Pharmacognostic Standardization, Phenolic and in Vitro Antioxidant Activity of Silene villosa (Family: Caryophyllaceae)


  • College of Pharmacy, Prince Sattam bin Abdulaziz University, Department of Pharmacognosy, Al-Kharj, 11942, Saudi Arabia


The present study was designed to explore some of the quality principles and in vitro antioxidant exploration of Silene villosa “a non-intentional” herb. The pharmacognostical study and phytochemical evaluation of the herbs of S. villosa was carried out following the reported methods. The total phenolic contents and antioxidant potential of the methanol extract was also evaluated applying the standard methods. Microscopic features of the Transverse Section (T.S) of the root were observed as epidermis, medullary rays, xylem cells, phloem cells. The microscopic study of the powder showed the presence of spiral vessels, lignified fibre or tannin containing cells. The extractive value showed the water soluble materials were higher than the alcohol soluble materials. Primarily phytochemical analysis and total phenolic contents (11.78±0.169 %) in powder and extract respectively were conducted. The methanol extract of S. villosa showed dose dependent antioxidant activity. The present study helps in authentication of S. villosa and enhances further exploration of the health benefits due to the presence of phenolic contents and antioxidant property.


Antioxidants, Pharmacognostical, Phytochemicals, Silene villosa, Total Phenols

Full Text:


Alyemeni NM. Ecological studies on sand dunes vegetation in AI-Kharj region. Saudi J Biol Sci. 2000; 7(1):64–87.

Ghonime M, Emara M, Shawky R, Soliman H, El-Domany R, Abdelaziz A. Immunomodulation of RAW 264.7 murine macrophage functions and antioxidant activities of 11 plant extracts. Immunol Invest. 2015; 44 (3):237–52. PMid:25564700.

Krishnaiah D, Sarbatly R, Bono A. Phytochemical antioxidants for health and medicine: A move towards nature. Biotechnol Mol Biol Rev. 2007; 1:97–104.

Ahmad M, Kaloo ZA, Ganaie BA, Ganaie HA, Singh S. Phytochemical screening of meconopsis aculeata royle an important medicinal plant of Kashmir Himalaya: A perspective. Res J Phytochem. 2016; 10:1–9.

Heinrich M. Ethnobotany and its role in drug development. Phytother Res. 2000; 14(7):479–88.<479::AID-PTR958>3.0.CO;2-2

Hertog MGI, Hollman PH, Katan MB, Kromhout D. Intake of potentially anticarcinogenic flavonoids and their determinants in adults in the Netherlands. Nutr Cancer. 1993; 20:21–9. PMid:8415127.

Osawa T, Kavakishi S, Namiki M, Kuroda Y, Shankal DM, Waters MD. Antimutagenesis and Anticarcinogenesis Mechanisms II. Plenum; New York. 1990. p. 139–53. PMid:2183766.

Ankad GM, Konakeri RT, Hegde HV, Roy S. Variation in pharmacognostic characters and polyphenolic contents among four species of medicinal plants from the genus spermacoce (Rubiaceae). Indian J Pharm Sci. 2015; 77(4):446–52. PMid:26664061 PMCid:PMC4649792.

Foudah AI, Alam A, Soliman GA, Salkini MA, Yusufoglu, HS, Pharmacognostical, antibacterial and antioxidant studies of aerial parts of pulicaria somalensis (Family: Asteraceae). Asian J Biol Sci. 2016; 9:19–26.

Harborne JB. A guide to modern technique of plant analysis. Phytochemical Methods. London: Chapman and Hill; 1992. p. 279.

Sofowara EA, Medicinal plants and traditional medicine in Africa. Ibadan, Nigeria: Spectrum Books Ltd; 1993. p. 191–89.

Evans WC. Trease and evans pharmacognosy. 14th ed. London: WB Saunders Ltd; 1996. p. 119–59. PMid:8929253

Madaan R, Bansal G, Kumar S, Sharma A. Estimation of total phenols and flavonoids in extracts of actaea spicata roots and antioxidant activity studies. Indian J Pharm Sci. 2011; 73(6):666–9. PMid:23112402 PMCid:PMC3480753.

Barros L, Falcao S, Baptista P, Freire C, Vilas-Boas M, Ferreira IC. Antioxidant activity of Agaricus sp. mushrooms by chemical, biochemical and electrochemical assays. Food Chem. 2008; 111:61–6.

Nariya PB, Bhalodia NR, Shukla VJ, Acharya R, Nariya MB. In vitro evaluation of antioxidant activity of Cordia dichotoma (Forst f.) bark. Ayu. 2013; 34(1):124–8. PMid:24049418 PMCid:PMC3764870.

Fazal H, Ahmad N, Abbasi BH. Identification, characterization, and palynology of high-valued medicinal plants. Scientific World J. 2013:1–9. PMid:23844389 PMCid:PMC3691918

Akbar S, Hanif U, Ali J, Ishtiaq S. Pharmacognostic studies of stem, roots and leaves of Malva parviflora L. Asian Pac J Trop Biomed. 2014; 4(5):410–5. PMid:25182728 PMCid:PMC3985058.

Alam F, Saqib QNU. Pharmacognostic study and development of quality control parameters for fruit, bark and leaf of Zanthoxylum armatum (Rutaceae). Anc Sci Life. 2015; 34(3):147–55. PMid:26120229 PMCid:PMC4458905.

Ajazuddin, Saraf S. Evaluation of physicochemical and phytochemical properties of Safoof-E-Sana, a Unani polyherbal formulation. Pharmacognosy Res. 2010; 2(5):318–22. PMid:21589760 PMCid:PMC3093045.

Chumbhale D, Upasani C. Pharmacognostic standardization of stems of Thespesia lampas (Cav.) Dalz & Gibs. Asian Pac J Trop Biomed. 2012; 2(5):357–63.

Fattahi S, Zabihi E, Abedian Z, Pourbagher R, Motevalizadeh, Ardekani A, Mostafazadeh A, Akhavan-Niaki H. Total phenolic and flavonoid contents of aqueous extract of stinging nettle and in vitro antiproliferative effect on Hela and BT-474 cell lines. Int J Mol Cell Med. 2014; 3(2):102–7. PMid:25035860 PMCid:PMC4082812.


  • There are currently no refbacks.