Eco-friendly management of lepidopteran insect pests through entomopathogenic nematodes

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Authors

  • Rashid Pervez
     Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi - 110012 ,IN
  • Uma Rao
     Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi - 110012 ,IN

DOI:

https://doi.org/10.18311/jbc/2020/23012

Keywords:

Biological control, entomopathogenic nematodes, insects, Lepidoptera

Abstract

In a recent survey, we have isolated six entomopathogenic nematodes from Hapur, Shamli, Saharanpur and Meerut districts of western Uttar Pradesh. Preliminary identification at genus level classified one isolate as Heterorhabditis sp., two isolates as Steinernema spp. and three as Oscheius spp. These six newly isolated EPNs have been designated as Oscheius sp. (IARI-EPN RP 01), Oscheius sp. (IARI-EPN RP 02), Steinernema sp. (IARI-EPN RP 03), Oscheius sp. (IARI-EPN RP 04), Steinernema sp. (IARI-EPN RP 05) and Heterorhabditis sp. (IARI-EPN RP 06). All these were evaluated for infectivity against lepidopteran larvae, Maruca vitrata infesting pigeonpea, Pieris brassicae infesting mustard and Spodoptera litura infesting chickpea. Among the tested EPNs, Steinernema sp. (IARI-EPN RP 03), Oscheius sp. (IARIEPN RP 04), Heterorhabditis sp. (IARI-EPN RP 06) were found to be promising against M. vitrata as they showed 100% mortality within 48 h followed by Oscheius sp. (IARI-EPN RP 01), Oscheius sp. (IARI-EPN RP 02) which took 72 h, while Steinernema sp. (IARI-EPN RP 05) took about 120 h to kill the insects. Oscheius sp. (IARI-EPN RP 02), Steinernema sp. (IARI-EPN RP 03) and Heterorhabditis sp. (IARI-EPN RP 06) gave 100% mortality of P. braissicae within 48 h followed by Oscheius sp. (IARI-EPN 04) and Steinernema sp. (IARI-EPN RP 05) that killed larvae after 72 h, while Oscheius sp. (IARI-EPN RP 01) took about 120 h. Lastly, Steinernema sp. (IARI-EPN RP 03) and Heterorhabditis sp. (IARI-EPN 06) were found promising for S. litura as both of them caused 100% mortality within 48 h followed by Oscheius sp. (IARI-EPN RP 01), Oscheius sp. (IARI-EPN RP 02) and Steinernema sp. (IARI-EPN RP 05) that took 72 h, while, Oscheius sp. (IARI-EPN RP 04) was effective at about 120 h. The present results indicated that both Steinernema sp. (IARI-EPN RP 03) and Heterorhabditis sp. (IARI-EPN RP 06) were highly virulent against the tested lepidopteran insect pests. Further evaluation of these new EPN isolates under field conditions will indicate their utility in integrated pest management.

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Published

2020-07-17

How to Cite

Pervez, R., & Rao, U. (2020). Eco-friendly management of lepidopteran insect pests through entomopathogenic nematodes. Journal of Biological Control, 34(1), 66–72. https://doi.org/10.18311/jbc/2020/23012

Issue

Volume 34, Issue 1, March 2020

Section

Research Articles
Crossref
Scopus
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Europe PMC
Received 2018-12-18
Accepted 2019-01-01
Published 2020-07-17

 

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