Isozymes and Protein Profiles of Four Species of Trichogramma Westwood (Trichogrammatidae:Hymenoptera)

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Authors

  • Division of Entomology, Division of Nematology, Indian Agricultural Research Institute, New Delhi - 110 012 ,IN
  • Division of Entomology, Division of Nematology, Indian Agricultural Research Institute, New Delhi - 110 012 ,IN
  • Division of Entomology, Division of Nematology, Indian Agricultural Research Institute, New Delhi - 110 012 ,IN
  • Division of Entomology, Division of Nematology, Indian Agricultural Research Institute, New Delhi - 110 012 ,IN

DOI:

https://doi.org/10.18311/jbc/1991/15210

Keywords:

Trichogramrna spp., Isozymes, Protein Profiles.

Abstract

Electrophoresis of proteins and isoenzymes, b esterase and Malate dehydrogenase (MDH) was carried out at the Division of Entomology, Indian Agricultural Research Institute, New Delhi with four species of Trichogramma viz., T. japonicum Ashmead, T. chilonis Ishii, T. brasiliensis Ashmead and T. minutum Riley with a view to differentiate these species based upon their electrophoretic patterns. Species specific qualitative and quantitative differences were observed in the protein fractions of the four species. The species specific protein fraction observed were 5 in T. japonicum, 2 in T. chilonis and one in T. brasiliensis. There were more protein bands common to T. chilonis and T. minutum. In all the species, three different b esterases with recognizable Rm values were observed but for MDH only one band was observed in all the species, and their Rm values also did not differ significantly. For differentiating these four species, electrophoresis of proteins and b esterase could be employed as a dependable tool.