Assessment of Aflatoxin Contamination from Discoloured Kernels in Ready-To-Eat Processed Groundnut Products


Affiliations

  • National Institute of Nutrition (ICMR), Food Toxicology Division, Hyderabad, Telangana, 500 007, India

Abstract

The present study was attempted to assess the extent of aflatoxin contamination from presence of discoloured kernels (DKs) in ready-to-eat (RTE) processed whole groundnut products sold in Hyderabad city in southern India. A total of 34/56 fried (FG) and roasted and salted groundnut (RSG) samples were examined for the presence of DKs. DKs were segregated from the samples and subjected to aflatoxin analysis either as single DK or pooled DKs using HPLC methods. The aflatoxin content in the RTE samples was computed from the aflatoxin levels determined in the segregated DKs as well as in the non-discoloured kernels (NDKs) and ingredients used for coating such as flour and spices. Analysis of 77 single DKs indicated presence of aflatoxins in 31% of the DKs at levels ranging from 0.007 to 1383.4 μg/g. Analysis of 17 pooled DK samples indicated presence of aflatoxins in 13 pooled DKs at levels ranging from 0.142-357.3 μg/ pooled DKs. The total aflatoxin content in the RTE samples calculated from aggregate of aflatoxin levels in DKs, NDKs and other components ranged from 0.001 to 2.779 μg/g sample respectively and was contributed mostly by DKs (90%). Aflatoxin was not detected in 22 samples that did not contain DKs. Presence of DKs in RTE groundnut products for direct human consumption can become potential sources of aflatoxin exposure to the consumer and methods to prevent their entry into processed foods containing whole kernels at post processing is urgently required.

Keywords

Aflatoxin contamination, ready-to-eat groundnut, discoloured kernels, segregation, fried, roasted and salted products

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References

Wild, C.P., Miller, J.D. and Groopman, J.D. Mycotoxin control in low- and middle-income countries. International Agency for Research on Cancer (IARC), 2015, Lyon, France.

Krishnamachari, K.A., Nagarajan, V., Bhat, R.V. and Tilak, T.B. Hepatitis due to aflatoxicosis- an outbreak in Western India. Lancet, 1975, 305, 1061-1063.

Wild, C.P. and Gong, Y.Y. Mycotoxins and human disease: a largely ignored global health issue. Carcinogenesis, 2010, 31, 71-82.

Liu, Y. and Wu, F. Global burden of aflatoxin-induced hepatocellular carcinoma: a risk assessment. Environ. Health. Perspect., 2010, 118, 818-824.

Watson, S., Gong, Y.Y. and Routledge, M. Interventions targeting child under nutrition in developing countries may be undermined by dietary exposure to aflatoxin. Crit. Revs. Fd. Sci. Nutr., 2015, 57, 1963-1975.

Mupunga, I., Mngqawa, P. and Katerere, D.R. Peanuts, aflatoxins and under nutrition in children in sub-saharan Africa. Nutri., 2017, 9, 1287.

World Health Organization (WHO). WHO estimates of the global burden of food borne diseases: food borne disease burden epidemiology reference group. 2007-2015. WHO, 2015. http://www.who.int/foodsafety/publications/foodborne_disease/fergreport/en/ (accessed on 17 January 2018).

Food and Agriculture Organization (FAO). Worldwide regulations for mycotoxins in food and feed in 2003. FAO Food and Nutrition Paper No. 81. Rome. 2004.

Vasanthi, S. and Bhat, R.V. Mycotoxins in food-Occurrence, health and economic significance and food control measures. Ind. J. Med. Res., 1998, 108, 222-224.

Codex Alimentarius Commission. Standard for peanuts. CODEX STAN 200-1995.

Codex Committee on Contaminants in Foods (CCCFS). Proposed draft maximum level for total aflatoxins in ready-to-eat peanuts and associate sampling plan. CX/CF 15/9/9. 9th Session New Delhi, India, 16 – 20 March 2015.

Food Safety and Standards Authority of India (FSSAI). Compendium of Food Safety and Standards (Contaminants, Toxins and Residues) Regulation. 2011. http://www.fssai.gov.in/ home/fss-legislation/fss-regulations.html (accessed 2 January 2018).

Settaluri, V.S., Kandala, C.V.K., Puppala, N. and Sundaram, J. Peanuts and their nutritional aspects- A review. Fd. Nutr. Sci., 2012, 3, 1644-1650.

Waliyar, F., Osiru, M., Ntare, B.R. et al. Post-harvest management of aflatoxin contamination in groundnut. World Mycotoxin J., 2015, 8, 245-252.

