Characterization of Lysinibacillus Sphaericus C3-41 Strain Isolated from Northern Karnataka, India that is Toxic to Mosquito Larvae

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Authors

  • College of Agriculture, Bheemarayanagudi, Yadgir, Karnataka ,IN
  • College of Agriculture Raichur, Karnataka ,IN
  • Microbial Sciences Division, Agharkar Research Institute, Pune, Maharashtra ,IN
  • Indian Institute of Technology, Madras, Tamil Nadu ,IN
  • College of Agriculture, Bheemarayanagudi, Yadgir, Karnataka ,IN

DOI:

https://doi.org/10.18311/jbc/2014/14934

Keywords:

Lysinibacillus (Bacillus) Sphaericus, Biochemical Tests, 16s rRNA Gene Sequence, Culex Quin Quefasciatus, Bioassay

Abstract

One of the eco-friendly approaches to control mosquitoes is by the use of <i>Lysinibacillus sphaericus</i>. <i>L. sphaericus</i> (previous name <i>Bacillus sphaericus</i>) C3-41 (CP000817) was isolated from Rabbanahalli village of Yadgir district, Karnataka, India and was identified by biochemical and molecular means. Biochemical tests established its identity as <i>L. sphaericus</i>. It is also clear from the phylogenetic analysis that 16S ribosomal RNA nucleotide sequence of <i>L. sphaericus</i> C3-41 (CP000817) showed 99.73 per cent similarity with reference strain. Bioassay of <i>L. sphaericus</i> against second instar larvae of Culex quinquefasciatus recorded significantly higher mortality of 100 per cent at 0.075 % CSM. When assayed against second instar larvae of Anopheles subpictus mortality was 83.33 per cent at 0.075 % CSM.This new strain of L. sphaericus isolated from Northern dry zone of Karnataka, where the temperature ranges from 38°C to 46°C, could be a better biological pesticide to tackle the Culex mosquito and prevent diseases transmitted by it, particularly in Northern Karnataka.