Anxiolytic and Anticonvulsant Activity of Alcoholic Extract of Heart Wood of Cedrus Deodara Roxb in Rodents
The present study was undertaken to evaluate the anxiolytic and a nti-convulsant activity of the alcoholic extract of heart wood of Cedrus deodara (ALCD). 50,100 and 200 mg/kg of alcoholic extract of Cedrus deodara (ALCD) were tested for its anxiolytic and anticonvulsant activity. Elevated plus maze model (EPM), Actophotometre, Light- dark model were used for testing anxiolytic activity and Pentylene tetrazole (PTZ) induc ed convulsions and Maximal electro shock (MES) induced convulsions models in mice were used for the assessment of its anticonvulsant activity, Pretreatment with ALCD and estimation of GABA in rat brain tissues also performed to study the effect of ALCD (30, 100 mg/kg) on GABA levels of brain. In Actophotometre test, higher doses (100 and 200mg/kg) of ALCD has show ed significant CNS depression by reducing locomotor activity in mice. In EPM the 100 and 200mg/kg of ALCD has increased the time spent in the open arm and decreased tim e spent in the closed arm. In Light-dark model the 100 and 200mg/kg of ALCD has showed significant inc rease in the time spent in light zone when compare to the dark zone. In PTZ induced convulsions model 100 and 200 mg/kg of ALCD has increased the onset of clonus and tonic seizures.
In MES induced convulsions model 100 and 200 mg/kg of ALCD has decreased duration of tonic extensor phase and also at 100 and 200 mg/kg doses the AL CD has increased the percentage protection in PTZ and MES induced convulsions. The ALC D (30 and 100 mg/kg) also showed significant modulation of GABA levels of cereb ellum and whole brain other than cerebellum. In conclusion these observations suggest tha t 100 and 200 mg/kg doses of ALCD are having good anxiolytic and anticonvulsant activity.
Thakur V.D, Mengi S.A. Neuropharmacological profile of Eclipta alba (Linn.) Hassk. J. Ethnopharmacol 2005.
Emamghoreishi M, Khasaki M, Aazam M.F. Coriander sativum: evaluation of its anxiolytic effect in the elevated plus-maze. J. Ethnopharmacol 2005; 96:365-70.
Rabbani M, Sajjadi S.E, Vaseghi G, Jafarian A. Anxiolytic effect of Echium amoenum on the elevated plus-maze model of anxiety in mice. Fitoterapia 2004; 75:457-64.
Nadkarni KM, Nadkarni AK, Editors. Indian Materia Medica; Bombay; Popular prakashan: 1996.
Kirtikar KR., Basu BD. Indian medical plants Blaster E., Caius JF., Bhaskar KS. (Eds). Periodical Experts Book Agency, New Delhi.1991.
Agarwal PK, Rastogi. Terpenoids from Cedrus deodara. J Ethnopharmacol. 1981; 20(6); 1391-21.
Khandelwal KR. Practical Pharmacognosy techniques and experiments.Pune, India; Nirali Prakashan; 1996.
OECD 2001-gudeline on acute oral toxicity (AOT) Environmental health and safety monograph series on testing and adjustment No.425.
Hogg SA. Review of the validity and Variability of the elevated plus-maze as an animal model of anxiety. Pharmacol Biochem Behav 1996; 54:21-30.
Rodgers RJ. Johnson NJT. Behaviorally selective effects of neuroactive steroids on plusmaze anxiety in mice. Pharmacol Biochem Behav1998; 59:221-32.
Pellow S., File SE. Anxiolytic and axiogenic drug effects on exploratory activity in an elevated plus-maze: a novel test of anxiety in the rat. Pharmacol Biochem Behav 1986; 24:525-529.
Crawley J., Goodwin FK. Pharmacol Biochem Behav 1980; 13:167.
Alagarswamy V, Thangathiruppathy A, Mandal SC, Rajasekaran S, Vijaykumar S, Revathi, et al. Pharmacological evaluation of 2-substituted (1,3,4) thiadiazolo quinazolines. Ind. J. Pharm Sci 2006; 68(1):108-11.
Khosla P., Pandhi P. Anticonvulsant effect of nimodipine alone and in combination with diazepam on PTZ induced convulsions. Ind. J. Pharmacol 2001; 33:208-211.
Vogel H.G, Vogel WH. (Eds) Drug discovery and Evaluation Pharmacological assays (Springer). 2000; 2: (E) 230-244.
Suher M.A., Abdullah S.E., Saleem S.A., Samir F.S., Amina S.A. Effects of acute and chronic Triazolam treatments on brain GABA levels in albino rats. Acta neurobiologiae experimentalis 2000; 60: 447-455.
Belzung C, Griebel G. Measuring normal and pathological anxiety-like behaviour in mice: a review. Behav. Brain Res. 2001; 125(1-2):141-149.
Noel NW, Joseph AA, Helen OK, Steven SG, Asa A. Anti-seizure activity of the aqueous leaf extract of Solanum nigrum linn (solanaceae) in experimental animals. African Health Sciences 2008; 8(2): 74-79.
Marjan NA., Schwann SR., Farzaneh Z. Anticonvulsant effects of aerial parts of Passiflora incarnata extract in mice: involvement of benzodiazepine and opioid receptors. Biomed Central 2007;7:26. 1-6.
Madhavan V, Hemalatha HT, Anita M, Yoganarasimhan S.N. Antiepileptic activity of alcohol and aqueous extracts of roots and rhizomes of smilax zeylanica linn. Pharmacologyonline 2008: 3: 263-272.
Atif A, Farhan JA, Krishna KP, Divya V. Amiloride protects against pentylenetetrazoleinduced kindling in mice. Br. J. Pharmacol 2005; 145; 880–884.
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