Volume 3, Issue 1, 2017   |   https://doi.org/10.18311/jade/2017/16448   |  Cited by 0 articles

Hard Tissue Architecture of Tooth – No Strain with Stain


  • Penang International Dental College, Department of Oral Biology, Salem, Tamil Nadu, 636308, India
  • Penang International Dental College, Department of Oral Biolog, Salem, Tamil Nadu, 636308, India
  • Vinayaka Mission's Sankarachariyar Dental College, Department of Oral Pathology, Salem, Tamil Nadu, 636308, India


One of the methods for visualizing and studying the hard tissues of the tooth is by preparation of ground sections of the desired area of the tooth. This method is relatively simple and enables us to appreciate the site and arrangement of various anatomical units of enamel, dentin and cementum. However, these sections are unstained and the features observed are based on the degree of light permeating through the sections. In this experiment, natural tooth were preserved in haematoxylin and eosin to study histological components of tooth. Twenty recently extracted natural teeth were utilized for this experiment and divided into two groups. In group 1, apical portion of the tooth were sealed and perforations were made on the different aspects of the tooth. In group 2, the apical portions of the root were flattened and the apex kept open. Both groups were preserved in haematoxylin and eosin to permit imbibition of the stain. Following which, ground sections were prepared and viewed under a microscope.


Haematoxylin and Eosin Stains, Histological Components, Ground Section.

Subject Discipline


Full Text:


Nanci A. Ten Cate’s oral histology. 8th ed. Elsevier Publications. 2013; p. 155–9. PMid:24421827 PMCid: PMC3852453

Avery JK. Oral development and histology. 3rd ed. Thieme Publications. 2002; p. 163–5.

Kumar GS. Orban’s Textbook of Oral Histology. 12th ed. Elsevier publications; Mosby. 2010. p. 95.

Singh A, Gorea RK, Urveshisingla. Few tips for making ground sections of tooth for research purpose: A review. Journal of Punjab Academy of Forensic Medicine and Toxicology. 2006; 6:11–3.

Williamson RA. Histological preparation of teeth and tooth growth. A review. Archives of Oral and Dental Research. 2015; 2:1–5.


  • There are currently no refbacks.