Total Phenolic Content of Organic and Conventionally Grown Gourd Vegetables


Affiliations

  • PSG College of Arts and Science, Coimbatore, 641 014, India

Abstract

The increasing consumers’ demands to acquire healthier fruits and vegetables as well as the urgency in looking to natural compounds with Antioxidant Activity (AOA) has encouraged a quick expansion of research studies about phenols in vegetables. Gourd vegetables refer to the fruits of plants in the two Cucurbitaceae genera Lagenaria and Curcubita. There is a diverse source of polyphenols in plant materials. Seven gourd vegetables grown conventionally (CV) were collected from the local market and a set of the same vegetables from certified organic farms (OG). They were analysed for the Total Phenol (TP) content by the Folin-Ciocalteu method using Gallic acid as standard. Earlier each vegetable was extracted with ethanol, methanol and water separately for the estimation of TP. The TP content was expressed as μg of GAE/g of FW. Bitter gourd had the highest amounts of TP in both the conventional (1766.52 μg of GAE/g of FW)) and organic (1962.0 μg of GAE/g of FW)) samples. Conventional gourd vegetables had more TP when compared to organic varieties. Among the solvents, the extraction of phenols was the highest in water followed by methanol and ethanol. Gourd vegetables are widely available and can contribute significant amounts of phenols to the diet.

Keywords

Total phenolics; Folin-Ciocalteu method; gourd vegetables; organic farming; ethanol, methanol, water solvents

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References

Crozier, A., Jaganath, I.B. and Clifford, M.N. Phenols, polyphenols and tannins: An overview. In Plant Secondary Metabolites: Occurrence, Structure and Role in the Human Diet; Crozier, A., Clifford, M., Ashihara, H., Eds.; Blackwell: Oxford, UK, 2006, pp. 1-24.

D”Archivio, M., Filesi, C., Vari, R., Scazzocchio, B. and Masella, R. Bioavailability of the polyphenols: status and controversies. Int. J. Mol. Sci., 2010, 11, 1321-1342.

Tsao, R. Chemistry and biochemistry of dietary polyphenols. Nutr., 2010, 2, 1231-1246. doi:10.3390/nu2121231

Jallali, I., Megdiche, W., M’Hamdi, B., Oueslati, S., Smaoui, A., Abdelly, C. and Ksouri, R. Changes in phenolic composition and antioxidant activities of the edible halophyte Crithmum maritimum L. with physiological stage and extraction method. Acta. Physiologiae. Plantarum. 2012, 34,1451-1459. doi:10.1007/s11738-012-0943-9.

Zhao, F., Watanabe, Y., Nozawa, H., Daikonnya, A., Kondo, K. and Kitanaka, S. Prenyl flavonoids and phloroglucinol derivatives from hops (Humulus lupulus). J. Natural Prod., 2005, 68, 43-49. doi:10.1021/np0499113.

Sun, B., Leandro, M.C., de Freitas, V. and Spranger, M.I. Fractionation of red wine polyphenols by solid-phase extraction and liquid chromatography. J. Chromatography., 2006, 1128, 27-38. doi:10.1016/j.chroma.2006.06.026.

Greenfield, H. and Southgate, D.A.T. Food composition data: production, management and use. London. Elsevier Applied Science, 2003.

Garcia-Salas, P., Morales-Soto, A., Segura-Carretero, A. and Fernández-Gutiérrez, A. Phenolic compound-extraction systems for fruit and vegetable samples. Molecu., 2010, 15, 8813. doi:10.3390/molecules15128813

Wong, P.Y.Y. and Kitts, D.D. Studies on the dual antioxidant and antibacterial properties of parsley (Petroselinum crispum) and cilantro (Coriandrum sativum) extracts. Fd. Chem., 2006, 97, 505-515. [CrossRef]

Rasheed, A.A., Cobham, E.I., Zeighami, M. And Ong, S.P. Extraction Of phenolic compounds from pineapple fruit, The 2nd International Symposium on Processing and Drying of Foods, Vegetables and Fruits (ISPDFVF 2012) University of Nottingham, Malaysia Campus, 18th – 19th June 2012.

Ainsworth, E.A. and Gillespie, K.M. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin- Ciocalteu reagent. Nat. Protoc., 2007, 2, 875-877.

Armstrong, W.P. The wild and wonderful world of gourds. Zoonooz., 1996, 10, 24-27.

Koffi, E., Sea, T., Dodehe, Y. and Soro, S. Effect of solvent type on extraction of polyphenols from twenty three ivorian plants. J. Anim. Plant Sci., 2010, 5, 550-558.

Budrat, P. and Shotipruk, A. Extraction of phenolic compounds from fruits of bitter melon (Momordica charantia) with subcritical water extraction and antioxidant activities of these extracts, Chiang Mai. J. Sci., 2008, 35, 123-130.

Myojin, C., Enami, N., Nagata, A., Yamaguchi, T., Takamura, H. and Matoba, T. Changes in the radical scavenging activity of bitter gourd (Momordica charantia L.) during freezing and frozen storage with or without blancing. J. Fd. Sci., 2008, 73, 546-550.

Aminah, A. and Anna, P.K. Influence of ripening stages on physicochemical characteristics and antioxidant properties of bitter gourd (Momordica charantia). Int. Fd. Res. J., 2011, 18, 895-900.

Kubola J. and Siriamornpun, S. Phenolic contents and antioxidant activities of bitter gourd (Momordica charantia L.) leaf, stem and fruit fraction extracts in vitro. Fd. Chem., 2008, 110, 881-890. doi: 10.1016/j.foodchem.2008.02.076.

Wu, S.J. and Ng, L.T. Antioxidant and free radical scavenging activities of wild bitter melon (Momordica charantia Linn. Var. abbreviates Ser.) in Taiwan. LWT - Fd. Sci. Technol., 2008, 41, 323-330.

Horax, R., Hettiarachy, N. and Islam, S.Total phenolic contents and phenolic acid constituents in four varieties of bitter melons (Momordica charantia) and antioxidant activities of their extracts. J. Fd. Sci., 2005, 70, 275-280.

Rezaeizadeh, A., Zuki, A.B.Z., Abdollahim N., Goh, Y.M., Noordin, M.M., Hamid, M. and Azmi, T.I. Determination of antioxidant activity on methanolic and chloroformic extracts of Momordica charantia. Af. J. Biotechnol., 2011, 10, 4932-4940.

Isabella, M., Lee, B.L., Lim, M.T., Koh, W.P., Huang, D. and Ong, C.N. Antioxidant activity and profiles of common vegetables in Singapore, Fd. Chem., 2010, 120, 993-1003.

Sirohi, P.S., Choudary, B. and Kalda, T.S. Pumpkin “Pusavishwas” for tropical and subtropical region. Indian Horticulture, 1991, 36, 24-26.


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