringiensis israelensis: Structure, protein composition and toxicity. p. 349. In: de Barjac H and Sutherland DJ (Eds.). Bacterial control of mosquitoes and black flies. New Brunswick, Rutgers University Press. https://doi.org/10.1007/978-94-011-5967-8_3

Head G. 2005. Assessing the influence of Bt crops on natural enemies. Second Inter. Symp. on Biol. Control of Arthropods. Davos, Switzerland, Sept., 12-16.

Hofte H, Whiteley HR. 1989. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol. Rev. 53: 242-255.

Ito T, Ikeya T, Sahara K, Bando H. and Shin-ichiro A. 2006. Cloning and expression of two crystal protein genes, Cry30Ba1 and Cry44Aa1, obtained from a highly mosquitocidal strain, Bacillus thuringiensis subsp. entomocidus. Appl Environ Microbiol. 72:(8): 5673-5676. https://doi.org/10.1128/AEM.01894-05 PMid:16885329 PMCid:PMC1538732

Konecka E, Baranek J, Hrycak A, Kaznowski A. 2012. Insecticidal activity of Bacillus thuringiensis Strains isolated from soil and water. Scientific World J. 2012: 1-5.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent. J Biol Chem. 193: 265-75.

Md. Abdur Rashid, Bhuiyan Al Sazzad, Rowshan Ara Begum, Reza Md. Shahjahan. 2012. Mortality effect of Bt extracts and esterase variability in three stored grain insects: Callosobruchus chinensis, Sitophilus granarius and Tribolium castaneum. Int J Agric Food Sci. 2(4) : 158-163.

Morris ON, Converse V, Kanagaratnam P, Cote JC. 1998. Isolation, characterization, and culture of Bacillus thuringiensis from soil and dust from grain storage bins and their toxicity for Mamestra configurata (Lepidoptera: Noctuidae). Can Entomol. 130: 515-537. https://doi.org/10.4039/Ent130515-4

Nazarian A, Jahangiri R, Jouzani GS, Seifinejad A, Soheilivand S, Bagheri O, Keshavarzi M, Alamisaeid K. 2009. Coleopteran-specific and putative novel cry genes in Iranian native Bacillus thuringiensis collection. J Invertebr Pathol. 102: 101-109. https://doi.org/10.1016/j.jip.2009.07.009 PMid:19631215

Porcar M, Juarez-Perez VP. 2003. PCR-based identification of Bacillus thuringiensis pesticidal crystal genes.

FEMS Microbiol Rev. 26(5): 419-32. https:// doi.org/10.1111/j.1574-6976.2003.tb00624.x PMid:12586389

Quesada-Moraga E, Garcıa-Tovar E, Valverde-Garcıa P, Santiago-Alvarez C. 2004. Isolation, geographical diversity and insecticidal activity of Bacillus thuringiensis from soils in Spain. Microbiol Res. 159:(2004): 59-71. https://doi.org/10.1016/j.micres.2004.01.011 PMid:15160608

Ramalakshmi A, Udayasuriyan V. 2010. Diversity of Bacillus thuringiensis isolated from Western Ghats of Tamil Nadu State, India. Curr Microbiol. 61(1): 13-8. doi: 10.1007/ s00284-009-9569-6. https://doi.org/10.1007/s00284-0099569-6 PMid:20033169.

Rangeshwaran R, Velavan V, Frenita DL, Surabhi Kumari, Shylesha AN, Mohan M, Satendra Kumar and Sivakumar G. 2016. Cloning, expression and bioassay of Vip3A protein from an indigenous Bacillus thuringiensis isolate. J Pure Appl Microbiol. 10(2): 1533-1539

Salehi Jouzani G, Seifinejad A, Saeedizadeh A, Nazarian A, Yousefloo M, Soheilivand S, Mousivand M, Jahangiri R, Yazdani M, Amiri RM, Akbari S.2008. Molecular detection of nematicidal crystalliferous Bacillus thuringiensis strains of Iran and evaluation of their toxicity on free-living and plant-parasitic nematodes.

Can J Microbiol. 54: 812-822. https://doi.org/10.1139/W08-074 PMid:18923549

Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: a laboratory manual. 2nd edition. Cold Spring ndHarbor Laboratory, Cold Spring Harbor, N.Y.

Santana MA, Moccia-V CC, Gillis AE. 2008. Bacillus thuringiensis improved isolation methodology from soil samples. J Microbiol Methods 75:(2): 357-8. doi: 10.1016/j.mimet.2008.06.008. https://doi.org/10.1016/j.

mimet.2008.06.008 PMid:18619500

Schnepf E, Crickmore N, Van Rie J, Lereclus D, Baum J, Feitelson J, Zeigler DR, Dean DH. 1998. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol Mol Biol Rev. 62: 775-806.

Thammasittirong A, Attathom T. 2008. PCR-based method for the detection of cry genes in local isolates of Bacillus thuringiensis from Thailand. J Invertebr Pathol. 98: 121-126.

https://doi.org/10.1016/j.jip.2008.03.001 PMid:18407288 Travers RS, Martin PA, Reichelderfer CF. 1987. Selective process for efficient isolation of soil Bacillus spp. Appl Environ Microbiol. 53: 1263-1266.

Uribe D, Martinez W and Ceron J. 2003. Distribution and diversity of cry genes in native strains of Bacillus thuringiensis obtained from different ecosystems from Colombia. J Invertebr Pathol. 82: 119-127. https://doi.

org/10.1016/S0022-2011(02)00195-7

Van Frankenhuyzen K (2009) Insecticidal activity of Bacillus thuringiensis crystal proteins. J Invertebr Pathol.

: 1-16. https://doi.org/10.1016/j.jip.2009.02.009 PMid:19269294

Vidal-Quist JC, Castañera P. and González-Cabrera J. 2009. Diversity of Bacillus thuringiensis strains isolated from citrus orchards in Spain and evaluation of their insecticidal activity against Ceratitis capitata. J Microbiol Biotechnol. 19(8): 749-759.

Zothansanga, Lalhmachhuani N, Senthil Kumar N, Gurusubramanian G. 2011. PCR pathotyping of native Bacillus thuringiensis from Mizoram, India. Sci Vis. 11(3): 171-176.

Most read articles by the same author(s)

<< < 1 2 3 4 5 > >>