Estimation of Gatifloxacin in Pharmaceutical Dosage Forms by High Performance Liquid Chromatography


  • V. V. Institute of Pharmaceutical Science, Gudlavalleru, A. P., 521356, India
  • College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, A. P., 530003, India


A simple, rapid, sensitive and precise High Performance Liquid Chromatographic (HPLC) method has been developed for the estimation of gatifloxacin in its pharmaceutical dosage forms. In this method RP - C 18 column (150mmx4.6mm I.D., 5 μm particle size) with mobile phase consisting of acetonitrile and 0.05M phosphate buffer in the ratio of 25:75 v/v in isocratic mode was used. The detection wavelength is 293nm and the flow rate is 0.8ml/min. Ciprofloxacin is used as internal standard. The linearity was fo und to be in the range of 0.1 to 10 μg/ml and shows a correlation coefficient of 0.9999. The intra - and inter - day variation was found to be less than 1.2% showing high precision of the assay method. The mean percent recovery of the drug from the solution c ontaining 4 μg/ml was 100.5 ± 2.06 indicating high accuracy of the proposed method. Hence, this method is simple, fast, specific, accurate, precise and less time consuming for the estimation of gatifloxacin in pharmaceutical dosage forms.


HPLC, Gatifloxacin, Estimation, Pharmaceutical Dosage Forms.

Subject Discipline

Pharmacy and Pharmacology

Full Text:


The Merck Index, 13th edition, Merck & Co., Whitehouse Station, NJ, 2001; 4388: 777.

Perry, Caroline M, Barman B, Julia A and Harriet M, Gatifloxacin, Drugs, 1999; 58: 683-696.

Goodman and Gilmans, The Pharmacological Basis of Therapeutics, 11th edition, Mcgraw-Hill MedicalPublishing Division, New York, 2006, 1119.

Changchuan G, Peng D, Zhijie C, Lie W and Wei J, Rapid determination of gatifloxacin in biological samples and pharmaceutical products using europiumsensitized fluorescence spectrophotometry, Luminescence, 2008; 23: 7-13.

Xiashi Z, Aiqin G and Suhai Y, Fluorescence probe enhanced spectrofluorimetric method for the determination of gatifloxacin in pharmaceutical formulations and biological fluids, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2008; 69: 478-482.

Suhagia B N, Shah S A, Rathod I S, Patel H M, Shah D R and Marolia B P, Determination of gatifloxacin and ornidazole in tablet dosage forms by HPTLC, Anal. Sci., 2006; 22: 743-747.

Leandro T and Teresa D C, High performance liquid chromatography for quantification of gatifloxacin in rat plasma following automated on-line solid phase extraction, J. Pharm. Biomed. Anal., 2007; 44: 205-210.

Sonali P, Lata K, Asha T and Deshpande A D, Simultaneous RP-HPLC estimation of gatifloxacin and ornidazole in tablet dosage forms, Ind. J. Pharm. Sci., 2007; 69: 525-528.

Al-Dgither S, Naseeruddin Alvi S and Hammami M H, Development and validation of an HPLC method for the determination of gatifloxacin stability in human plasma, J. Pharm. Biomed. Anal., 2006; 41: 251-255.

Salgado H R N Lopes C C G O, Determination of gatifloxacin in bulk and tablet preparations by HPLC, J. AOAC Int., 2006; 89: 642-645.

Santoro M I R M, Kassab N M, Singh A K and Kedor-Hackmam E R M, Quantitave determination of gatifloxacin, levofloxacin, lomefloxacin and pefloxacin fluoroquinolonic antibiotics in pharmaceutical preparations by HPLC, J. Pharm. Biomed. Anal., 2006; 40: 179-184.

Carazos M L S, Gonzalez L Y C, Lerma G G and Torres N W, Determination of gatifloxacin in semen by HPLC with diode-array and fluorescence detection, Chromatographia, 2006; 63: 605-608.

Overholser B R, Kays M B and Sowinski K M, Determination of gatifloxacin in human serum and urine by HPLC with ultraviolet detection, J. Chromatogr. B, 2003; 798: 167-173.

Borner K, Hartwig H and Lode H, Determination of gatifloxacin in human serum and urine by HPLC,

Chromatographia, 2000; 52: S105-S107.

Hisao K, Hajimu K and Ryozo I, HPLC determination of gatifloxacin, a new quinolone, in biological fluids, Japanese J. Chemotherapy, 1999; 47(2): 104-111.


  • There are currently no refbacks.