Codex Alimentarius Commission. Code of Practice for the prevention and reduction of aflatoxin contamination in peanuts. CAC/RCP 55-2004.

Dorner, J.W. Management and prevention of mycotoxins in peanuts. Fd. Addit. Contam., 2008, 25, 203-208.

Galvez, F.C.F., Francisco, L.D.L., Villarino, J. et al. Manual sorting to eliminate aflatoxin from peanuts. J. Fd. Prot., 2003, 66, 1879-1884.

Whitaker, T.B. Efficiency of the blanching and electronic color sorting process for reducing aflatoxin in raw shelled peanuts. Peanut Sci., 1997, 24, 62-66.

Chiou, R.Y.Y. and Tsao, H.H. Aflatoxin content of single peanut kernels in commercial lots and in kernels artificially infected with Aspergillus parasiticus. J. Fd. Prot., 1997, 60, 843-848.

Florkowski, W.J. and Kolavalli, S. Aflatoxin control strategies in the groundnut value chain in Ghana. Ghana Strategy Support Program (GSSP) Working Paper 33. Washington DC. International Food Policy Research Institute (IFPRI). 2013. http://ebrary.ifpri.org/cdm/ref/collection/p15738coll2/id/127749 (accessed 12 September 2016).

Xu, Y., Doel, A., Watson, S. et al. Study of an educational hand sorting intervention for reducing aflatoxin B1 in groundnuts in rural Gambia. J. Fd. Prot., 2017, 80, 44-49.

Ambarwati, S., Dharmaputra, O.S. and Retnowati, I. Dietary exposure assessment for aflatoxin B1 from processed peanut products in municipality of Bogor. BIOTROPIA., 2011, 18, 1- 12.

Karlovsky, P., Suman, M., Berthiller, F., De Meester, J. et al. Impact of food processing and detoxification treatments on mycotoxin contamination. Mycotoxin Res., 2016, 32, 179-205.

Leong, Y-H., Ismail, N., Latif, A.A. and Ahmad, R. Aflatoxin occurrence in nuts and commercial nutty products in Malaysia. Fd. Control., 2010, 21, 334-338.

Oliveira, C.A.F., Gonçalves, N.B., Rosim, R.E. and Fernandes, A.M. Determination of aflatoxins in peanut products in the Northeast Region of São Paulo, Brazil. Int. J. Mol. Sci., 2009, 10, 174-183.

Arya, S.S., Salve, A.R. and Chauhan, S. Peanuts as functional food: a review. J. Fd. Sci. Technol., 2016, 53, 31-41.

Nautiyal, P.C. Groundnut: Post-harvest operations. Information on post-harvest operations (INPhO) Post-harvest Compendium. Food and Agriculture Organization, 2002, 126pp. http://www.fao.org/fileadmin/user_upload/inpho/docs/Post_Harvest_Compendium__Groundnut.pdf (accessed on 14th September 2016).

Kumar, V., Bagwan, N.B., Koradia, V.G. and Padavi, R.D. Colour sorting - an effective tool to remove aflatoxin contaminated kernels in groundnut. Ind. Phytopathol., 2010, 63, 449451.

Trucksess, M.W. Aflatoxins in peanuts and peanut products. AOAC Official Method 970.45. In: Horwitz, W. and Latimer, G.W. (eds). Official Methods of Analysis of AOAC International 18th Edition. 2005.

Trucksess, M.W. Aflatoxin in corn and peanut butter liquid chromatography method. AOAC Official Method 990.33 In: Horwitz, W. and Latimer G.W. (eds). Official Methods of Analysis of AOAC International 18th Edition. 2005.

Dorner, J.W. and Cole, R.J. Distribution of aflatoxin in grade sample components of farmer’s stock peanuts. Peanut Sci., 1997, 24, 47-51.

Begum, M.A.J., Venudevan, B. and Jayanthi, M. Storage fungi in groundnut and the associate seed quality deterioration- A review. Plant. Pathol. J., 2013, 12, 127-134.

Cucullu, A.F., Lee, L.S., Mayne, R.Y. and Goldblatt, L.A. Determination of aflatoxin in individual peanuts and peanut sections. J. Amer. Oil. Chem. Soc., 1966, 43, 89-92.

Pelletier, M.J. and Reizner, J.R. Comparison of fluorescence sorting and color sorting for the removal of aflatoxin from large groups of peanuts. Peanut Sci., 1992, 19, 15-20.

Lee, L.S., Yatsu, L.Y. and Goldblatt, L.A. Aflatoxin contamination: electron microscopic evidence of mold penetration. J. Amer. Oil. Chern. Soc., 1967, 44, 331-332.


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